DETERMINATION OF FECX, FECB AND FECGH MUTATIONS IN IRANIAN ARABIC SHEEP
2016
Godratollah Mohammadi
Genetic markers are one of the best methods to evaluate animal genetics potential.FecX, FecB and FecG genes have belonged to TGFβ superfamily. These genes haveadditive effect on litter size and ovulation rate in sheep. The aim of the present study wasdetermination of FecX, FecB and FecGH mutations in Iranian Arabic sheep. In this study100 blood samples were collected of prolific sheep of Arabic breeds. DNA of bloodsamples was extracted by modified salting out method. Site of mutation was amplifiedusing specific primers and PCR products were digested with special restricted enzymesfor FecX, FecB and FecGH mutations. Results have showed no mutations of FecX, FecBand FecGH mutation were detected in Iranian Arabic sheep breed. Therefore, FecX, FecBand FecGH mutations did not cause of prolificacy in this breed and further research isrequired to evaluate the relationship of the fecundity genes with litter size and ovulationrate in this breed.
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