The population genetic and molecular evidence of the Arabian goat breed's phylogenetic standing (Capra hircus) is misunderstood, and little is known about genetic diversity in this species. Despite their importance in producing meat and milk, as well as their capacity to endure difficult conditions with little access to food and water, goats are the most common animal in Arab countries. We investigated the phylogenetic history and demographics of domestic goats by sequencing a hypervariable segment of the mtDNA reference region for Baladi, Demuscous, and Zaraibi breeds, as well as sequences retrieved from 16 Arab countries. Three significantly divergent lineages associated with Africa and the Middle East were identified through phylogenetic analysis of the investigated strains of Egyptian goats' mtDNA D-loop region. All Egyptian goats were allocated to lineage A, which is made up of 445 different haplotypes, including Egyptian and Arabian goats. Diversity in haplotypes and in nucleotides for each Arabian goat population was calculated separately and estimated to be 0.99835 and 0.02576, respectively. Finally, we suggest that all goat lineages descend from a single clan. The ancient world's migration and travel may have influenced goat diversity, implying that goats had multiple matrilineal forebears

The experiment was carried out on three different vegetable crops, Cucumber Cucumis sativus L. (Cucurbitaceae), eggplant Solanum melongena L. (Solanaceae) and okra Abelmoschus esculentus L. (Malvaceae), during summer 2021, to investigate the effect of vegetation type on spider density and diversity. For this study, the pitfall trap method was used to collect spiders from the soil surface to ensure a comprehensive representation of all spider guilds. During this study, 20 species belonging to 6 families were collected. The results showed that a total of 374, 285 and 194 individuals belonging to 18, 17 and 15 species were recorded in okra, cucumber and eggplant fields respectively. Species diversity was greater in okra cultivation. Results also indicated that the Lycosidae family was the most abundant and dominant, representing 71.75%, followed by the Linyphiidae family of 90 individuals representing 10.55% of the whole obtained population. The most dominant species were Wadicosa fidelis 152, 98 and 67 individuals and Pardosa injucunda 111, 69 and 52 individuals in okra, cucumber and eggplant respectively. It is inferred from this study that the type of plant can have an impact on spider communities.

Nine Penicillium digitatum isolates were isolated from citrus fruits and tested for their pathogenicity on Navel orange fruit (Citrus sinensis L.). In this regard, the isolates exhibited three different levels of virulence. The isolates were identified morphologically using malt extract agar (MEA) and Czapek yeast extract agar (CYA) for colony characteristics, and MEA for microscopic features. Each one of the isolates has the unique features of P. digitatum, especially the largest conidia (6.1-11.9 µm long X 3.2-8 µm width), and phialides (10.1-21.4 µm long X 4-5.1 µm width). In addition, the isolates were identified on a molecular basis using ITS1 and ITS4 primers to confirm morphological identification. Phylogenetic analysis revealed that there was high variance among the isolates, and there were different relations between some of our isolates and other P. digitatum registered strains originating from different countries all over the world. The sequences were submitted to respective GenBank nucleotide databases with accession No. OR198852, OR198853, OR198854, OR198855, OR198856, OR198857, OR198858, OR198859 and OR198860. We also isolated a new P. digitatum (OB15: OR198859) strain with high virulence and rapid sporulation

Worldwide, potato (Solanum tuberosum L.) is one of the most essential agro-economically food crops. To ensure its presence throughout the year for food processors and to extend its shelf life, it should be stored in cold temperatures. Despite the benefits of storage at low temperatures, it causes undesirable phenomena; one of them is cold-induced sweetening, which reduces the quality and the commercial value of the potato tubers. In this study, the CRISPR technology ″Clustered Regularly Interspaced Short Palindromic Repeats″ has been used in potato cultivar Desiree via Agrobacterium mediated transformation to edit starch phosphorylase gene that is responsible for starch degradation in cold temperature storage, after regeneration steps. The regenerated plants were used in the screening of genome editing by Sanger sequencing and the ICE program. Screening results showed that Desiree 2 (DE2), Desiree 7 (DE7) and Desiree 27 (DE27) lines have mutations in sgRNA sequence that indicated a significant reduction in reduced sugar contents of tubers after cold storage for 90 and 120 days. The reduction mean values were 29.224, 35.078 and 35.862 in DE2, DE7 and DE27 respectively after 90 days and 83.212, 56.674 and 34.109 after 120 days without visible differences in plant morphology and tubers

Heat exchanger optimization is a very efficient thermal design inquiry. The goal of the research is to determine an optimal channel flow velocity as well as an optimal total plate number based on overall information on the effectiveness of employing chilled water to cool soybean milk. Heat transfer coefficient, Prandtl number, Reynolds number and pressure drop measurements were applied for milk and water. The rheological properties of soybean milk have been investigated at various temperatures (5, 10, 30, 50, 70 and 90 °C). The operational circumstances of PHE in soybean procedures were investigated at different numbers of plates, flow rates and flow velocities. Based on the present study, total substances showed non-Newtonian pseudoplastic conduct in any respect at any temperature and the power law model has a decent correlation. The cost estimation of the plate heat exchanger was observed at the lowest flow velocity of (2.25) m/s and number of plates is 17.

