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BIOCHEMICAL CHARACTERISTIC AND ELEMENT COMPOSITION OF CICHORIUM INTYBUS, KONUS CULTIVAR
2019
N. A. Golubkina | Ju. P. Shevchenko | V. A. Kharchenko | O. V. Kosheleva | A. V. Soldatenko
Chicory is widely cultivated in many countries of the world due to high nutritional and pharmacological value. The possibility of chicory forcing in winter provides quick production of salad heads, which biochemical characteristics are not fully described. Concentration of sugars, water soluble compounds and antioxidants in roots, leaves and stumps of salad C.intybus Konus cv are studied. Element composition of roots and leaves is obtained using ICP-MS method. The highest amount of water soluble compounds and ascorbic acid was indicated in stumps, while the highest polyphenol concentration was demonstrated in leaves. Antioxidant activity of alcoholic extracts reached maximum in leaves and did not differ between roots and stumps. The ability of Konus variety to hyperaccumulate selenium was revealed for the first time: selenium concentration reached 2800 mcg/kg d.w. in roots and 3800 mcg/kg d.w. in leaves. Konus cv was characterized also by high accumulation of chromium, manganese, molybdenum and iron. Uneven distribution of elements between roots and leaves are demonstrated for all elements except Al, As and Si. Intensive root-leaves transport was revealed for Ca, Cr, Cu, Fe, K, Mg, Mn, Mo, Ni, P, Pb, Se and Zn and decreased rootleaves flow for B, Cd, Co, Li, Sn, Sr, V and especially Na. One hundred g of fresh leaves provides 22% of the daily adequate consumption levels for Se, 91%- for Cr, 15%- for Mo, 14%- for Fe and 12 % for Mn. The results suppose high prospects of C.intybus Konus cv as a functional food, capable to optimize the human Se and Cr status.
Показать больше [+] Меньше [-]Lactic acid bacteria, creating the optimal starting conditions for fermentation of cabbage
2019
Nataliya E. Posokina | Anna I. Zakharova
Relevance Fermentation of vegetables is usually carried out in the traditional way (spontaneous fermentation using native microflora), but the quality of the finished product is difficult to predict. Very often, due to the low initial amount of lactic acid bacteria or their low activity, the result of the process remains unpredictable, which can lead to the loss of a significant amount of product. In the fermentation of vegetables involved several types of facultatively anaerobic lactic acid bacteria. In order to control the fermentation process and make it directed, it is necessary to study which lactic acid bacteria are involved in the fermentation process, the period in which their growth and death, and how it affects the organoleptic properties of the finished product, as well as to study the activity of lactic acid microorganisms in the fermentation process. When fermentation of vegetables are not only the original nutrients such as vitamin C, amino acids, dietary fibers, etc., but also develop functional microorganisms such as lactic acid bacteria. Fermentation has an important effect on the quality and taste, so it is very important to study the fermentation process, microbial diversity and changes in nutrients and chemical elements in the fermentation process. Reducing the rate or preventing microbial spoilage of food is based on four main principles: minimization of product contamination by microorganisms; suppression of growth and reproduction of microorganisms-contaminants; destruction of microorganisms-contaminants; removal of microorganisms-contaminants. Fermentation is based on a combination of the first three principles and is achieved by creating conditions for the growth of specific microorganisms that can give food the desired taste, aroma, texture and appearance. Results This review is devoted to the scientific aspects of vegetable fermentation, including crops that contribute to the creation of optimal conditions for the development of the main pool of lactic acid microorganisms, the production of finished products of high quality and the prevention of microbial spoilage. It is shown that at the first stage of fermentation lactobacilli of the genus L. mesenteroides play a determining role. It is their "work" to create optimal conditions for the development of the target lactic microflora depends on the quality of the finished product. This fact should be taken into account when creating industrial bacterial starter cultures – "starter cultures" for the directed process of fermentation of vegetables.
