The present work is concerned with optimization the process parameter of the production of fungal α-amylase enzyme by Aspergillus niger ATCC 102 and Aspergillus oryzae NRRL 6270 using solid-state fermentation (SSF) process on two agro-industrial by-products. Different cultural parameter and conditions such as moisture content of prepared agro-industrial by-product (30 to 70%), fermentation period (12 to 120 h.) and incubation temperature (25-35°C) were optimized to obtain the maximum yield of α-amylase activity. The study is concerned to produce fungal αamylase on agro-industrial by-products such as inner layers of sugarcane bagasse (Saccharum officinarum) (SCB) and corn gluten meal (Zea mays) after addition of germ cake and corn steep liquor (CGM ) were used as solid substrates. The optimum conditions for SSF as follows temperature at 30°C and two moisture content level (60 - 30%) for SCB by Aspergillus niger and CGM after addition of germ cake and corn steep liquor by two fungal strains, respectively and incubation period of 72, 48, 24 hours for SCB by Aspergills niger, CGM by two fungal strains, respectively and SCB by Aspergillus oryzae, producing α-amylase activity 3.49U/g (solid substrate) with SCB by SSF after 24 hours by Aspergillus niger, whereas 3.78U/g (solid substrate) by Aspergillus oryzae after 48 hours. The highest record of amylase activity was obtained on SCB by SSF 3.49U/g (solid substrate) after 24 hours by Aspergillus niger, whereas 3.78U/g (solid substrate) by Aspergillus oryzae  after 48 hours. Optimum α-amylase enzyme activity was observed at 30°C. Utilization of agroindustrial by-product provides an alternative method and value-addition in cost effectiveness of bioprocess. The obtained results demonstrated that, the potential application of the used strategy for αamylase enzyme production from agro-industrial by-products.

This study was executed during two successive seasons 2014 and 2015 for evaluating the effect of inoculation with Bradyrhizobium sp, Bacillus megaterium, Bacillus circulans, Glomus macrocarpum and Pseudomonas fluorescens on infection percentage, enzymatic activity, growth characteristics, total phenol, peroxidase and polyphenol oxidase activity in presence or absence of Fusarium oxysporum in sterilized or non- sterilized soil under greenhouse condition. The obtained results observed that the tested strains showed suppression for F. oxysporum in vitro. Similar results were obtained under greenhouse experiment where the inoculation of soil with biofertilizers strains in presence of P. fluorescens showed the lowest records of infection percentage as well as the higher values of enzymatic activity, growth characteristics, total phenol, peroxidase and polyphenol oxidase activity compared to full dose of chemical fertilizers treatment. This study provides evidence for diminishing the adverse evolved from the continues application of chemical fertilizers in high doses and replacing it (at least partially) through biofertilizers and biocontrol application.

The aim of this study was to evaluate effect of different level of probiotic supplementation to ruminant rations, using in-vitro batch culture technique to determine degradation and fermentation parameters. In vitro experimental ration was formulated, the ration consisted of 40% alfalfa hay and 60% concentrate feed mixture. Three level of probiotic supplementation (106, 108, 1010 cfu/kg DM) were evaluated. DM and total gas production as well as fermentation parameters of the incubated samples were determined after 24 hrs. of fermentation. Slightly increases (P>0.05) in in-vitro dry matter degradability were observed for the ration supplemented with probiotics bacteria at different levels (106,108 and 1010 cfu/ kg DM) compared to control ration. Probiotics bacteria supplementation with different level (106,108 and 1010 cfu/ kg DM) led to significant (P<0.001) increases in organic matter degradability and total gas production per sample and per g DM, OM, NDF and ADF compared to the not supplemented ration (control ration), and no significant differences were observed among the different levels of probiotics supplementation. Significant increase in total volatile fatty acid concentration after 24 hours' incubation period compared to the not supplemented ration. On the other hand, the treatment supplemented with probiotic recorded lower ammonia concentration compared to the control group. It could be concluded that, adding  probiotics bacteria supplementation to experimental ration resulted increase DM and OM degradability and using dose 106 CFU/kg DM feed is sufficient to induce improvement in degradability and fermentation parameters

