COMPARATIVE STUDY OF THE EFFECT OF REGENERATION STIMULATION DRUG ON THE STRUCTURE AND RATE OF HAIR GROWTH IN WHITE RATS

Background. The study of the activation process of hair papilla stem cells when using regeneration stimulators to improve the structure of the hair and its growth rate is relevant both for the development of drugs for fur farming and alopecia treatment regimens. In this study, an attempt was made to stimulate stem cells in the hair papilla and improve the quality of hair. The aim of the study was a comparative study of the effect of oral and percutaneous forms of melatonin and lyotritone on changes in the growth rate and structure of hair in white laboratory rats. Materials and methods. Comparative studies of regeneration stimulation and melatonin preparations were carried out in dynamics. Experimental groups were formed to conduct the experiment: wool was removed from the animals and oral administration and coating of the shaved area of the PSR was carried out; the next group received synthetic melatonin with electromagnetic treatment similar to the previous one; the control group received distilled water. The observation was carried out for a month, then the animals were removed from the experiment in accordance with the "Rules for carrying out work using experimental animals" and in accordance with the principles of the Helsinki Declaration of the World Medical Association (Declaration of Helsinki, and approved by the Institutional Review Board). For histological studies, tissue fragments from the area with the hair removed and hair from several areas of the animal's body were used. Paraffin histological sections 5-7 microns thick were made and stained with hematoxylin and eosin. The objects of the study were microscopized at magnification x20 and x80, with the production of photographs (Vision Bio, 2014). The data obtained were processed using standard MS Excel and Statgraphics software packages. Research results. On the first day after hair removal, redness of these areas was noted, but there was no bleeding. Hair measurements carried out in dynamics showed that in the experimental groups hair growth was accelerated, and the epidermis layer was also better expressed. In the study of hair in the control group, it was found that growth was slowed down in animals that did not receive drugs, in addition, the quality of hair is worse than in the experimental groups. Conclusion. In the course of the conducted studies, it was found that the drinking of melatonin and RS by laboratory animals with percutaneous application, daily for 21 days improves the structure of the hair, its quality and accelerates growth. It was found that when the RS is soldered, the recovery time of the coat significantly decreases by a factor compared to the control group.