Показать больше [+] Меньше [-]The allocation of carbohydrates from vegetable raw materials and their identification with the use of capillary electrophoresis
2019
Stanislav I. Biryulin | Nataliya E. Posokina | Marina V. Trishkaneva
Relevance. Carbohydrates are integral components of the cells and tissues of all living organisms of the plant and animal world; they are important components of the plant cell walls, as well as the extracellular matrix of animal and human tissues. The type of monosaccharide composition of polysaccharides determines their biological functions for the organism. However, it is difficult to establish the relationship between the structure of the carbohydrate molecule and its biological function.Methods. In this article, the author provides an overview of methods for identifying the monosaccharide composition of plant polymers by capillary electrophoresis, in order to optimize the sample preparation procedure and the conditions of analysis. A scheme for the stepwise release of polysaccharides from raw materials is given: the isolation of soluble monosaccharides and polysaccharides, the extraction of the pectin fraction, the extraction of cellulose and hemicelluloses, in order to study the composition of each fraction. A procedure for acid hydrolysis of polymers to monosaccharides using oxidizing agents such as sulfuric and trifluoroacetic acid is described. In the final part of the article, three different schemes for the identification of the monosaccharide composition were analyzed, namely, separation of the components under strongly alkaline conditions, preliminary derivatization with 1-phenyl-3-methyl-5-pyrazolone, preliminary preparation of (S) (-) α-methylbenzylamine S-PEA) derivatives.Results or Findings. An analysis of the literature shows that the composition of polysaccharides is widely studied by various methods. At the same time, a number of problems remain associated with the implementation of these methods in terms of sample preparation and identification of all monosaccharides characteristic of the studied raw materials. The method of capillary electrophoresis can solve some of these problems; however, little has been studied. The study of carbohydrates in plant objects begins with the procedure for extracting carbohydrates (polysaccharides and monosaccharides) from the plant matrix, hydrolysis of polysaccharides, and subsequent identification using the CE method. The optimization of this scheme of the CE method for determining the composition of polysaccharides is an urgent scientific task.
Показать больше [+] Меньше [-]Effectiveness of complex application of organomineral fertilizer EcoFus with the growth regulator Zircon on sage (Salvia officinalis L.)
2019
N. I. Kovalev | E. L. Malankina
Relevance. Meeting the needs of the pharmaceutical industry with essential oil raw materials and natural essential oils is relevant.Methods. The aim of the study is to determine the effectiveness the complex application by organomineral fertilizer EcoFys and the growth regulator Zircon on sage (Salvia officinalis L.) in conditions of Non-chernozem zone of the Russian Federation. The experimental part of the work was performed in Institute of Medicinal and Aromatic Plants in 2014-2015 by field experiments.Results. It has been established, that integrated application EcoFys and Zircon can increase yield of culture. In addition, it is shown that contain of essential oil in medicinal raw don’t increase and raising collection of essential oil provides only due to higher yield of herb.
Показать больше [+] Меньше [-]Testing of tomato hybrids of the breeding of the company "Gavrish" in JSC "Teplichnoye" of the Tambov region
2019
Konstantin A. Chupkin | Vera I. Terekhova | Anastasiya V. Konstantinovich
Relevance Currently, the requirements for modern tomato hybrids are increasing both from consumers and from producers. Modern tomato hybrids in extended circulation should yield at least 60 kg / m2, be distinguished by high quality of fruits, early ripeness, possess manufacturability, resistance to major diseases. Along with the traditional form of fruits, hybrids with original shape, color, taste and aroma are interesting for manufacturers. Methods The aim of the research was the variety study of indeterminant F1 hybrids of tomato breeding of the company "Gavrish" in JSC "Teplichnoye" of the Tambov region. Studies were conducted in 2017-2018 in the extended and summer-autumn turnover in the conditions of JSC "Teplichnoye" of the Tambov region in accordance with generally accepted recommendations for research with vegetable crops in greenhouses. The objects of the study in extended circulation were tomato hybrids: F1 Bao Bab, F1 Baloven, the F1 Torero hybrid was taken as a control. In the summerautumn turnover, a study was made of the rose hybrid – F1 Panthera; the Rosario F1 hybrid was used as a control; a hybrid with a plum-shaped fruit – F1 Armata, control – a hybrid F1 Lezghinka. Results According to the results of the study of tomato hybrids, the selection of the Gavrish company at the enterprise made the decision to grow it in extended circulation and increase the area under F1 Baloven, in the summer-autumn turnover under the F1 Panthera hybrid.
