MOLECULAR DETECTION OF TEM GENE IN ESCHERICHIA COLI O157:H7 ISOLATED FROM CHILDREN AND BUFFALOES IN BASRAH PROVINCE
2016
Raneem A. Kareem | Bassam Y. Khudaier
During a period of five months (August 2015 to December 2015), a total of 250 samples werecollected 125 from hospitalized children suffering from diarrhea, and 125 from buffalo feces samplescollected from different regions in Basra Province, (Basra City Center, Abo alkaseeb, alqurna,karmat Ali, A lzobeer). All specimens were screened for the presence of E. coli O157H7. A total of104 (41.6%) of suspected E. coli isolates were obtained : 62 from children stool and 42 from buffalofeces, All suspected isolates were tested biochemically. 6 out of 62 from children stool 9.7% and 4out of 42 from buffalo feces 9.5% were Non-Sorbitol Fermented E. coli (NSFEC). All the isolateswere found to be resistant to at least 7 antibiotics to which they were subjected. Therefore, all thesefour isolates were considered to be multidrug resistant. PCR assay for amplification of tem generevealed that 6 of the E. coli O157:H7 isolates that isolated from children and buffalo were positivefor tem gene.
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