In this study, the essential oils of rosemary and sage were used to improve cake by two methods either addition or spraying at different concentra-tion on cup cake after 30 min. from baking. The components of essential oils were identified using Gas Chromatography (GC). These oils were eval-uated for inhibitory effect against some pathogenic bacteria especially that may contaminate cake dur-ing handling and storage periods. The effect of these oils at different levels and methods on the sensory characteristics of the produced cake was studied. The results showed that the addition of essential oil of rosemary or sage to cake formula at 1% level increased the acceptability of cakes. While when the rosemary or sage oil sprayed on the cup cake crust after 30 min. of baking at 0.06% or 0.18% level, resulted in a higher over all acceptability scores compared with the other lev-els and cake control. During the storage periods of cakes, using 1.5 % level of rosemary or 0.5% of sage oil added to the formula level resulted in a good acceptability after four and two weeks re-spectively, of storage periods. By using 0.06% concentrations of sage oil sprayed on cup cake crust after 30 min. of baking, the cake had accept-ed during the four weeks of storage periods. Mold growth appeared after 19 and 28 days due to addi-tion of 1% either rosemary or sage oils to the cake formula Meanwhile, the essential oils sprayed at level of 0.12 % of both, the mold appeared after 29 and 33 days for rosemary and sage respective-ly. Also by using the essential oils of rosemary or sage as spray on cake layers, most of bacterial strains and mold-yeast were not detected during the storage periods of cake at room temperature (25 – 30oC). The inhibition of growth of Salmonel-la and E-Coli was noted at 30 μl /disk of rosemary oil and 40 μl /disk for inhibition growth of Bacil-lus Cereus. While the inhibition growth of Salmo-nella, Staph aureus and E-Coli were observed at 40μl /disk of sage oil and at 50 μl/ disk for inhibi-tion growth of Bacillus Cereus.

Abstract Dried rosemary leaves (Rosmarinus officinalis Lamiaceae), and dried clove buds (Syzygium aromaticum) were irradiated with γ-irradiation by Cobalt-60 as an irradiation source at different doses (0, 5, 10, 15, 20, 25 and 30kGy) as an effective method for sterilization, pasteurization and food decontamination, considered as safe method , and stored for 12months at room temperature (22±3 0C). The evaluation of irradiation depended on the chemical studies and antioxidant activity of extracted essential oils. Results showed significant difference for the essential oils yield (content %) at different doses during storage time. Compounds were fractionated and identified by GC/MS for extracted essential oils from each of non – irradiated and irradiated dried rosemary leaves and dried clove buds samples, the main components of essential oil extracted from either non-irradiated or irradiated rosemary leaves were 1,8 cineole, camphor and γ-pinene 26.36, 12.63 and 16.98%, respectively, while, eugenol was the major component in clove essential oil (which extracted from non-irradiated and irradiated samples (81.69%). No differences were noticed in the % of essential oils constituents. Antioxidant activity as % of DPPH scavenging increased with the increasing of essential oils concentrations (250 up to 2500ppm). Furthermore, the effect of adding these extracted oils from non-irradiated and irradiated samples as well as their mixture (1:1) on the threshold odor scores of sunflower oil (Mean of acceptable odor levels) was observed. Rancimat test shown the high oxidative stability in sunflower oil supplemented with 0.3% clove essential oil which extracted from 15kGy irradiated sample was 11.99 hrs, its relative stability was 144.11% comparing with the oxidative stability of sunflower oil supplemented with 0.5% rosemary essential oil extracted from non-irradiated sample (9.76hrs) with 117.3% relative stability. Adding different concentrations of rosemary, clove buds essential oils which extracted from different irradiation doses samples and their mixture (1:1) caused slightly increasing in oxidative stability of sunflower oil.

rosemary and its oil were used to investigate their effects on diabetic and liver malfunctional rats by administrating them with STZ or CCl4 for three weeks. The therapeutic effects of rosemary extracts (alcoholic and aqueous) and its oil against acute hyperglycemia induced by STZ in rats showed a decrease in the levels of fasting and postprandial blood glucose of rats treated with alcoholic extract of rosemary. In addition, results showed a restoration of these levels of the rats treated with aqueous extract or oil to their normal ranges after 3 weeks. There is a significant difference in lipid profile between rats treated with alcoholic or aqueous extracts and the negative control, where, the HDL in group administrated rosemary oil is significantly higher than both positive and negative control.                  The protective effects of rosemary extracts (alcoholic and aqueous) and its oil against liver toxicity induced by CCl4 in rats showed a significant difference between both (negative and positive control) and the activity of AST and ALT in the rats treated by alcoholic and aqueous extracts of rosemary. The protective effects of the plant extracts against liver toxicant occurred by decreasing the activities of these enzymes. Oil rosemary had restored effect more than both ethanolic and aqueous extracts, but not significant. This protective effect occurred by decreasing the activities of AST, ALT and Alp enzymes. No significant (p≤0.05) differences were noticed among the levels of serum total protein for all treatments compared to the negative and positive controls. The levels of total cholesterol and triglycerides recorded high values for the positive controls compared to the negative control. The protective effects of rosemary extracts against liver toxicant occurred by restoring the levels of these parameters to their normal values.