Cucumber production exposed to huge losses in Egypt and worldwide due to infection with Cucumber mosaic Cucumovirus (CMV). The current study focus on the impact of two Egyptian CMV isolates on metabolic and oxidative activities in cucumber plants. Two CMV isolates was obtained from cucurbitaceous crops and confirmed by DASELISA. Inoculated cucumber plants by infectious crude sap with two CMV isolates showed difference in external symptoms. The metabolic and biochemical components of cucumber plants were affected with two CMV isolates. The present study provided that, CMV infection caused a significant reduction in both photopigments and biochemical components while bioactive components (proline and phenol) and oxidative enzymes (POX, SOD, PPO and CAT) were increased significantly compared healthy ones. It was found that cucumberCMV isolate was more effective in metabolic and biochemical than Squash-CMV isolate.