Integrated amplicon sequencing and transcriptomic sequencing technology reveals changes in the bacterial community and gene expression in the rhizosphere soil of Asparagus cochinchinensis

Xiaoyong Zhang; Shuai Yang; Jingsheng Yu; Xiongwei Liu; Xuebo Tang; Liuyan Wang; Jinglan Chen; Huimin Luo; Siyu Liang; Xiaoai Wang; Changmin Liu; Chi Song

Integrated amplicon sequencing and transcriptomic sequencing technology reveals changes in the bacterial community and gene expression in the rhizosphere soil of Asparagus cochinchinensis

2025

Asparagus cochinchinensis has been recognized as an edible and medicinal herb in China, which has resulted in high market demand. Therefore, optimizing its cultivation practice is essential to maintain and enhance production levels. In this study, we simultaneously employed amplicon sequencing and transcriptomic sequencing technology to analyze the rhizosphere bacterial community structure and gene expression level in A. cochinchinensis collected from the main production areas. Results showed that a total of 50 phyla, 138 classes, 343 orders, 490 families, and 875 genera were identified, of which Proteobacteria and RB41 were the most abundant at the phylum and genus level, respectively. Transcriptomics results showed that the gene expression was related to biotic stress resistance, plant growth and development, and polysaccharide metabolism, which was supported by qPCR results. These findings offer valuable insights into the utilization safety and quality improvement of A. cochinchinensis.

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