Molecular and Genetic Diversity of Ovine Hemotropic Mycoplasma in Nineveh, Iraq
2025
Salam Abd Esmaeel | Sufyan AL Shamo
Ovine hemotropic Mycoplasma (Ohm)are zoonotic pathogens that are emerging and are responsible for severe hemolytic anemia and substantial financial losses. The purpose of this study was to use the conventional polymerase chain reaction (C-PCR) method to molecularly detect Ovine hemotropic Mycoplasma spp. in infected sheep in Nineveh Province, Iraq. Additionally, the phylogenetic analysis of Ohm spp. diagnosed in this study was investigated. Sheep from various regions of Nineveh province were collected to provide a total of 241 blood samples (3 ml). Results revealed that the infection rates of Mycoplasma ovis and Candidatus Mycoplasma haematovis were 52.6% and 23.2%, respectively, using C-PCR with a primer specific to the species. Four 16S rRNA gene sequences were extracted from sheep blood for individual sequencing analysis. The NCBI GenBank contains the sequences of Ohm under the accession numbers PV273224 and PV273225 (Mycoplasma ovis) and PV273226 and PV273227 (Candidatus Mycoplasma haematovis). These sequences were identical (100%) to those associated with Mycoplasma ovis found in the following databases: NCBI GenBank (GU230142 and AF338268 in the United States), MH379799 in Brazil, MF377458 in Turkey, JF931138 in Japan, MW547439 in Poland, ON202709 in Germany, and EU828582 in Switzerland. Additionally, OQ310852 was identified in Egypt as Candidatus Mycoplasma haematovis. Mycoplasma ovis and Candidatus M. haemovis, two ovine hemotropic mycoplasmas, were first detected in sheep in the Nineveh province in this study. It was observed that they are ubiquitous, and this discovery may prove advantageous for future research and strategic management of this mycoplasma species in the study region.
Показать больше [+] Меньше [-]Ключевые слова АГРОВОК
Библиографическая информация
Эту запись предоставил Directory of Open Access Journals