Congo red (CR) is an anionic dye that is released from various industries and demonstrates a negative effect on the environment and human health. Dye`s elimination from the environment is an important challenge; therefore, the aim of this study was the application of unhydrolyzed sugarcane bagasse wastes, after acid hydrolysis, drying, grinding and including cellulose acetate to form different composite sheets. The composite sheets were applied in the 3Rs (Removal of Congo red using the composite sheet, Recover of the Congo red after removal using ethanol, and Reuse of the composite sheet many times) processes. The Congo red removal efficiency by the composite sheets was optimized using two statistical methods, One Factor at A Time (OFAT) and General Factorial Design. Afterward, isotherms models and kinetics studies were investigated; moreover, the reusability was also examined. The results exhibited that, the Congo red removal efficiency of 76.27 % was achieved for 500 ppm of the dye using the composite sheet with a concentration of 7 g/L; at a pH value of 7.0 and a contact time of 240 min. Interestingly, the composite sheets have been applied for 3Rs (Removal, Recovery, Reuse) cycles 5 times.

Ephedra is the most widely and largest widespread genus in the Gnetopsida, a subgroup of the gymnosperms. This research was done to find out how gamma radiation affected the in vitro growth of callus and suspension cultures. In addition, the effect of gamma rays (γ-rays) on the ephedrine and pseudoephedrine concentrations was evaluated through HPLC analysis. The cell suspension and callus cultures were formed on Murashige and Skoog's basal medium (MS) supplemented with 0.5 mg/l kinetin (Kn), and 3 mg/l of 2, 4-dichlorophenoxy acetic acid (2,4-D) and exposed to variable gamma radiation doses (0, 5, 10, 15, 20, 25 and 30 Gray). Highly significant differences in both fresh and dry callus weights were recorded due to the effect of gamma rays (γ-rays). The findings demonstrated that in the cell suspension and callus cultures, 15 Gray achieved the highest fresh and dry weights when compared to the control. Similarly, the highest concentrations of ephedrine and pseudoephedrine were found in suspension and callus cultures compared to control at 15 Gray.

Sugar beet (Beta vulgaris L.) is the second sugar crop for sugar production after sugar cane worldwide. This plant is subjected to the infestation of several insect pests that cause considerable damage, especially leafhoppers, that can transmit phytopathogens (e.g., phytoplasma). In this work, we identified and described the leafhopper, Hebata (Alboneurasca) decipiens, in addition to the detection and molecular identification of Candidatus Phytoplasma that was transmitted by this leafhopper on sugar beet plants from naturally infected sugar beet plants to an uninfected one. The 16S rDNA gene from phytoplasma was amplified by a nested polymerase chain reaction (PCR) assay and directly sequenced using specific primer pairs DNA fragments. The resulting sequences were analyzed and compared with another phytoplasma sequence available at GenBank sequences performing BLAST using DNAMAN.

Nile tilapia is a freshwater fish of African origin, with productive and economic characteristics affecting global fish markets. The Oreochromis niloticus reference genome has a diploid set of 22 linkage groups (LGs) in addition to ungrouped sequences. A total of 42,622 genes have been identified, but 550 genomic gaps may include more. Our study focused on some of these genomic gaps, where appropriate primers were designed and then multiplied through the polymerase chain reaction (PCR) technique. From local samples, DNA was extracted and amplified with the new primers. Successful amplicons were sequenced and annotated using available bioinformatics tools. Five assessed sequences were annotated, of which three were newly predicted in O. niloticus, as mucin 1-like, and KLR genes, and SATB region. The other two sequences belonged to grid2 and trpm7 which were previously predicted. Although individual attempts to identify genomic gaps are not feasible in filling the large lack of information on the Nile tilapia genome, a good number and valuable new information has been reached. However, the following plan showed many technical problems, more time, effort and cost that could be avoided by suggesting the application of another technique, such as whole-genome sequencing, mapping, and assembly.

This study aimed to evaluate the influence of choline chloride supplementation on the growth performance of feedlot calves. Thirty-two crossbred calves with a mean initial body weight of 305.71±4.09 Kg were divided into three groups of 10, 11 and 11 animals, randomly. The animals were distributed to one of three dietary treatments, control, choline15 and choline 30 (the rations supplemented with 0, 15 and 30 g choline chloride, respectively). The animals were fed on concentrate feed at a rate of about 2.15% of their live body weight and rice straw open access. Supplementing animal ration with choline chloride did not significantly affect all nutrients' digestibility and feeding value. The animals supplemented with 30 g choline chloride recorded significantly higher (P=0.037) total serum protein concentration compared to the other groups. The group received a ration supplemented with 30 g of choline chloride and recorded significantly higher final weight, total gain, and mean daily gain. Data showed significant improvement in TDN, CP and DCP conversion for the animal received ration supplemented with 30 g choline chloride compared to the control group. In conclusion, supplementing fattening calves' ration with rumen-protected choline could improve average daily gain and feed conversion.