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Effect of Foliar Spraying with Pink Pigmented Facultative Methylotrophic Bacteria on the Growth and Productivity of Strawberry Полный текст
2023
shadia Ismail | Fafy Mohammed
Effect of Foliar Spraying with Pink Pigmented Facultative Methylotrophic Bacteria on the Growth and Productivity of Strawberry Полный текст
2023
shadia Ismail | Fafy Mohammed
Pink-pigmented facultative methylotrophic bacteria (PPFM) isolated from cotton leaves was identified based on phenotypic and genotypic characteristics as Methylobacterium rodiotolerance. Two field experiments were conducted to investigate the effects of PPFM, methanol (10 and 30%) and a combination of bacteria and methanol on the growth, fruit quality, and yield of two strawberry cultivars (Florida and Festival). The main differences between the two cultivars are greater foliage fresh weight and early yield in cv. Florida while Festival cv. had a higher total yield per plant and greater anthocyanins and ascorbic acid contents. The greatest vegetative growth, dry matter percentage, potassium content, and carbohydrate content besides the earliest yield per plant were observed following spraying with PPFM or PPFM mixed with 10% methanol. Spraying with PPFM resulted in the highest total yield per plant, highest yield per feddan, and fruit quality. Spraying cv. Florida with PPFM resulted in the best early yield while spraying cv. Festival with PPFM resulted in the highest total yield and fruit quality. Spraying with PPFM appears to be the most efficient treatment for enhancing the total yield of Festival cv. by an average of 23.02 and 24.06 tons per feddan in the first and second seasons, respectively.
Показать больше [+] Меньше [-]Effect of Foliar Spraying with Pink Pigmented Facultative Methylotrophic Bacteria on the Growth and Productivity of Strawberry Полный текст
2023
Ismail, shadia | Mohammed, Fafy
Pink-pigmented facultative methylotrophic bacteria (PPFM) isolated from cotton leaves was identified based on phenotypic and genotypic characteristics as Methylobacterium rodiotolerance. Two field experiments were conducted to investigate the effects of PPFM, methanol (10 and 30%) and a combination of bacteria and methanol on the growth, fruit quality, and yield of two strawberry cultivars (Florida and Festival). The main differences between the two cultivars are greater foliage fresh weight and early yield in cv. Florida while Festival cv. had a higher total yield per plant and greater anthocyanins and ascorbic acid contents. The greatest vegetative growth, dry matter percentage, potassium content, and carbohydrate content besides the earliest yield per plant were observed following spraying with PPFM or PPFM mixed with 10% methanol. Spraying with PPFM resulted in the highest total yield per plant, highest yield per feddan, and fruit quality. Spraying cv. Florida with PPFM resulted in the best early yield while spraying cv. Festival with PPFM resulted in the highest total yield and fruit quality. Spraying with PPFM appears to be the most efficient treatment for enhancing the total yield of Festival cv. by an average of 23.02 and 24.06 tons per feddan in the first and second seasons, respectively.
Показать больше [+] Меньше [-]Molecular Identification of Some Rhizobium and Serratia Isolates as Potential Producers of Indole-3-Acetic Acid Полный текст
2023
Shaimaa Sello | Ashraf Abdel Razik | Samir Ibrahim | Sawsan Elateek
Molecular Identification of Some Rhizobium and Serratia Isolates as Potential Producers of Indole-3-Acetic Acid Полный текст
2023
Shaimaa Sello | Ashraf Abdel Razik | Samir Ibrahim | Sawsan Elateek
In order to reduce the use of chemical fertilizers, the use of biological inoculants has been increased. Beneficial microorganisms are used to increase crop yields by stimulating plant growth through the production of phytohormones. In the present study, Indole Acetic Acid (IAA) production was analyzed in 18 bacterial isolates with Plant Growth-Promoting (PGP) capabilities; 15 of these isolates were Rhizobium, and three were Serratia. All isolates have been characterized morphologically and biochemically, and their IAA production in the presence of tryptophan, a precursor for IAA biosynthesis, has been evaluated. IAA production was detected using the Salkowski reagent with CT-2200 spectrophotometer at 530 nm. The levels of IAA production varied between the different isolates. The top two IAA producers were selected for genetic identification using 16SrRNA primers (27F and 1492R). One of the Rhizobium isolates (NRC-R2) shared 95% sequence similarity with Rhizobium sp., according to a Blastn search in GenBank and Rhizobium leguminosarum, whereas the Serratia isolate (Ain Shams Center for Genetic Engineering and Biotechnology (ACGEB)-S2 was 99.5% similar to Serratia sp. Accordingly, these two isolates could serve as biological sources for IAA.
