SEROLOGICAL AND MOLECULAR DETECTION OF TOXOPLASMA GONDII IN MEAT AND MINCED MEAT IN BASRA CITY
2016
Battol Ali Majeed | Wamedh Hashim Abbas
The present study was conducted during the period between September 2017and March 2018 to detect Toxoplasma gondii in meat. A total number of 387 blood,meat and minced meat were collected from cattle, sheep, goat and buffalo carcasses atslaughter house and butcher markets in Basra city. Molecular and serological methodswere applied to detect the infection of T. gondii by using PCR and ELISA tests for allthese samples. The results of ELISA showed that 25.32% of samples were positive,while 15.762% were positive for PCR test. Sheep showed the highest positive resultsfor ELISA and PCR tests (33.036%, 22.321% respectively) flowed by goat (29.333%,18.667%), cattle (25.439%,14.912%) and buffalo (11.628%, 4.651%) respectively.Positive ELISA and PCR results for minced meat (37.113%, 25.773% respectively)were higher than meat from both butcher markets (27.778%, 18.519%) andslaughterhouse (17.582%, 9.89%) respectively, while the results of ELISA and PCRconducted for blood analysis were 17.582% and 6.593% respectively. Highertoxoplasmosis ratio (32.195%, 21.951%) for ELISA and PCR respectively forsamples collected from butcher markets in the present study.
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