Microbial Diversity Drives Decomposition More than Advantage of Home Environment—Evidence from a Manipulation Experiment with Leaf Litter

Masoud M. Ardestani; Jaroslav Kukla; Tomáš Cajthaml; Petr Baldrian; Jan Frouz

Microbial Diversity Drives Decomposition More than Advantage of Home Environment—Evidence from a Manipulation Experiment with Leaf Litter

2025

Masoud M. Ardestani | Jaroslav Kukla | Tomáš Cajthaml | Petr Baldrian | Jan Frouz

Microbial diversity plays a crucial role in litter decomposition. However, the relationships between microbial diversity and substrate successional stage are the drivers of this decomposition. In this study, we experimentally manipulated microbial diversity and succession in post-mining soil. We used leaf litter samples from two forests of a post-mining site near Sokolov, Czech Republic: one alder plantation and one mixed forest with birch aspen and willow. Litter from each site was decomposed in the field for 3 and 12 months. The litter was X-ray sterilized and part of the litter was kept unsterilized to produce inoculum. Leaf litter samples of two different ages (3 and 12 months) from each site were each inoculated with litter of two different ages (3 and 12 months), using less and more diluted inoculum, producing two levels of microbial diversity. In each of these eight treatments, the bacterial community was then characterized by amplicon sequencing of the 16S rRNA gene and microbial respiration was used to assess the rate of decomposition. A significantly higher respiration (<i>p</i> < 0.05) was found for the litter inoculated with the higher level of microbial diversity. Higher respiration was also found for the younger litter compared to the older litter and both litter origins. This shows a reduction in microbial respiration with substrate age and inoculation diversity, suggesting that microbial diversity supports the decomposition of soil organic matter.

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