This study was executed during two successive seasons 2014 and 2015 for evaluating the effect of inoculation with Bradyrhizobium sp, Bacillus megaterium, Bacillus circulans, Glomus macrocarpum and Pseudomonas fluorescens on infection percentage, enzymatic activity, growth characteristics, total phenol, peroxidase and polyphenol oxidase activity in presence or absence of Fusarium oxysporum in sterilized or non- sterilized soil under greenhouse condition. The obtained results observed that the tested strains showed suppression for F. oxysporum in vitro. Similar results were obtained under greenhouse experiment where the inoculation of soil with biofertilizers strains in presence of P. fluorescens showed the lowest records of infection percentage as well as the higher values of enzymatic activity, growth characteristics, total phenol, peroxidase and polyphenol oxidase activity compared to full dose of chemical fertilizers treatment. This study provides evidence for diminishing the adverse evolved from the continues application of chemical fertilizers in high doses and replacing it (at least partially) through biofertilizers and biocontrol application.