INVESTIGATION OF PHYLOGENIC RELATIONSHIP AMONG ESCHERICHIA COLI ISOLATED FROM CLINICAL AND SUBCLINICAL MASTITIS IN DIFFERENT ANIMALS IN BASRAH PROVINCE
2017
Ibrahim H. Madhloom | Rasha M. Othman
E.colican be assigned to one of the four main phylogenetic groups A, B1, B2 and D, which can be divided into seven and then into subgroups: A0, A1, B1, B22, B23, D1, and D2, in addition group B1can be divided into subgroups B11 and B12, using multiplex PCR according to the presence/absence or combinationof the three phylogeny genetic markers chuA, yjaA and DNA fragment TspE4.C2.In the currentstudy atotal of 30E. coli isolates were obtained from clinical and subclinical samples from mastitis in cows, sheeps and goats by standard bacteriological methods. Results found that the most isolates of E. coli belong to the phylogeny groups A and B1. Group Aincluded (14 isolates, 46.7%) belonged to subgroup A0 about (6 isolates, 20.0%), and (8 isolates, 26.7%) to A1 subgroup. On the other hand results showed group B1 composed (14 isolates, 46.7%). Group B1 can be also classified into subgroups B11 included (8 isolates, 26.7%) and B12 about (6 isolates, 20.0%). In addition our results showed (1 isolate, 3.3%), assigned to B2 belonged to subgroup B23 and (1 isolate, 3.3%), fitted in D belonged to subgroup D1. No isolates were found to belong to subgroups B22 and D2. Phylogeny pedigree was done according to the data recovered previously. This study explains that the distributions of E. coli isolates in phylogenetic groups (A, B1, B2 and D) varied depending on the climatic zone and environmental factors such as dietary, climatic conditions and geographic.
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