The present study was carried out to verify the variation of short fiber content (SFC) and its importance. The instruments and/ or methods used to measure short fiber include High Volume Instrument (HVI), Fibrograph 630 and Suter-Webb array. Materials used in this study included the extra-long and long staple varieties with wide range of lint grades. The three measures of short fiber especially, Suter-Webb-SFC exhibited high variations for lint grades and low variations for cotton varieties. Suter-Webb short fiber shows higher values than the HVI and Fibrogragh short fiber. Results indicate that most of the fiber and yarn quality properties are strongly correlated with each of the three measures of short fiber content. Comparisons between the three measures indicate that they correlate well with each other. Highly significant regression model was developed to predict short fiber content from maturity percentage, fiber length, length uniformity and fiber strength. The closer normalized short fiber content (NSF) is to 1 (or 100%), the higher the agreement between SFC and predicted short fiber content (PSF).