Harnessing the Power of Black Rice Anthocyanins: A Review in Context of Extraction and Chromatographic Techniques

Avneet Kaur; Sukhvinder Singh Purewal; Chidanandamurthy Thippeswamy Swamy; Animesh Sen

Harnessing the Power of Black Rice Anthocyanins: A Review in Context of Extraction and Chromatographic Techniques

2025

Avneet Kaur | Sukhvinder Singh Purewal | Chidanandamurthy Thippeswamy Swamy | Animesh Sen

Black rice, a well-known grain for its anthocyanin-rich pigmentation and nutritional benefits, has gained significant attention in scientific research. Anthocyanins, flavonoid pigments responsible for the distinctive coloration of black rice, offer potent antioxidant and health-promoting properties. Extraction and chromatographic techniques play pivotal roles in isolating and analyzing these valuable compounds. This review comprehensively examines the extraction methodologies, encompassing traditional solvent extraction and advanced techniques such as ultrasound-assisted extraction and supercritical fluid extraction. In addition, chromatographic techniques, including high-performance liquid chromatography (HPLC), high-speed countercurrent chromatography (HSCCC), and preparative HPLC (Prep-HPLC) are demonstrated in detail for precise identification and quantification of anthocyanins in black rice.

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