Показать больше [+] Меньше [-]Molecular Identification of Some Rhizobium and Serratia Isolates as Potential Producers of Indole-3-Acetic Acid Полный текст
2023
Sello, Shaimaa | Abdel Razik, Ashraf | Ibrahim, Samir | Elateek, Sawsan
In order to reduce the use of chemical fertilizers, the use of biological inoculants has been increased. Beneficial microorganisms are used to increase crop yields by stimulating plant growth through the production of phytohormones. In the present study, Indole Acetic Acid (IAA) production was analyzed in 18 bacterial isolates with Plant Growth-Promoting (PGP) capabilities; 15 of these isolates were Rhizobium, and three were Serratia. All isolates have been characterized morphologically and biochemically, and their IAA production in the presence of tryptophan, a precursor for IAA biosynthesis, has been evaluated. IAA production was detected using the Salkowski reagent with CT-2200 spectrophotometer at 530 nm. The levels of IAA production varied between the different isolates. The top two IAA producers were selected for genetic identification using 16SrRNA primers (27F and 1492R). One of the Rhizobium isolates (NRC-R2) shared 95% sequence similarity with Rhizobium sp., according to a Blastn search in GenBank and Rhizobium leguminosarum, whereas the Serratia isolate (Ain Shams Center for Genetic Engineering and Biotechnology (ACGEB)-S2 was 99.5% similar to Serratia sp. In the near future, it is possible that these two isolates could serve as biological sources for IAA.
Показать больше [+] Меньше [-]Evaluation of Certain Synthetic and Natural Pesticides Against Whitefly Bemisia tabaci (Genn.) on Green Bean and Effect on Honeybee Apis mellifera L. Полный текст
2023
Sharihan Ahmad | Walaa Abdelghany | Ahmed Ahmed | Sawsan Abdelmegeed
Evaluation of Certain Synthetic and Natural Pesticides Against Whitefly Bemisia tabaci (Genn.) on Green Bean and Effect on Honeybee Apis mellifera L. Полный текст
2023
Sharihan Ahmad | Walaa Abdelghany | Ahmed Ahmed | Sawsan Abdelmegeed
The green bean crop (Phaseolus vulgaris) is infested with many insects such as whiteflies (Bemisia tabaci). An investigation was carried out to study the insecticidal activity of whitefly (Bemisia tabaci), five pesticides were used, namely Thiamothoxam, Sulfoxaflor, Spirotetramat, Pymetrozine, and Flonicamid, and three commercial natural pesticides, namely Superrego, Topnine, and Bernastar. Pesticides and natural pesticides were sprayed during the two seasons of 2020 and 2021. The obtained results in terms of the reduction rate of the insects' number were as follows: The highest rate of reduction appeared in Thiamothoxam and Sulfoxaflor treatments, where it reached above 80%, while the other pesticides were in the range of 40% to 60%, and the lowest reduction rate was 34.33% and 36.00 % for Bernastar during season 2020 and 2021 respectively. When evaluating the toxicity of these pesticides and natural pesticides against honeybees (Apis mellifera), the pesticides did not reveal any toxicity to honeybees, except for Thiamothoxam, Sulfoxaflor, and Superrego The LC50 and LD50 values against honeybees were 0.2 ppm and 0.000005µg/one honeybee for Thiamothoxam, 4.5 ppm and 0.0001125 µg/one bee for Sulfoxaflor, and 0.95 ppm and 0.00002375 µg /one bee for Superego respectively.
