AAV-2 VECTOR INTEGRATE INTO THE OVINE MYOBLAST GENOME RANDOMLY AND PROMOTE DIFFERENTIATION AND PROLIFERATION VIA FOLLISTATIN OVER-EXPRESSION OF ERK1/2 AND AKT SIGNALING PATHWAYS
2016
Mahmood Nazari | Fatemeh Salabi | Lixin Du
The aim of our study was to investigate effect of FST over-expression by using AAVserotype 2 (AAV 2) vector on ovine primary myoblast (OPM)differentiation andproliferation. Primary myoblast cultures were obtained from 60-day-old sheep fetuses.Western blot confirmed that AAV2 could successfully express FST protein intransduced primary myoblast cells. Southern blot results demonstrated that AAVvectors integrated at apparently random genomic sites and promoted the transgenicmyoblast proliferation and differentiation. The results suggested that the AAV systemcould be used to generate transgenic meat sheep in the future
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