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Dissipation of Glyphosate and Glufosinate Ammonium in Soil and Weed Control in Citrus Orchards
2021
Amira Othman | Mohammed El-Zemaity | Walaa El-Sayed | Ahmad Hanafi | Khaled Ramadan
Glyphosate (GLY) and glufosinate-ammonium (GLUA) are broad-spectrum, non-selective, contact herbicides that are commonly used in fruit farms Achieving the separation and detection of glyphosate and glufosinate from soil samples by chromatography is a challenging task as they are ionic and highly water-soluble compounds. The aim of this study is conducted to determine the dissipation of GLY and GLUA applied at two dose levels in three-depth soils of orange orchards. The residues of GLY and GLUA were determined by the HPLC-UV detector. The residual detection limits of GLY and GLUA of the method were 0.03 and 0.05 ng/g in soil respectively. The obtained data indicated that GLY persistent in the soil is very short, only for 7 days, following applications of 1 to 2 kg/fed in the orange crop. GLUA dissipated in soil within 14 days of application, regardless of dose. The half-life (T/2) of GLY and GLUA were 1.68 and 1.42 days at 0 cm depth, respectively. There was no significant difference between the half-life of the two herbicides in soil at three depths. These results showed that GLY dissipation occurs rapidly in soil. However, GLUA was moderately persistent in soil. The two compounds tested showed a reduction of dry weight for four types of weeds after 14 days of recommended and double-rate application.
Показать больше [+] Меньше [-]Producing of Biodegradable Plastic Films from Un-marketable Potato Tubers
2021
Aya Kobash | Moubark Moustafa | Fathi Abdel-hadi | Ashrf Anwer
This study was conducted to reduce the volume of solid wastes by using unmarketable potato tubers for producing biodegradable plastic films to substitute oil-based plastics “OBP” which is often not biodegradable. Starch-based plastic films “SBP” were produced with different concentrations of glycerol (5, 10,15, 20,25, 30, 99.5%v/v) as a plasticizer. The physical properties of SBP including thickness, density, water absorption, and biodegradability, were studied, as well as mechanical properties including tensile strength, modulus of elasticity, elongation % and the required energy for the firm were also investigated compared with OBP samples. The results showed that the maximum value of extracted starch was 13.5% obtained by blending potato tubers. SBP films with elevated glycerol concentration in-creased the sample thickness but decreased its density. Glycerol 20% gave the best flexible compact structure SBP films. The average thickness and density of SBP film (20% glycerol) were 0.25 mm and 80.11 Kg/m3 respectively. Meanwhile, the average thickness and density for OBP films were 0.41 mm and 24.39 Kg/m3 respectively. The rate of mass loss of SBP films with 20% glycerol concentration was 72% after 96 days while the rate of mass loss for OBP films is neglectable. Consumed energy for the firm was 44.05 and 31.06 N. mm.
Показать больше [+] Меньше [-]Promoting of Abiotic Stress–Induced Resistance Using Poly-β- Hydroxybutyrate (PHB) By Rhizobium phaseoli In Common Bean Plants
2021
Shawky Selim | M. ElHaddad | Mahmoud Nassef | Wafaa Radwan | Samah Abu-Hussien
In total, 50 Rhizobium isolates were isolated from the mature root nodules of common beans plants (Phaseolus vulgaris) grown in different nine governorates of Egypt. PHB was optimized by the identified strain using response surface methodology. A total of 11 parameters (pH, incubation period, inoculum size, temperature, agitation speed, mannitol, sucrose, yeast extract, glycine, K2HPO4, and MgSO4) were analyzed for their significant effects on PHB production by the Plackett–Burman design (PBD). Sucrose, yeast extract, glycine, and MgSO4 were the main significant factors affecting PHB accumulation. Central composite design (CCD) of the response surface methodology was used to determine the optimum levels of the selected factors. Rhizobium phaseoli reached the maximum production (4.997 g/L) at run 36 in the presence of 25 g/L of sucrose, 0.0 g/L of yeast extract, 0.87 g/L of glycine, 0.3 g/L of MgSO4, and 5% of inoculation size. In vitro experiments were carried out to test the effect of different stress conditions (pH: 6–11, temperature: 5°C–50°C, salinity: 0.01%–7%, and drought: 0%–5% w/v) on the growth of Rhizobium phaseoli. The results showed that Rhizobium phaseoli can withstand 3% –5% NaCl, high temperature of 30°C– 45°C, alkalinity at pH value of 8 – 10, and drought stress at 3% – 5% w/v polyethylene glycol with growth loss of 50% when grown on modified medium and 75% when grown on the basal one. In vivo experiments were done to study the effect of drought stress levels on the growth parameters of common bean plants. In general, all the treatments with Rhizobium phaseoli grown on the modified medium were superior to Rhizobium phaseoli grown on the basal medium. Also, they showed high tolerance of drought conditions.
