INVESTIGATE THE ANTIBACTERIAL ACTIVITY OF AMYGDALIN AGAINST SOME TYPES OF ANTIBIOTIC RESISTANT PATHOGENIC BACTERIA
2018
Ahmed A. Mhawesh | Dhafar N. Al-Ugaili | Yasameen Mad olaiwy
Extraction of Amygdalin from apple seeds by alcoholic extraction procedure were doneand applied a high-performance liquid chromatographic (HPLC) procedure for quantification ofit to investigate extraction efficiency and (16) bacterial isolates ( 4 isolates of Staphylococcusaureus, 4 isolates of Escherichia coli , 4 isolates of Pseudomonas aeruginosa and 4 isolates ofStreptococcus pyogenes from skin infections samples are choose by accomplished of drugsensitivity tests as a multidrug resistant, the antimicrobial activity of methanolic extract ofAmygdalin from crushed apple seeds was tested against each group of four pathogenic bacteriaspecies of gram positive (Staphylococcus aureus and Streptococcus pyogenes ) and gramnegative (Escherichia coli and Pseudomonas aeruginosa) by well diffusion method, the resultsshown that plant extract gave antibacterial activity through the inhibition zone , but theconcentration of the raw material was more inhibited than the dilute substance concentration,inhibition of the substance was obtained on all types of bacteria were in Staphylococcus aureus,inhibition zone of substances concentrations (100 mg/ml, 50 mg/ml) were (40, 28 mm)respectively , in Pseudomonas aeruginosa inhibition zone of substances concentrations (100mg/ml, 50 mg/ml) were (50, 35 mm) respectively, in Escherichia coli, inhibition zone ofsubstances concentrations (100 mg/ml, 50 mg/ml) were (40, 25 mm) respectively, also thisresults showed that three types of bacteria above were inhibited in synergistic effect, whileinhibition zone of Streptococcus pyogenes in concentration 100 mg/ml was 30 mm and inconcentration 50 mg/ml was 20 mm.
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