Flavinated SDHA underlies the change in intrinsic optical properties of oral cancers

Tomoko Marumo; Chima V. Maduka; Evran Ural; Ehsanul Hoque Apu; Seock-Jin Chung; Koji Tanabe; Nynke S. van den Berg; Quan Zhou; Brock A. Martin; Tadashi Miura; Eben L. Rosenthal; Takahiko Shibahara; Christopher H. Contag

Flavinated SDHA underlies the change in intrinsic optical properties of oral cancers

2023

Abstract The molecular basis of reduced autofluorescence in oral squamous cell carcinoma (OSCC) cells relative to normal cells has been speculated to be due to lower levels of free flavin adenine dinucleotide (FAD). This speculation, along with differences in the intrinsic optical properties of extracellular collagen, lies at the foundation of the design of currently-used clinical optical detection devices. Here, we report that free FAD levels may not account for differences in autofluorescence of OSCC cells, but that the differences relate to FAD as a co-factor for flavination. Autofluorescence from a 70 kDa flavoprotein, succinate dehydrogenase A (SDHA), was found to be responsible for changes in optical properties within the FAD spectral region, with lower levels of flavinated SDHA in OSCC cells. Since flavinated SDHA is required for functional complexation with succinate dehydrogenase B (SDHB), decreased SDHB levels were observed in human OSCC tissue relative to normal tissues. Accordingly, the metabolism of OSCC cells was found to be significantly altered relative to normal cells, revealing vulnerabilities for both diagnosis and targeted therapy. Optimizing non-invasive tools based on optical and metabolic signatures of cancers will enable more precise and early diagnosis leading to improved outcomes in patients.

Показать больше [+]