EFFECTS OF FETAL CALF SERUM (FCS) WITH ESTRADIOL 17Β (E2) ON EMBRYO PRODUCTION IN LOCAL BUFFALOS(Bubalus bubalis)
2018
Ihsan A. H. | Alaa A. Sawad | Husamaldeen A. Al-Salim
The study was conducted to investigate the effect of add supplement (FCS andE2) on culture media (Ham’s F-10 and DMEM) on in vitro maturation , in vitrofertilization(IVF) and embryo development. This study was conducted at thelaboratories of Theriogenology, Department of Surgery and Obstetrics, College ofVeterinary Medicine, Basrah University, during the period extended from January2017 to the end of April 2018. The samples of study were female reproductive systemand male testis (150 Ovaries and 30 testes) collected from (Al-Basrah abattoir house)after slaughter at fifteen minutes. All samples were transported in sterilize and cleancool boxes at (4-8ºC) within 1-2hrs to the center research unit. Oocytes were collectedby aspiration method. Only grad A and B quality oocytes were selected and incubatedin an appropriate maturation medium (Ham’s F-10 and DMEM) at (38.5 C), 5% CO2and 95% relative humidity for 24-28 hrs. Spermatozoa were obtained by slicing ofcaudal epididymal of buffalo's bull. Sperms with matured oocytes were incubated inan appropriate maturation medium at (38.5 C), 5% CO2 and 95% relative humidityfor 16 -20 hrs. then the fertilized ova were re-incubated in fresh media with changes50% of media every day and examined every 24hrs for( 4) days to follow embryonicdevelopment The results showed:There was high significant (P<0.01) difference in the percentage of Oocytesmaturation in Ham’s F-10 and DMEM with supplement (FCS and E2) media groupscompared with control media groups. The results also showed highsignificant(P<0.01) difference in the percentage of Oocytes fertilized in Ham’s F-10 and DMEM supplement (FCS and E2) media groups compared with control mediagroups.
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