Показать больше [+] Меньше [-]Evaluation of Certain Synthetic and Natural Pesticides Against Whitefly Bemisia tabaci (Genn.) on Green Bean and Effect on Honeybee Apis mellifera L. Полный текст
2023
Ahmad, Sharihan | Abdelghany, Walaa | Ahmed, Ahmed | Abdelmegeed, Sawsan
The green bean crop (Phaseolus vulgaris) is infested with many insects such as whiteflies (Bemisia tabaci). An investigation was carried out to study the insecticidal activity of whitefly (Bemisia tabaci), five pesticides were used, namely Thiamothoxam, Sulfoxaflor, Spirotetramat, Pymetrozine, and Flonicamid, and three commercial natural pesticides, namely Superrego, Topnine, and Bernastar. Pesticides and natural pesticides were sprayed during the two seasons of 2020 and 2021. The obtained results in terms of the reduction rate of the insects' number were as follows: The highest rate of reduction appeared in Thiamothoxam and Sulfoxaflor treatments, where it reached above 80%, while the other pesticides were in the range of 40% to 60%, and the lowest reduction rate was 34.33% and 36.00 % for Bernastar during season 2020 and 2021 respectively. When evaluating the toxicity of these pesticides and natural pesticides against honeybees (Apis mellifera), the pesticides did not reveal any toxicity to honeybees, except for Thiamothoxam, Sulfoxaflor, and Superrego The LC50 and LD50 values against honeybees were 0.2 ppm and 0.000005µg/one honeybee for Thiamothoxam, 4.5 ppm and 0.0001125 µg/one bee for Sulfoxaflor, and 0.95 ppm and 0.00002375 µg /one bee for Superego respectively.
Показать больше [+] Меньше [-]Morphological and Molecular Identification of Penicillium digitatum Causing Green Mould of Citrus Fruits in Egypt Полный текст
2023
Abdalrahman Desouki | Labiba Ahmed Reda | Mohamed Rashed | Shehata Shehata
Morphological and Molecular Identification of Penicillium digitatum Causing Green Mould of Citrus Fruits in Egypt Полный текст
2023
Abdalrahman Desouki | Labiba Ahmed Reda | Mohamed Rashed | Shehata Shehata
Nine Penicillium digitatum isolates were isolated from citrus fruits and tested for their pathogenicity on Navel orange fruit (Citrus sinensis L.). In this regard, the isolates exhibited three different levels of virulence. The isolates were identified morphologically using malt extract agar (MEA) and Czapek yeast extract agar (CYA) for colony characteristics, and MEA for microscopic features. Each one of the isolates has the unique features of P. digitatum, especially the largest conidia (6.1-11.9 µm long X 3.2-8 µm width), and phialides (10.1-21.4 µm long X 4-5.1 µm width). In addition, the isolates were identified on a molecular basis using ITS1 and ITS4 primers to confirm morphological identification. Phylogenetic analysis revealed that there was high variance among the isolates, and there were different relations between some of our isolates and other P. digitatum registered strains originating from different countries all over the world. The sequences were submitted to respective GenBank nucleotide databases with accession No. OR198852, OR198853, OR198854, OR198855, OR198856, OR198857, OR198858, OR198859 and OR198860. We also isolated a new P. digitatum (OB15: OR198859) strain with high virulence and rapid sporulation
Показать больше [+] Меньше [-]Morphological and Molecular Identification of Penicillium digitatum Causing Green Mould of Citrus Fruits in Egypt Полный текст
2023
Desouki, Abdalrahman | Ahmed Reda, Labiba | Rashed, Mohamed | Shehata, Shehata
Nine Penicillium digitatum isolates were isolated from citrus fruits and tested for their pathogenicity on Navel orange fruit (Citrus sinensis L.). In this regard, the isolates exhibited three different levels of virulence. The isolates were identified morphologically using malt extract agar (MEA) and Czapek yeast extract agar (CYA) for colony characteristics, and MEA for microscopic features. Each one of the isolates has the unique features of P. digitatum, especially the largest conidia (6.1-11.9 µm long X 3.2-8 µm width), and phialides (10.1-21.4 µm long X 4-5.1 µm width). In addition, the isolates were identified on a molecular basis using ITS1 and ITS4 primers to confirm morphological identification. Phylogenetic analysis revealed that there was high variance among the isolates, and there were different relations between some of our isolates and other P. digitatum registered strains originating from different countries all over the world. The sequences were submitted to respective GenBank nucleotide databases with accession No. OR198852, OR198853, OR198854, OR198855, OR198856, OR198857, OR198858, OR198859 and OR198860. We also isolated a new P. digitatum (OB15: OR198859) strain with high virulence and rapid sporulation
Показать больше [+] Меньше [-]Assessment of Egyptian Local Sheep and Goat Productivity Under the Environmental Conditions of Coastal Zone of Western Desert and Upper Egypt Полный текст