Показать больше [+] Меньше [-]Design of Sprinkler Irrigation Systems Using an Expert System Program
2021
Mohamed Alashram | Khaled elbagoury | Said Shaaban | Ahmed abdel-aziz
In this research, a rule-based expert system named EFSIS-ES (Engineering Factors of Sprinkler Irrigation System -Expert System) had been built, verified and validated. Therefore, the aims of this research were to build, verify and validate an expert system for making a decision for the normal user to maximize system efficiency of sprinkler irrigation. The inputs data included location data, climate data, pump data, crop data, soil data and sprinkler system data. The outputs of the expert system were number of the sprinklers on a lateral, number of laterals in the irrigation system, length of each lateral, main line length, irrigation frequency, required pump power, total dynamic head (TDH) and pump discharge output. Results showed high correlation (R2 = 0.9927) between the EFSIS-ES program and the designed spreadsheet in regard to irrigation frequency. It could be indicated high correlation (R2 = 0.9999) between the EFSIS-ES program and the designed spreadsheet regarding pump power. The regression coefficient (R2) was 0.9983 between EFSIS-ES program and SSSDPS Expert regarding total dynamic head (TDH). It could be indicated high correlation (R2 = 0.9979) between EFSIS-ES program and SSSDPS Expert regarding pump discharge output.
Показать больше [+] Меньше [-]Improvement of Irrigation Efficiency by Developing Surface Irrigation System
2021
Marwa Mohamed | Mahmoud Hegazi | essam wasif | Osama Ahmed Bedir
The experiments were Carried out in Biahmu Village at Al-Fayoum Governorate, Egypt during the two winter seasons of 2018/2019 and 2019/2020, on wheat crop to (cv. Gemmiza 11) evaluate the traditional surface irrigation system "TSIS" and the improved surface irrigation system "ISIS"(terrace), under laser leveling with two slops (S1≈ zero% and S2 ≈ 3%). The evaluation parameters included; reduction in land loss "LL", the roughness coefficient of marwa-walls, water efficiencies (i.e., water conveyance efficiencies "WCE", water application efficiencies "WAE" and water storage efficiencies "WSE"), yield productivity "Yp", total applied water and water required, irrigation water productivity "IWP" and times of water advance, recession and opportunity. The results concluded that improving traditional marwa, led to reduction in land loss by 49.46% from the area of marwa, and "WCE" increased by 83.17% in improved Marwa "IM" the compare to Traditional Marwa "TM. It was observed that on the efficiency of water added to the field irrigated by "TM". The highest level of water productivity (1.79kg wheat/m3 water) was achieved in "IM" at a level of 3%. Advanced time "T.Adv" (min) decreased at 50, 50.77, 27.6, 20.75, and 17.17% and 30.9, 40.51, 22.8, 10.89 and7.05% for "TL and IL" by sloping to "S2".