Показать больше [+] Меньше [-]USING INDICATORS OF THE ESSENTIAL OILS COMPOSITION TO IDENTIFY THE VARIETY
2019
T. V. Sachyuka | N. A. Kovalenko | G. N. Supichenko | V. N. Bosak
Essential oil crops and their essential oils are widely used in perfumery, cosmetic and food industries, traditional and folk medicine. The most important qualitative characteristic of essential oil crops is the content, component and enantiomeric composition of essential oils. Basil (Ocimum basilicum L. and Ocimum tenuiflorum L.) and hyssop (Hyssopus officinalis L.) belong to the promising essential oil crops for cultivation in the soil and climatic conditions of the Republic of Belarus. In the Belarusian State Agricultural Academy has created a number of varieties of essential oil crops, including 3 varieties of basil Ocimum basilicum L., 1 variety of basil Ocimum tenuiflorum L.) and 1 variety of hyssop Hyssopus officinalis L. The new varieties of basil and hyssop are characterized by a complex of morphometric, morphological and phenological sings.In the studies of the Belarusian State Agricultural Academy and the Belarusian State Technological University, the yield and content of essential oils of new zoned varieties of basil and common hyssop were analyzed. Using the method of enantioselective gas chromatography, the component and enantiomeric composition of essential oils of the studied varieties was determined. As a result of the research, it was determined that each variety has its own characteristic component and enantiomeric composition of essential oils. The studied varieties of basil and common hyssop contained up to 20 and 18 different components of essential oils respectively. The features of the component and enantiomeric composition of essential oils allow identifying the already created varieties of essential oil crops, as well as carrying out their selection to create varieties with certain properties.
Показать больше [+] Меньше [-]Production of doubled haplois in Brassica purpuraria
2019
E. V. Kozar | K. S. Korottseva | O. V. Romanova | O. A. Chichvarina | L. Yu. Kan | V. A. Ahramenko | E. A. Domblides
Relevance. In recent years vegetable crop Brassica rapa ssp. chinensis var. purpuraria (synonyms: Brassica campestris L. var.purpurea Bailey; Brassica rapa L. ssp. chinensis var. purpurea) is gaining popularity as an object of genetic and molecular researches, and as an economically valuable vegetable plant due to the high content of biologically active compounds and distinctive economically valuable traits. Effective technology for development DH-plants to accelerate the breeding process for this culture has not been developed yet, so research in this area is relevant.Materials and methods. The study included two varieties from the collection of Vavilov AllRussian Research Institute of Plant Industry (VIR): No. 1301 (China) and No. 1357(Netherlands). Both protocols standard unmodified and with addition of silver nitrate (AgNO3) in the medium for embryogenesis induction were used in experiments for production of DH plants from isolated microspore in vitro. Direct chromosome counting in meristem cells and flow cytometry were used to determine the ploidy of regenerating plants.Results. As a result of the study embryogenesis in B. purpuraria culture can develop with the use of a standard protocol as well as with the addition of silver nitrate that showed a positive effect on the induction of embryogenesis. The yield of embryoids varied depending on the genotype of the individual plant within the variety accession. The highest yield of embryoids was 40 embryoids/petri dish. The main problem at the stage of regeneration is that about half of the regenerating plants occurred to be albinos and were not viable. We show a high degree of spontaneous chromosome doubling in regenerated plants (all analyzed plants were doubled haploids). In total 38 regenerated plants were obtained from accession No. 1301. It was shown that four DH-plants had self-incompatibility after self-pollination, but seed progeny from other plants was obtained. The created material was taken for genetics study and breeding work.
Показать больше [+] Меньше [-]EVALUATION OF THE GARDEN CARROT ACCESSIONS FOR THE POPULATION OF <i>CAVARIELLA AEGOPODII</i> (SCOP.) AND ECONOMICALLY VALUABLE FEATURES OF CULTURE IN THE CONDITIONS OF THE BRYANSK REGION
2019
I. V. Sycheva | A. V. Soldatenko | S. M. Sychev | A. S. Pankrushova
The article presents the results of research on the biology of willow-carrot aphid and evaluation of varieties and hybrids of garden carrot in the Bryansk region. The aim of scientific research was to study the biological characteristics of willow-carrot aphid - Cavariella aegopodii (Scop.) (Homoptera: Aphididae), the influence of weather and climatic conditions on the pest prevalence, the degree of plant population, as well as the evaluation of varieties and hybrids of garden carrot on the complex of economically valuable traits. It was established that the average daily temperature of 18°C and above contributed to the intensive pest infestation of carrot plants in the first decade of July. The peak of the number of willow-carrot aphids was noted in I-II decades of July. With the increase of temperature increased the fecundity of females of the aphid up to 60-70 larvae. In 2017-2018 detected relatively resistant varieties and hybrids of garden carrot – Minor, Marlinka, Nantskaya 4, Nadezhda F1with an average damage score of 1.0-1.17. The variety Shantena royal is marked with plants with an average score of 2.43, which characterizes the average resistance of the variety to damage. In the Bryansk region on the basis of "mass of roots" on average, over two years of research significantly exceeded the standard (variety Nante 4) Shantena 2461, Shantena Royal, Minor, Nante, Cupar F1, Nadezhda F1, Mars F1 by 6.2 - 37.1 per cent. On the basis of "marketable yield" varieties and hybrids of Mars F1, Cupar F1, Nadezhda F1, Shantena royal, Nante, Minor were distinguished. According to the results of biochemical analysis, a variety of Nante with a high content of carotene (185.1 mg/kg), dry matter (13.3%) and low nitrate accumulation (110.2 mg/kg) was isolated. During two years of research in the conditions of the Bryansk region, it was established that Minor, Nadezhda F1 combined relative resistance to willow-carrot aphids damage and were distinguished on the basis of “commercial yield”.