2023
Taha Abdelsabour | Hussein Mansour | Samir Messad | Manal Elsayed | Adel Aboulnaga | Veronique Alary
Assessment of Egyptian Local Sheep and Goat Productivity Under the Environmental Conditions of Coastal Zone of Western Desert and Upper Egypt Полный текст
2023
Taha Abdelsabour | Hussein Mansour | Samir Messad | Manal Elsayed | Adel Aboulnaga | Veronique Alary
Livestock plays a significant role in food production, income generation, soil quality, transportation and the overall welfare of communities, especially in developing nations. Sheep and goats are vital sources of food, livelihood and urgent financial needs. Researching livestock practices can help optimize production, enhance productivity, and improve market access, ultimately boosting the economic well-being of developing communities. Research is needed to identify climate-resilient livestock practices, develop drought-resistant breeds, and optimize feed management strategies. By enhancing the resilience of livestock systems, research can help safeguard food security and livelihoods in the face of climate change. Climate change, evidenced by temperature changes and increased extreme weather events, negatively impacts traditional farming systems and livestock production by affecting pastures, feeds, water quality and livestock diseases. Breeds of the Coastal Zone of Western Desert (CZWD) such as the Barki sheep and goat as well as the Saidi breed in Upper Egypt (UE), have adapted to these harsh conditions and are crucial for smallholders. However, there's an information gap regarding these breeds' productivity in smallholder systems. This study utilizes the Twelve Month Monitoring (12MO) survey to estimate the productivity and demographic parameters of these breeds from 2018 to 2020.
Показать больше [+] Меньше [-]Assessment of Egyptian Local Sheep and Goat Productivity Under the Environmental Conditions of Coastal Zone of Western Desert and Upper Egypt Полный текст
2023
Abdelsabour, Taha | Mansour, Hussein | Messad, Samir | Elsayed, Manal | Aboulnaga, Adel | Alary, Veronique
Livestock plays a significant role in food production, income generation, soil quality, transportation and the overall welfare of communities, especially in developing nations. Sheep and goats are vital sources of food, livelihood and urgent financial needs. Researching livestock practices can help optimize production, enhance productivity, and improve market access, ultimately boosting the economic well-being of developing communities. Research is needed to identify climate-resilient livestock practices, develop drought-resistant breeds, and optimize feed management strategies. By enhancing the resilience of livestock systems, research can help safeguard food security and livelihoods in the face of climate change. Climate change, evidenced by temperature changes and increased extreme weather events, negatively impacts traditional farming systems and livestock production by affecting pastures, feeds, water quality and livestock diseases. Breeds of the Coastal Zone of Western Desert (CZWD) such as the Barki sheep and goat as well as the Saidi breed in Upper Egypt (UE), have adapted to these harsh conditions and are crucial for smallholders. However, there's an information gap regarding these breeds' productivity in smallholder systems. This study utilizes the Twelve Month Monitoring (12MO) survey to estimate the productivity and demographic parameters of these breeds from 2018 to 2020.
Показать больше [+] Меньше [-]A Theoretical Analysis of the Plate Heat Exchanger's Ideal Operating Conditions for Producing Soybean Milk Полный текст
2023
Omnia Salama | Manal Sorour | Ashrf Anwer | Mostafa Abd El-salam
A Theoretical Analysis of the Plate Heat Exchanger's Ideal Operating Conditions for Producing Soybean Milk Полный текст
2023
Omnia Salama | Manal Sorour | Ashrf Anwer | Mostafa Abd El-salam
Heat exchanger optimization is a very efficient thermal design inquiry. The goal of the research is to determine an optimal channel flow velocity as well as an optimal total plate number based on overall information on the effectiveness of employing chilled water to cool soybean milk. Heat transfer coefficient, Prandtl number, Reynolds number and pressure drop measurements were applied for milk and water. The rheological properties of soybean milk have been investigated at various temperatures (5, 10, 30, 50, 70 and 90 °C). The operational circumstances of PHE in soybean procedures were investigated at different numbers of plates, flow rates and flow velocities. Based on the present study, total substances showed non-Newtonian pseudoplastic conduct in any respect at any temperature and the power law model has a decent correlation. The cost estimation of the plate heat exchanger was observed at the lowest flow velocity of (2.25) m/s and number of plates is 17.