Показать больше [+] Меньше [-]Assessing Inter and Intra Molecular Genetic Variation of Litter Size in Three Egyptian Goat Breeds Using COI and F-AFLP Markers
2021
Mohamed Reda Anous | Emanuel Kodit | Hagar Elhifnawy | Mohamed Rashed | Mohamed Sadek
establishing adequate utilization of genetic variation in developing livestock breeding programs. Cytochrome oxidase subunit I (COI) gene technique was used in the present study to identify and differentiate the main three Egyptian local goat breeds (i.e. Baladi, Zaraibi and Barki) via DNA barcoding to confirm its species identity and provide valuable DNA sequence source in the nucleotide online database for further studies. Blast (Basic Local Alignment Search Tool) results confirmed samples to be Capra hircus (100%) with no variation among the studied breeds. On the other hand, the Fluorescently Amplified Fragment Length Polymorphism (F-AFLP) technique was applied to assess genetic variation among and within the three breeds for litter size character. F-AFLP analysis of triplicates per breed produced 164 polymorphic loci. At the same time fixed and private bands varied among the three breeds; 47, 17 and 14 bands and 9, 19 and 27 bands for Baladi, Zaraibi and Barki, respectively. Analysis of Molecular Variance (AMOVA) showed 3.8% and 96.1% variance among and within breeds, respectively. Population re-allocation showed that all samples of Baladi breed are outliers, Zaraibi breed one outlier and two hybrids and in Barki breed one hybrid, one outlier and one allocates itself. Private bands in excel filter (using virtual inspection in excel) showed fixed bands of 213bp molecular weight at locus 35 in both Baladi and Zaraibi breeds. These bands considered as genetic marker for litter size trait (i.e. high prolific animals).
Показать больше [+] Меньше [-]Improvement of Fungal Phytase Production and Its In vitro Application in Ruminant Nutrition
2021
NOHA HASSAAN | Abd El-Nasser khattab | M. Khorshed | N. El-Bordeny | A. Abedo | M. Shoukry
Various fungal genotypes (Aspergillus niger NRRL 3135 (AN1), Aspergillus niger NRRL 326 (AN26), Aspergillus terrus F2-Kh (AT) and Mucor racemosus NRRL 3639 (MI)) were studied for their ability to produce phytase and improve the produced enzyme by ethyl-methane sulfonate (EMS) mutagenesis. AN1 showed the highest phytase activity on phytase screening medium supplemented with glucose (PSMG) after 8 days of incubation (reached 1875.40 IU/mL). The phytase activity of AN1 increased with increasing incubation time and the highest value was achieved at 12 days of incubation (2859.33 IU/mL). The exposure of AN1 spore suspension to 200 mM of EMS for different times enhanced the phytase activity and that mutant 20 Mn exhibited the highest phytase activity (reached 4520.5 IU/mL) therefore it was chosen for the next experiment. An in-vitro gas production procedure was carried out to evaluate the impact of using various amounts of laboratory produced phytase (PE) compared with commercial phytase (Axtra® PHY) on nutrients availability of ruminant's ration. Six levels (0, 400, 800, 1200, 1600 and 2000 IU phytase-1Kg dry matter) of phytase enzyme were evaluated with tested ration consisted of 40% berseem hay (BH) and 60% concentrate feed mixture (CFM). In-vitro dry and organic matter degradability (IVDMD and IVOMD), total gas production (GP), short chain fatty acids (SCFA’s) and inorganic phosphorus (Pi) concentration were improved significantly (P<0.05) by phytase addition from the two sources of phytase and the highest significant (P<0.05) values achieved at the level of 1200 IU. Results suggest that phytase activity was influenced by exposure to EMS mutagen compared to the wild type. Also, the produced enzyme source has the ability to improve the utilization efficiency of phytate diets as evidenced by the significant (P<0.05) increase in all tested parameters compared to the commercial source.
