Different formulations of pepper mint oil, i.e. bulk, nano-emulsion and nano-encapsulation were evaluated against 2nd and 4th instar larvae of the cotton leafworm Spodoptera littoralis (Boisd.). Their effects on enzymatic activities and bionomics were also investigated. Transmission Electron Microscopy (TEM) showed that the mean particle size of the prepared nano-emulsion ranged between 20-90nm. The encapsulation efficiency and loaded capacity percentages exhibited that distilled H2O was more suitable than ethanol in preparation of nano-encapsulation particles. Bioassay treatments showed LC50's on 2nd instars larvae were 70.59, 12.23 and 21.72 ppm for pepper mint oil, pepper mint nano-emulsion and loaded nanoemulsion, respectively. While on 4th instars these values were 80.47, 26.14 and 67.69 ppm, respectively. LC90's on 2nd instars were 104.75, 33.97 and 30.14 for pepper mint oil, pepper mint nanoemulsion and loaded nano-emulsion, respectively. While on 4th instars these values were 177.66, 71.37 and 102.29 ppm. Toxicity index and relative potency proved that nano-emulsion was more effective than loaded nano-emulsion, while the bulk preparation was the least effective one. Results of enzymatic activities showed marked effects of the three formulations of mint oil, significant inhibitions  were recorded for amylase, invertase, trehalase, protease and alkaline phosphatase, while significant increase in activities of cuticle phenoloxidase and chitinase. Effects of adding the three formulations to artificial diet of 2nd instar showed significant effects of the insect bionomics. Larvae durations, percentage mortalities, were increased as well as larval malformations. Also, pupal duration, percentage pupal mortality and pupal malformation were increased, while pupal weight was decreased. Adults longevity showed insignificant effects, while female fecundity and egg % fertility showed significant responce.

Laboratory and field trials were carried out at Koom Hamada district and Etay El-baroud research station during 2004/ 2005 season to clarify the molluscicidal ac-tivities of the following: indoxacarb, lufenuron, Bacillus thurringiensis, kurestaci (Bt.) and methomyl against glassy clover snails Monacha cartusiana (Müler). Re-sults indicated that all tested pesticides decreased snails population compared with control. Under the field conditions, the efficiency of the tested compounds were 98.0, 93.4, 93 and 71.58% for methomyl, lufenuron, indoxacarb and Bt. after 5, 6, 13, and 28 days of treatment, respectively. Moreover the effect of LC50 and 0.5 LC50 of these compounds were investigated on some biochemical parameters in vivo. The activities of acetylcholinestrase (AChE), alanine aminotransferases (ALT) , as-partate aminotransferases (AST) and protein contents at different time intervals were evaluated. The specific activity of AChE reached 0.009, 0.002, 0.006 and 0.001 mole/ mg protein/ min for indoxacarb, lufenuron, Bt. and methomyl, respectively, after one day of treatment with (LC50 for each compound). In general, Bt. and in-doxacarb caused slight inhibition on AChE enzyme. All treatments decreased ALT and AST enzyme activity in the tested animals. However, Bt. Showed slight effect on the activity of aminotransferases enzymes.