Показать больше [+] Меньше [-]A Theoretical Analysis of the Plate Heat Exchanger's Ideal Operating Conditions for Producing Soybean Milk Полный текст
2023
Salama, Omnia | Sorour, Manal | Anwer, Ashrf | Abd El-salam, Mostafa
Heat exchanger optimization is a very efficient thermal design inquiry. The goal of the research is to determine an optimal channel flow velocity as well as an optimal total plate number based on overall information on the effectiveness of employing chilled water to cool soybean milk. Heat transfer coefficient, Prandtl number, Reynolds number and pressure drop measurements were applied for milk and water. The rheological properties of soybean milk have been investigated at various temperatures (5, 10, 30, 50, 70 and 90 °C). The operational circumstances of PHE in soybean procedures were investigated at different numbers of plates, flow rates and flow velocities. Based on the present study, total substances showed non-Newtonian pseudoplastic conduct in any respect at any temperature and the power law model has a decent correlation. The cost estimation of the plate heat exchanger was observed at the lowest flow velocity of (2.25) m/s and number of plates is 17.
Показать больше [+] Меньше [-]Efficacy of Potassium Fertilizer and Certain Pesticides for the Management of Brinjal Fruit Infection by Shoot Borer [Leucinodes Orbonalis (Guenée)] Полный текст
2023
Sagar bhandari | Bikash kandel | Pramod gyawali | Saurav pokhrel | Sushil ojha | Sarita bhandari
Efficacy of Potassium Fertilizer and Certain Pesticides for the Management of Brinjal Fruit Infection by Shoot Borer [Leucinodes Orbonalis (Guenée)] Полный текст
2023
Sagar bhandari | Bikash kandel | Pramod gyawali | Saurav pokhrel | Sushil ojha | Sarita bhandari
The research aims to evaluate the efficacy of different levels of potassium fertilizer (8 g/plant K2O) as well as the insecticides neemix, spinosad, and imidacloprid at the rates of 6 ml, 0.33 ml, and 0.33 g/L water, respectively, for the management of brinjal fruits and shoot borer. The two-factor randomized complete block design with three replications was used. Significant minimum shoot damage was recorded in the plots treated with imidacloprid (0%), spinosad (1.67%) and neemix (5.0%) at 81 days after planting. Similarly, a significant minimum fruit infestation (2.90%) was recorded with imidacloprid, which was lower than those of spinosad (15.16%) and neemix (19.63%). The application of potassium fertilizer at a rate of 8 g/plant showed a significant reduction in shoot infestation (2.50%) 81 days after planting. The minimum percentage yield of infested fruit was reported with spinosad (1.03%), which was at par with other insecticidal treatments. Considering the negative impacts of chemical pesticides, neemix 6 ml/L water in combination with potassium fertilizer at 8 g/plant is recommended.
Показать больше [+] Меньше [-]Efficacy of Potassium Fertilizer and Certain Pesticides for the Management of Brinjal Fruit Infection by Shoot Borer [Leucinodes Orbonalis (Guenée)] Полный текст
2023
bhandari, Sagar | kandel, Bikash | gyawali, Pramod | pokhrel, Saurav | ojha, Sushil | bhandari, Sarita
The research aims to evaluate the efficacy of different levels of potassium fertilizer (8 g/plant K2O) as well as the insecticides neemix, spinosad, and imidacloprid at the rates of 6 ml, 0.33 ml, and 0.33 g/L water, respectively, for the management of brinjal fruits and shoot borer. The two-factor randomized complete block design with three replications was used. Significant minimum shoot damage was recorded in the plots treated with imidacloprid (0%), spinosad (1.67%) and neemix (5.0%) at 81 days after planting. Similarly, a significant minimum fruit infestation (2.90%) was recorded with imidacloprid, which was lower than those of spinosad (15.16%) and neemix (19.63%). The application of potassium fertilizer at a rate of 8 g/plant showed a significant reduction in shoot infestation (2.50%) 81 days after planting. The minimum percentage yield of infested fruit was reported with spinosad (1.03%), which was at par with other insecticidal treatments. Considering the negative impacts of chemical pesticides, neemix 6 ml/L water in combination with potassium fertilizer at 8 g/plant is recommended.