Different types of drinking yoghurts were made with different types and concentrations of stabilizers. 6 treatments were made (T1, T2, T3, T4, T5 and T6) using 0.2% and 0.4% of different types stabilizers ( pectin, guar gum, and mixture (1:1) of both pectin and guar gum) for production of other yoghurt drink treatments beside the control without any stabilizers. All treatments were stored up to 14 days at 5±1°C. Drinking yoghurt samples were evaluated for chemical, reholigical, microbiological and sensory attributes. No significant differences were observed in chemical and microbiological properties among control and treated samples. All drinking yoghurt contained levels of (106–107 cfu/g) lactic acid bacteria at the end of the refrigerated storage. Addition of stablizers showed significant differences in viscocitey and serum separation of final product. The effect was more obvious with using 0.2% stabilizer mixture (0.1% guar gum + 0.1% High Methoxy Pectin) than other all treatments. The drinking yoghurt containing 0.2% mix stabilizers (0.1% guar gum + 0.1% High Methoxy Pectin) ranked higher sensory scores than other treatments. The best drinking yoghurt that containing 50% yoghurt, 8% sugar and 42% water should be fortified with 0.2% mix stabilizers (0.1% guar gum + 0.1% High Methoxy Pectin)

The aim of this work is developing and evaluating an automated measurement and system datalogger for continuous monitoring of solar hot air drier for natural convection solar cabinet drier. Measurements includes monitoring of drying process for sliced Banana (Musa acuminata) relation to weather parameters such as ambient air temperature, relative humidity and wind velocity which ensures precise measurements and reproduction of experiments. An electronic measurement system was used for measurement of temperature, humidity, air speed, a solar intensity. A programmable microcontroller ship and code were used to control measurement, timing, and data storage twenty-four hours/day. All measurements data was collected and saved in storage memory card. Results showed that the solar drier drying rate achieved (-0.018gH2O/g dm.h), while the drying rate with mean of electrical thermal drier were  (-0.956 018gH2O/gdm.h) at 105°C, (-0.552 018gH2O/g dm.h) at 70°C and (-0.513 018gH2O/gdm.h) at 50°C. The electronic measurement - datalogging system was able to give an accurate measurement and assessment to the drying system and process. It was found that air leakage from the system and poor insulation inhibit the airflow over the solar absorber plate and drying chamber causing thermal leakage form the bottom and drier sides. It was recommended to supply the solar drier with an air fan, seal the drying air leakage, and air-lock at the entrance to control airflow as well as the temperature of the drying process.

Due to the climatic changes in Egypt, the remarkable population growth and the high demand for water, especially for irrigation water. To rationalize the use of irrigation water, it has been  applied efficient irrigation systems such as trickle irrigation system for irrigating trees and plants by securing the least amount of water for the plant without wasting and saturation of the surrounding area. This study aims to evaluate the performance of some local and imported emitters. In this study, 16 emitters were used (9 imported emitters and 7 local emitters) divided into (9 non-pressure compensating and 7 pressure compensating), for evaluation under different operating pressures (0.5, 0.75, 1, 1.25 and 1.5 bar), in addition to measure the hydraulic performance of the manufacture's coefficient of variation (CV %), emitter flow variation (     %), and the emission uniformity (EU %). The results showed that the highest emission uniformity value for emitters of (OT1, OT2, KF, TKY, CKF and OT3), with discharge of (2) L / h non- pressure compensating (NPC), 4 l/h non-pressure compensating (NPC), 4 l / h pressure compensating (PC), 8 l/ h non- pressure compensating (NPC),  8 l / h pressure compensating (PC) and 16 l / h non-pressure compensating (NPC) respectively, while, the lowest manufacture's coefficient of variation value (CV%), emitter flow variation (     %) were used with 6  emitters in the second part of the laboratory experiment to evaluate the side lines under the length of the hoses (50, 75, 100 m) at a distance between the emitters (4 m), for the calculation of the emission uniformity (EU%), friction  losses and consumption of power. From the last result in laboratory and through evaluation the 6 emitters under study in terms of prices and emission uniformity (EU%), a randomized field study was conducted on the farms where the three emitters (OT2, KF and OT1) under lateral length (50 m) and emitter spacing (4 m). The purpose of the field test was carried out to determine the degree of clogging throughout the operating period (after 2 and 4 years). A field test used an emitter (OT1, OT2 and KF) found for two months and has been used again for two years when farming in the farm was expanded and used four years ago by expanding the farm, the emitter (OT2) clogging ratio was 3.09% within two months, 6.95% within two years and 10.49% within 4 years, the emitter (OT1) clogging ratio was 5.26% within two months, 11.11% within two years and 17.64% within 4 years, the emitter (KF) clogging ratio was 6.83% within two months, 13.63% within two years and 20.96% within 4 years. The results showed that the lowest degree of clogging ratio of the emitter (OT2) was within two months of operation. In general, and as expected, the results indicate that clogging ratio increased with increasing the time of installation of the field emitter and the range of factors affecting the periodic maintenance and design of good and components of irrigation network with high quality and ratio of manufacture's coefficient of variation (CV%).