Показать больше [+] Меньше [-]Natural Pigments Production by Local Bacterial Isolates for Use as Antibacterial and Antioxidant
2021
Sohila El-Sayed | El-Sayed Saleh | Khadiga Aboutaleb
Among 46 local bacterial isolates capable synthetized pigments, 26% produced extracellular of blue green and fluorescent whereas 74% produced intra-cellular pigments of red, brown, yellow, orange, and rose. Seven isolates were selected as a potential candidate for the synthesis of pigments. They were Pseudomonas fluoresces, P. aeruginosa, Serratia marcescens, Azotobacter chroococcum, Micrococcus kristinae, M. luteus, and M. roseus after their morphological and biochemical studies. They capable to produce fluorescent, blue green, red, brown, orange, yellow, and rose with maximum absorbance at 400, 520, 530, 320, 440, 460, and 470 nm, with an optical density of 0.62, 1.42,1.35, 1.11, 0.45, 0.98, and 0.40, respectively. Antibacterial activity of pigments was studied against 7 bacterial pathogenic strains namely P. aeruginosa ATCC27853, Escherichia coli 0157h7 ATCC25922, Klebsiella pneumonia ATCC00607, Salmonella typhimurium ATCC25566, Listeria monocytogenes ATCC19115, Staphylococcus aureus ATCC29737, and Bacillus cereus ATCC33018. The last tested strain behaved high significant (p≤ 0.05) sensitivity for blue green, fluorescent, brown, and rose pigments while, L. monocytogenes ATCC19115 was more susceptible for red, and yellow pigments and S. aureus ATCC29737 had more inhibited by orange pigment. These bacterial pigments seemed to have antioxidant activity which inhibited the formation of diphenyl-2-picrylhydrazyl radicals with percentage ranged from 40 % to 80 %. It could therefore be stated that the bacterial pigments tested had a broad impact on foodborne pathogens and had an antioxidant effect
Показать больше [+] Меньше [-]Impact of Organic Fertilizers Derived from Banana and Orange Peels on Tomato plant Quality
2021
Mona Nossier
The main objective of this research is to promote the reuse of wastes resulting from different manufacturing processes such as fruit peels (e.g. banana and orange peels) as substitutes (at least partially) of mineral fertilizers. This might have positive effects in maintaining human health and reducing environmental pollution. Moreover, to compare the use of these wastes with commercial organic fertilizer i.e., compost made by Nile Company to meet the needs of tomato plants from nitrogen (N) and potassium (K) elements. To achieve the goal, two experiments were conducted. The first was an incubation experiment, which was designed to compare the organic fertilizer compost of Nile Company and the powdered banana and orange peels. The second was a field experiment achieved at the Faculty of Agriculture, Ain Shams University. Obtained results of the incubation experiment showed increases in nitrogen content by 16 to 31% and potassium by 12 to 24% due to using grinded banana and orange peels as compared to the compost treatment. Moreover, in the field experiment the concentration of nitrogen (N) and potassium (K) at different plant growth stages whether in soil or plant did not differ significantly between soil plots fertilized with mineral fertilization and those fertilized with banana and orange ones. On contrary, potassium concentration was found higher in the soil fertilized with organic fertilization than that fertilized with mineral fertilization. It has also been found to us that there are no clear significant differences between tomato fruits resulting from mineral fertilization of the ground and those that were fertilized by the mixture of banana and orange peels in terms of the ratio of nitrogen and potassium, weight, size and density of the fruit and other characteristics except for the total salts percentage, which was less Fruits fertilized with a mixture of banana and orange peels
Показать больше [+] Меньше [-]Obesity Assessments of Housewives in Great Cairo, Egypt
2021
Asmaa Abdel-Rahman | Ramadan Mahmoud | Mohamed Khallaf | Doaa H. El Sabakhawi
A cross sectional study was conducted to evaluate obesity of housewives. The Sample that comprised of 200 females was selected from different regions of great Cairo, Egypt. Age was affected the BMI of various investigated obesity classes with a high correlation. A similar finding was also detected in case of education status where such parameter was also greatly affected the BMI status. The occupation, marital status and family size did not show any significant effect in BMI. The Income parameter didn't show any significant with the BMI. So, it could be concluded that only age and education status are greatly affected the housewives obesity in the investigated sample. Life style, i.e the sports practice. The type of sport, the time spent for sport practice, the place sports practice, household works making by herself, the number of times household works practice/week, the time of sleep in day and the time of last meal before sleeping were statistically significant with the BMI. Anthropometric measurements (waist circumference, waist-to-height ratio and central obesity) are greatly affecting the BMI meaning that there is a significant difference among various investigated obesity classes owing to such anthropometric factors. Regarding to the height parameter, no significant difference was found between overweight as well as obesity class I each other, and similar finding was also found between obesity class 11as well as obesity class III. The second variable (i.e., IBW%) had no significant effect among the three obesity classes (I, II and III), while the overweight group was significantly differed rather than that of others. Considering the Central obesity variable, statistical analyses proved that no significance was detected with overweight or obesity class II. group. Similar trend was also, noticed in case of (hip circumference) in relative to obesity class I and obesity class II.
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