Показать больше [+] Меньше [-]Harvesting Microalgae by Chitosan as Sustainable Biopolymer Полный текст
2023
Ola Obyd | Ayman Abou Hadid | Shawky Selim | Tarek El-Arabi
Harvesting Microalgae by Chitosan as Sustainable Biopolymer Полный текст
2023
Ola Obyd | Ayman Abou Hadid | Shawky Selim | Tarek El-Arabi
This study aimed to use the organic polymer, chitosan, as a flocculant for harvesting microalgae Chlorella. sp. Various polymer concentrations were tested. Within three min, 50 mg/L of chitosan showed the highest efficiency (98.9±0.5%), at pH 6.3, while the lowest efficiency (86.7±0.5%) was observed at pH 7.5. Harvesting efficiency in this study was chitosan concentration dependent. The present study showed that chitosan can be a promising and sustainable solution as an organic source and environmentally friendly flocculant. Additionally, it has excellent harvest productivity and requires minimum effort for quick outcomes.
Показать больше [+] Меньше [-]Harvesting Microalgae by Chitosan as Sustainable Biopolymer Полный текст
2023
Obyd, Ola | Abou Hadid, Ayman | Selim, Shawky | El-Arabi, Tarek
This study aimed to use the organic polymer, chitosan, as a flocculant for harvesting microalgae Chlorella. sp. Various polymer concentrations were tested. Within three min, 50 mg/L of chitosan showed the highest efficiency (98.9±0.5%), at pH 6.3, while the lowest efficiency (86.7±0.5%) was observed at pH 7.5. Harvesting efficiency in this study was chitosan concentration dependent. The present study showed that chitosan can be a promising and sustainable solution as an organic source and environmentally friendly flocculant. Additionally, it has excellent harvest productivity and requires minimum effort for quick outcomes.
Показать больше [+] Меньше [-]Biological Adsorption and Desorption of Anionic Congo Red Dye by Nano Composite Polymer Sheets Полный текст
2023
Hagar Magdy | Hekmat Madian | Ahmed Abdelhamid | Notiala Nasser | Mahmoud Hegazi | Ahmed Labena
Biological Adsorption and Desorption of Anionic Congo Red Dye by Nano Composite Polymer Sheets Полный текст
2023
Hagar Magdy | Hekmat Madian | Ahmed Abdelhamid | Notiala Nasser | Mahmoud Hegazi | Ahmed Labena
Congo red (CR) is an anionic dye that is released from various industries and demonstrates a negative effect on the environment and human health. Dye`s elimination from the environment is an important challenge; therefore, the aim of this study was the application of unhydrolyzed sugarcane bagasse wastes, after acid hydrolysis, drying, grinding and including cellulose acetate to form different composite sheets. The composite sheets were applied in the 3Rs (Removal of Congo red using the composite sheet, Recover of the Congo red after removal using ethanol, and Reuse of the composite sheet many times) processes. The Congo red removal efficiency by the composite sheets was optimized using two statistical methods, One Factor at A Time (OFAT) and General Factorial Design. Afterward, isotherms models and kinetics studies were investigated; moreover, the reusability was also examined. The results exhibited that, the Congo red removal efficiency of 76.27 % was achieved for 500 ppm of the dye using the composite sheet with a concentration of 7 g/L; at a pH value of 7.0 and a contact time of 240 min. Interestingly, the composite sheets have been applied for 3Rs (Removal, Recovery, Reuse) cycles 5 times.