Показать больше [+] Меньше [-]Bioenergetic efficiency of cultivation of watermelon depending on agricultural practices
2019
Tatyana G. Koleboshina | Elena A. Varivoda | Olga G. Verbitskaya
Relevance The relevance of the bioenergy assessment follows from the requirements of modern production: energy saving per unit of output. Methods The article presents the data of bioenergy evaluation of the use of table predecessors, types of fertilizers and growth regulators in the technology of watermelon cultivation. Results The results of studies have shown that from the energy point of view, the proposed methods of cultivation of watermelon, are effective, because the coefficient of energy efficiency in all the studied variants is more than one. Studies have revealed the advantage of a layer of perennial grasses as a precursor to watermelon: CEE – 2.55 at its values according to the predecessor of winter rye – 1.70. The use of mineral fertilizers dose N60P90K60 in rain conditions leads to a significant increase in energy consumption of commercial products, 1.7 times more in the formation of perennial grasses and 1.3 times more than the predecessor of winter rye. A decrease in the CEE and an increase in the cost of total energy and energy intensity of 1 ton of products using manure 27 t / ha in relation to mineral fertilizers. Comparative energy analysis showed high efficiency of using water-soluble fertilizers and growth regulators for soaking seeds and processing of vegetating plants in watermelon cultivation. The use of these agricultural practices can reduce the energy costs of total energy by 70-79% compared with the introduction of mineral fertilizers dose N60P90K60, with an increase in the energy efficiency coefficient from 1.54 to 2.68-2.77. As shown by calculations from the energy point of view, the best was the reception of cultivation of watermelon table using for seed soaking and processing plants in vegetation water-soluble fertilizer master special with the addition of growth regulator megapol, the value of the EPS was amounted to 2.99, which is 7.9% and 11.5% more in comparison with potassium Humate and Ribav-extra, respectively.
Показать больше [+] Меньше [-]VEGETABLE PEA BREEDING ON TECHNOLOGY
2019
I. P. Kotlyar | V. A. Ushakov | I. M. Kaygorodova | E. P. Pronina
Breeding of high-tech varieties of vegetable peas is a priority ефыл selection at the present stage. The main requirement of processing enterprises is full and timely loading of production lines with high-quality raw materials. Peas should be aligned in size, of intense green color with high biochemical parameters. Therefore, the newly created varieties should combine a number of characteristics: high and stable yields, simultaneous ripening, resistance of the stem to lodging, resistance to most common diseases. The combination of these features allows to realize the potential of the culture fully, and to find wide application in production for created varieties. Since the 80s of the last century, FSBSI «FSVC» (Moscow region) has been actively working to increase the suitability of vegetable pea varieties for mechanized harvesting. Since these years, a number of varieties with strong shortened internodes and stem height not more than 80-90 cm have been created. Reducing plant height has improved the manufacturability of vegetable pea varieties, the stems resistance to lodging during the technical stage of ripeness has been significantly increased. However, in the biological stage of ripeness, the stem degree of lodging has been preserved, which creates additional difficulties in the seed production of vegetable peas. Since 2008, the direction of breeding has been adjusted to improve the stems resistance to lodging in combination with other economically significant traits (determinant type of growth, mustache type of leaf, green color of peas, duration of the technical stage of ripeness). As a result of this work, new varieties Cruiser, Viking, Triumph, Corsair, Barin, Hercules, Egorka have been created. The work is to introduce these varieties into production is being carried.
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