Growing wastewater microalgae contrib-utes to the elimination of nutrients present in wastewater because they need N and P for the synthesis of proteins, nucleic acid, and phos-pholipids. The most sustainable approach for achieving high biomass and high lipid build-up, along with environmental protection. In the current research, nine microalgae were culti-vated on two variations of wastewater (agricul-tural and industrial dyes) and compared to the synthetic medium. The results indicated that after three weeks of the incubation period, the ability of microalgae to grow in sterilized (syn-thetic medium and wastewater) and gave greater biomass and chlorophyll (a) than in non-sterilized ones. Out of the tested nine mi-croalgae, four microalgae (Anabaena oryzae, Spirulina platensis, Anabaena sp (2) and Nos-toc sp) were selected which gave the highest significant values of dry weight, biomass productivity and chlorophyll (a) content. The selected microalgae showed the highest signif-icant values of the chemical composition, i.e., total carbohydrate, protein and lipid when cul-tivated in agricultural wastewater more than those cultivated in both industrial dye’s wastewater and synthetic broth medium. Among four microalgae, two microalgae of A. oryzae HSSASE6 (KT277789), and S. platen-sis NIES-39 (A00800) were chosen where the chemical composition contents ranged from 1.17 to 1.21-fold and 1.03 to 1.06-fold of car-bohydrate, 1.06 to 1.09-fold and 1.88 to 1.93-fold of protein and 1.41 to 1.52-fold and 1.76 to 1.90-fold of lipid more than those of other microalgae, respectively. The agriculture wastewater was inoculated with a single cul-ture of A. oryzae HSSASE6 (KT277789) or S. platensis NIES-39 (A00800) individually with 10 % of inoculum size, which more preferred than was inoculated with consortia culture. Re-sults also showed that A. oryzae HSSASE6 (KT277789) was more efficient strain for giv-ing biomass and productivity in agricultural wastewater than S. platensis NIES-39 (A00800) (about 28% more).

A field experiment was conducted in sandy soil at Ismailia Agricultural Research Station, (Ismailia Governorate, Egypt, in two successive winter seasons of 2015/2016 and 2016/2017. The experiment aimed to study the response of lupine plants (Lupinus albus L.) variety Giza 2 to cyanobacteria applied with different methods under different nitrogen fertilizer rates and inoculated with rhizobia. Results revealed that application of cyanobacteria generally increased the mean values of nodules number, dry weight of nodules and shoot dry weight of lupine plants, nitrogen uptake, biological activity of the soil rhizosphere lupine plants, yield, yield components and seed protein content as compared to those recorded by the control treatment without cyanobacteria. Increasing nitrogen rates decreased the mean values of both nodules number and dry weight of nodules. On the contrary, increasing nitrogen rate up to 100% of the recommended one increased all the other tested parameters. The highest values of these parameters were recorded when cyanobacteria were applied as dry + soaking combined with 75 % N rate expect for those of number and nodules the dry weight of nodules that gave their highest values when the lupine plants received the treatment of dry + soaking combined with 50 % N rate. In conclusion, the use of cyanobacteria along with rhizobia as renewable nitrogen source for lupine production can save 25% N from that required for lupine.