Показать больше [+] Меньше [-]Biological Adsorption and Desorption of Anionic Congo Red Dye by Nano Composite Polymer Sheets Полный текст
2023
Magdy, Hagar | Madian, Hekmat | Abdelhamid, Ahmed | Nasser, Notiala | Hegazi, Mahmoud | Labena, Ahmed
Congo red (CR) is an anionic dye that is released from various industries and demonstrates a negative effect on the environment and human health. Dye`s elimination from the environment is an important challenge; therefore, the aim of this study was the application of unhydrolyzed sugarcane bagasse wastes, after acid hydrolysis, drying, grinding and including cellulose acetate to form different composite sheets. The composite sheets were applied in the 3Rs (Removal of Congo red using the composite sheet, Recover of the Congo red after removal using ethanol, and Reuse of the composite sheet many times) processes. The Congo red removal efficiency by the composite sheets was optimized using two statistical methods, One Factor at A Time (OFAT) and General Factorial Design. Afterward, isotherms models and kinetics studies were investigated; moreover, the reusability was also examined. The results exhibited that, the Congo red removal efficiency of 76.27 % was achieved for 500 ppm of the dye using the composite sheet with a concentration of 7 g/L; at a pH value of 7.0 and a contact time of 240 min. Interestingly, the composite sheets have been applied for 3Rs (Removal, Recovery, Reuse) cycles 5 times.
Показать больше [+] Меньше [-]Effect of Gamma Radiation on Ephedrine and Pseudoephedrine in Callus and Suspension Cultures of Ephedra alata Полный текст
2023
Naglaa Abdelsalam | mona Abd El-Aal | mohamed Aboul Fotouh | mohamed Reda | Ibrahim Safe
Effect of Gamma Radiation on Ephedrine and Pseudoephedrine in Callus and Suspension Cultures of Ephedra alata Полный текст
2023
Naglaa Abdelsalam | mona Abd El-Aal | mohamed Aboul Fotouh | mohamed Reda | Ibrahim Safe
Ephedra is the most widely and largest widespread genus in the Gnetopsida, a subgroup of the gymnosperms. This research was done to find out how gamma radiation affected the in vitro growth of callus and suspension cultures. In addition, the effect of gamma rays (γ-rays) on the ephedrine and pseudoephedrine concentrations was evaluated through HPLC analysis. The cell suspension and callus cultures were formed on Murashige and Skoog's basal medium (MS) supplemented with 0.5 mg/l kinetin (Kn), and 3 mg/l of 2, 4-dichlorophenoxy acetic acid (2,4-D) and exposed to variable gamma radiation doses (0, 5, 10, 15, 20, 25 and 30 Gray). Highly significant differences in both fresh and dry callus weights were recorded due to the effect of gamma rays (γ-rays). The findings demonstrated that in the cell suspension and callus cultures, 15 Gray achieved the highest fresh and dry weights when compared to the control. Similarly, the highest concentrations of ephedrine and pseudoephedrine were found in suspension and callus cultures compared to control at 15 Gray.
Показать больше [+] Меньше [-]Effect of Gamma Radiation on Ephedrine and Pseudoephedrine in Callus and Suspension Cultures of Ephedra alata Полный текст
2023
Abdelsalam, Naglaa | Abd El-Aal, mona | Aboul Fotouh, mohamed | Reda, mohamed | Safe, Ibrahim
Ephedra is the most widely and largest widespread genus in the Gnetopsida, a subgroup of the gymnosperms. This research was done to find out how gamma radiation affected the in vitro growth of callus and suspension cultures. In addition, the effect of gamma rays (γ-rays) on the ephedrine and pseudoephedrine concentrations was evaluated through HPLC analysis. The cell suspension and callus cultures were formed on Murashige and Skoog's basal medium (MS) supplemented with 0.5 mg/l kinetin (Kn), and 3 mg/l of 2, 4-dichlorophenoxy acetic acid (2,4-D) and exposed to variable gamma radiation doses (0, 5, 10, 15, 20, 25 and 30 Gray). Highly significant differences in both fresh and dry callus weights were recorded due to the effect of gamma rays (γ-rays). The findings demonstrated that in the cell suspension and callus cultures, 15 Gray achieved the highest fresh and dry weights when compared to the control. Similarly, the highest concentrations of ephedrine and pseudoephedrine were found in suspension and callus cultures compared to control at 15 Gray.
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