The purpose of the research was to illustrate the effects of feeding Nile tilapia fingerlings (Oreochromis niloticus) with low doses of aflatoxins (AFs) with and without vitamin C supplementation to investigate the capacity of vitamin C in detoxification. Ten experimental diets were formulated and divided into three categories. The first category included: T1, T4 and T7 feedings included 20, 40 and 80 μg AFs kg−1 feed respectively, while both the second category T2, T5 and T8 and the third category T3, T6 and T9 were treated with contaminated feed with AFs for 57days then both categories shifted to different regime till the conclusion of the experimentation. The second category was fed uncontaminated feed while the third category was fed a supplemented diet with 100 mg Kg-1 of vitamin C. For 113 days the experiment was conducted. The results showed that shifting from a contaminated diet to an uncontaminated diet (category 2) or adding vitamin C to the contaminated diet (category 3) improved the deterioration that occurred in the values of growth performance, biochemical parameters and histological disorders caused by AFs. Furthermore, the results from the control group were superior to all the treatments.

Heat stress is an environmental problem that can negatively impact tomato productivity by reducing fruit setting and disrupting pollen development. The present study aims to understand the molecular mechanism of heat tolerance induced by L-ascorbic acid. Plants were grown in the summer of 2022 (day and night temperatures were 34.4°C and 25.2°C respectively). The plants were divided into two groups: one was sprayed with L-ascorbic acid (0.5 mM) and the other served as a control. Growth parameters, antioxidant enzymes, proline, hydrogen peroxide, and malondialdehyde levels in the leaves were determined. Additionally, a molecular docking allowed understanding the interaction between the antioxidant enzymes and L-ascorbic acid. Results indicated that L-ascorbic acid increased antioxidant enzyme activities (catalase, peroxidase, ascorbate peroxidase, polyphenol oxidase), proline content, fruit setting and growth indicators (shoot and root fresh and dry weight). It also reduced hydrogen peroxide and malondialdehyde levels in tomato leaves. Furthermore, the in-silico analysis revealed that L-ascorbic acid binding energies toward antioxidant enzymes were similar to those of known activators. In conclusion, foliar application of L-ascorbic acid (0.5 mM) effectively mitigated heat stress effects, activating antioxidant enzymes and eliminating reactive oxygen species, ultimately resulting in increased fruit setting.

The purpose of the research was to illustrate the effects of feeding Nile tilapia fingerlings (Oreochromis niloticus) with low doses of aflatoxins (AFs) with and without vitamin C supplementation to investigate the capacity of vitamin C in detoxification. Ten experimental diets were formulated and divided into three categories. The first category included: T1, T4 and T7 feedings included 20, 40 and 80 μg AFs kg−1 feed respectively, while both the second category T2, T5 and T8 and the third category T3, T6 and T9 were treated with contaminated feed with AFs for 57days then both categories shifted to different regime till the conclusion of the experimentation. The second category was fed uncontaminated feed while the third category was fed a supplemented diet with 100 mg Kg-1 of vitamin C. For 113 days the experiment was conducted. The results showed that shifting from a contaminated diet to an uncontaminated diet (category 2) or adding vitamin C to the contaminated diet (category 3) improved the deterioration that occurred in the values of growth performance, biochemical parameters and histological disorders caused by AFs. Furthermore, the results from the control group were superior to all the treatments.