Cloning and expression of NS3 gene of Pakistani isolate type 2 dengue virus

Yasmin Farkhanda; Yaqub Tahir; Idrees Muhammad; Shahzad Wasim; Hashmi Abu Saeed; Aqil Kiran; Mukhtar Nadia; Zahoor Muhammad Yasir; Akhtar Naeem; Umar Sajid

Cloning and expression of NS3 gene of Pakistani isolate type 2 dengue virus

2018

Dengue is one of the major emerging viral diseases in the world, with dramatic increases in reported cases in the last few decades and annual worldwide occurrence of approximately 390 million infections. It is a highly important mosquito-vectored disease and is a problem in tropical and subtropical areas of the world. The major aim of this study was to clone and express the dengue NS3 gene, in service to its therapeutic importance for the development of stable cell lines.

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