21- STUDY THE EFFECT OF CAFFEINE ON BODY WEIGHT GAIN AND LIPID PROFILE IN ADULT MALE RATS TREATED WITH HYDROGEN PEROXIDE.

The present study was aimed to determine the ameliorative effect of caffeine on Body weight and lipid profile in male rats treated with hydrogen peroxide (H2O2) . Seventy Two adult male rats were used in this study . The study included two experiments ,in each experiments 36 males were randomly assigned two six equal groups of six animals in each group .The animals in both experiments were treated with the same substances and doses for each group as follows .Group one (control) animals were drenched normal saline ,Group two animals were treated (5.63 mg/kg. Bw )H2O2 daily by oral gavage also group three , animals were treated with low dose caffeine (150 mg /kg Bw) daily .Group four , animals were treated with high dose caffeine (250 mg /kg Bw) daily . Group five . animals were treated (5.63 mg/kg. Bw )H2O2 dose after 1 h animals were given low dose of caffeine (150 mg/kg Bw) .Group Six animal were treated with H2O2 dose(5.63 mg/kg Bw) each rat after one hour was given high dose of caffeine (250 mg /Kg Bw) . The first experiments lasted for one month and second experiments lasted for two months . At the end of the two experiments, animals of all group were sacrificed under chloroform anesthesia .Blood samples were collected from the heart directly by cardiac puncture and the serum was separated to measure the lipid profile . The result revealed a significant decreased in body weight gain in H2O2in first and second experiments compared with control group . While a significant improvements were recorded in body weight gain in all treated groups compared with H2O2 group but still significantly lower compared with those of control group. A significant increase in TC ,TG and LDL-c were recorded in H2O2 group in both experiments compared with control group on the other hand no significant difference was recorded in HDL-c level in H2O2group in first experiments while a significant decreased was recorded in second experiments compared with control group . Finally a significant degrees of improvement were observed in lipid profile in all treated group compared with H2O2 .