Assessment of Potential Interactions Between Phytase and Glycosidase Enzyme Supplementation on Nutrient Digestibility in Broilers

The effects of microbial 3-phytase and glycosidase enzymes, and their interactions, on energy values and nutrient digestibility in diets rich in nonstarch polysaccharides (NSP) were studied in diets based on corn, wheat, or barley. Four diets were prepared with each cereal grain. One had no enzymes, a second had 500 units of phytase, a third had glycosidase enzyme, and a fourth had phytase and glycosidase. The glycosidases used were α-galactosidase (corn diet), xylanase (wheat), and β-glucanase (barley). Glycosidase decreased intestinal viscosity, whereas phytase increased this parameter in corn diets. Phytase increased AME in corn diets, whereas β-glucanase in barley diets improved AME and AME n, and digestibility of dry matter, starch, β-glucans, and lipid. Xylanase in wheat diets improved dry matter and starch digestibility. Phytase increased total phosphorus retention in all diets, and significant interactions between glycosidase enzymes and phytase were detected in wheat and barley diets. Phytase decreased phosphorus excretion in corn and barley diets, whereas α-galactosidase increased phosphorus excretion in corn diets. Phytase in corn diets and β-glucanase in barley diets increased calcium retention, whereas inclusion of xylanase decreased calcium retention in wheat diets. Phytase and β- glucanase decreased calcium excretion in corn- and barley-based diets, respectively. An interaction was detected between phytase and β-glucanase in barley diets, in which calcium excretion was reduced. In general, no negative interactions between phytase and glycosidase enzymes were found, indicating that both types of enzymes may be used together in feeds based on corn, wheat, or barley

This work is based on research project SC-99058 supported by the Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), which also supported the fellowship of J. Juanpere. The support of Comissió Interdepartamental per a la Recerca i Tecnologia (CIRIT) is also acknowledged. The authors express their gratitude to BASF, Industrial Técnica Pecuaria S.A, Lesaffre Feed Additives, and Roche Vitamins Ltd., who supplied the enzymes

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