The effect of different concentrations of ascorbate (As) at 0, 0.25, 0.5 and 1.0 mM, salicylate (Sa) at 0, 0.25, 0.5 and 1.0 mM , in addition to silicate (Si) at 0, 100, 250, and 500 ppm on potato plant subjected to different water regimes; three days interval (control), four days interval (4DI) and five days interval (5DI).  Photosynthetic pigments, free proline and malonyldialdehyde (MDA) concentrations, and plant dry matter percentage were determined. Also, tuber yield was recorded and the percentage of tuber dry matter was determined. Water deficit resulted in significant reduction in tuber yield, plant dry matter percentage beside significant increase in free proline and MDA contents. Meanwhile, effects on photosynthetic pigments content and tuber dry matter were inconsistent. The effects of using different concentrations of ascorbate (As) under water deficit provide a superior effect of As at 0.25 mM. Data revealed that ascorbate treatment enhanced tuber yield by improved Chl (a and b) and carotenoids content, with a promoted reduction in free proline content and MDA. Salicylate (Sa) treatment under water deficit exhibited partial enhancement in photosynthetic pigments; where, Sa (1.0 mM) increased Chl a under all water regimes and Chl b at control and 4DI water regimes. While Sa (0.5 mM) improved carotenoids content at 5DI water regimes. The positive effect of salicylate on tuber yield was obvious only at 5DI water regime; as salicylate concentrations (0.25, 0.5 and 1.0 mM) enhanced tuber yield by 65.61, 21.8 and 33.33%. Data exhibited that these increments were concomitant with a  reduction in free proline content and a slight increase in tuber dry matter. Interactive effects of Silicate (Si)/water deficit were variable with the concentration under the used water deficit regimes, where, Si (100 ppm), showed higher means of Chl (a and b) with a reduction in free proline contents, at both 4DI and 5DI. The higher tuber yield resulted only at 5DI. More pronounced effect, Si (250 ppm) exhibited increment of photosynthetic pigments, plant dry matter, tuber yield, along with slight reduction in MDA, particularly at 5DI. It could be concluded that silicate treatment alleviated water stress by improving carotenoids content, plant dry matter and reducing both free proline and MDA contents, which attributes enhancement in tuber yield under water deficit. Generally, lower concentrations of As, Sa and Si showed their benefits under higher water stress regimes.

The main objective of this study was to evaluate different samples of  corn silage (Whole corn plant with ears) from different regions in Egypt to determine each sample has the highest nutritive value and nutrients digestibility and therefore reduce the feed cost, increase feed efficiency and probability of lactating dairy farms. Silage samples were collected from different four areas in Egypt; El-Salhya, El-Nobarya, El-Monofia and Ganakles. The study Included three field and laboratory work cores which were; chemical composition, in-vitro and in-situ evaluation studies. The samples were analyzed for its chemical composition, Dry matter (DM), crude protein (CP), neutral detergent fiber (NDF), acid detergent fiber (ADF) and acid detergent lignin (ADL). The silage samples were evaluated in-vitro and in-situ. In-vitro evaluation was conducted using gas production technic. Gas production (GP) was recorded at 0, 2, 4, 8, 16, 24 and 48 hours of incubation. The organic matter digestibility (OMD), short chain fatty acids (SCFA), and metabolizable energy (ME) were measured. The in-situ experiment, involved nylon bags containing silage samples from different regions were incubated in three fistulated Barki rams for 24h. Samples were taken out at 2, 4, 8, 12 and 24h of incubation. The obtained results indicated that the Ganakles silage ampel recorded the lowest (P<0.01) values of GP within the different times compared to others. The silage from El-Salhya had the highest (P<0.01) values for OMD and SCFA. The DMD of in-situ samples was significantly (P<0.01) increased within the different times of incubation with Al-Salhia silage samples, but the lowest significant values (P<0.01) were recorded  with Al-Nobaria silage samples. Depending on invitro and in-situ results for silage type results, in vitro and in-situ for silage type from Al-Salhia area had a best characteristics of good silage and was chosen forever conducting lactation trial.

Two pot experiments were conducted on the 17th and 11th of march during 2015 and 2016 growth seasons respectively at the greenhouse, Dept. Agric. Botany, Faculty of Agriculture, Ain Shams University, Shoubra El-Kheima, Egypt to investigate the impact of drought stress on some growth, biochemical and anatomical parameters of Thymus vulgaris L. Plants were exposed to two different irrigation levels: 70-80% and 30-40% of water holding capacity (WHC) as well-irrigated and drought stressed plants respectively. The results indicated that all investigated growth parameters and leaf photosynthetic pigments were decreased significantly by exposing to drought stress. There were significant increase in the concentration of reactive oxygen species (ROS) as indicated by measuring of H2O2 and malondialdehyde (MDA). A similar trend was observed in respect to osmolytes including proline, total soluble sugars and free amino acids. Also, drought stress increased significantly total soluble phenols and the specific activity of Phenylalanine ammonia-lyase (PAL) and Polyphenol oxidase (PPO), whereas, no significant differences were detected in peroxidase (POD). The anatomical examination showed that there were several significant changes associated with water limited supply in the stem and leaf of plants.

The objective of this study was to study the effect of laser irradiation on maturation rate of dromedary she-camel oocytes.  Although in vitro fertilization (IVF) technique in she-camel has been established, but maturation rate of camel oocytes is still low comparing with other animal species. Several studies performed to improve in-vitro maturation rate using different types of media with different incubation times. In order to establish high sensitive and low cost maturation improvement technique, laser irradiation has been suggested in the present work. Cumulus oocytes complexes (COCʼs) were collected from ovaries by aspiration method and grade (A) oocytes were chosen and divided into five different groups, 62 oocytes served as control group,  an un-irradiated (group 1), 64 oocytes exposed to 2 minutes of laser irradiation (group 2), 57 oocytes exposed to 3 minutes of laser irradiation (group 3), 49 oocytes exposed to 4 minutes of laser irradiation (group 4) and 52 oocytes exposed to 5 minutes of laser irradiation (group 5) with a total output power of 3 mW for different exposure durations; 2, 3, 4 and 5 minutes.  Afterwards, oocytes were matured in TCM-199 medium at 38.5oC and 5% CO2 in humidified air for 42 h. Maturation rate was calculated based on expulsion of the first polar body and statistically analyzed by one way ANOVA test.   The obtained results showed that, the oocytes reached germinal vesicles (GV) which exposed to laser beam for 5 minutes at 488 nm wavelength represent significantly (P<0.05) the highest value (42.31%) compared to control (not irradiated, 16.13%). However, other groups of GV showed insignificant differences with the control group. The metaphase II (M II) in the control oocytes represents significantly (P<0.05) the highest value (75.81%) compared to 3-5 minutes exposed groups. The degenerated oocytes exposed to laser beam for 5 minutes at 488 nm wavelength represent significantly (P<0.05) the highest value (40.38%) compared to control (not irradiated, 8.06%). In conclusion‚ these results indicated that the exposure of laser irradiation for 2 minutes may improve in-vitro nuclear maturation of immature oocytes in dromedary she-camels as compared to other durations (3-5 minutes) at 488 nm wavelength (blue laser).