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The Potential Efficiency of Lactobacillus farraginis Isolated from Ruminants with a View to be Used as Animal Probiotics Полный текст
2023
Mona Zayed | Dalia Abd El-Fattah | Rateb Abbas | N. El-Bordeny | Sherif Edris
The Potential Efficiency of Lactobacillus farraginis Isolated from Ruminants with a View to be Used as Animal Probiotics Полный текст
2023
Mona Zayed | Dalia Abd El-Fattah | Rateb Abbas | N. El-Bordeny | Sherif Edris
Probiotics refer to microorganisms that exhibit a beneficial effect on the animals’ health through intestinal microbial balance. This investigation intends to identify and define the probiotic characteristics of Lactobacillus strains isolated from the digestive systems of ruminants. Bacterial strains were isolated purified and characterized based on morphological and biochemical characteristics. The isolates were identified using the 16S rRNA gene partial sequencing method. Most of the strains exhibited a decrease in the growth by increasing the concentration of the bile salt, NaCl, increasing the temperature over 37oC, and moving toward neutrality and alkalinity of the media. Strain Lactobacillus farraginis MD_A11 revealed the lowest decrease in the growth percentage when subjected to different bile salt concentrations of 5.96, 6.61, 6.85, 7.40, 7.53, and 7.64%, NaCl% concentrations of 1.40, 5.62, 6.48, 7.36, 7.39, 7.41, 7.42, 7.28, and 13.76%, raising the temperature over 37oC being 4.19 & 3.945%, different pH levels as compared to control, and it recorded the lowest medium pH after the third day being 4.20 with titrable acidity of 0.32%. Strain Lactobacillus farraginis MD_A11 could be recommended as a probiotic feed additive for ruminants to improve their growth performance and productivity.
Показать больше [+] Меньше [-]The Potential Efficiency of Lactobacillus farraginis Isolated from Ruminants with a View to be Used as Animal Probiotics Полный текст
2023
Zayed, Mona | Abd El-Fattah, Dalia | Abbas, Rateb | El-Bordeny, N.
Probiotics refer to the microorganisms that exhibit a beneficial effect on the animals’ health through intestinal microbial balance. This investigation intends to identify and define the probiotic characteristics of Lactobacillus strains isolated from the digestive systems of ruminants. Bacterial strains were isolated purified and characterized based on morphological and biochemical characteristics. The isolates were identified using the 16S rRNA gene partial sequencing method. Most of the strains exhibited a decrease in the growth by increasing the concentration of the bile salt, NaCl, increasing the temperature over 37˚C, and moving toward neutrality and alkalinity of the media. Strain Lactobacillus farraginis MD_A11 revealed the lowest de-crease in the growth percentage when subjected to different bile salt concentrations of 5.96, 6.61, 6.85, 7.40, 7.53, and 7.64%, NaCl% concentrations of 1.40, 5.62, 6.48, 7.36, 7.39, 7.41, 7.42, 7.28, and 13.76%, raising the temperature over 37oC being 4.19 & 3.945%, different pH levels as compared to control, and it recorded the lowest medium pH after the third day being 4.20 with titrable acidity of 0.32%. Strain Lactobacillus farraginis MD_A11 could be recommended as a probiotic feed additive for ruminants to improve their growth performance and productivity.
Показать больше [+] Меньше [-]Description of Leafhopper, Hebata (Alboneurasca) decipiens (Paoli, 1930) and Its Transmitting Phytoplasma Infesting Sugar Beet Plants Полный текст
2023
Heba Ibrahim | Azza Abdelrahman Emam | Ahmed Bondok | Magdy Ahmed | Amro Abd-Elraheem
Description of Leafhopper, Hebata (Alboneurasca) decipiens (Paoli, 1930) and Its Transmitting Phytoplasma Infesting Sugar Beet Plants Полный текст
2023
Heba Ibrahim | Azza Abdelrahman Emam | Ahmed Bondok | Magdy Ahmed | Amro Abd-Elraheem
Sugar beet (Beta vulgaris L.) is the second sugar crop for sugar production after sugar cane worldwide. This plant is subjected to the infestation of several insect pests that cause considerable damage, especially leafhoppers, that can transmit phytopathogens (e.g., phytoplasma). In this work, we identified and described the leafhopper, Hebata (Alboneurasca) decipiens, in addition to the detection and molecular identification of Candidatus Phytoplasma that was transmitted by this leafhopper on sugar beet plants from naturally infected sugar beet plants to an uninfected one. The 16S rDNA gene from phytoplasma was amplified by a nested polymerase chain reaction (PCR) assay and directly sequenced using specific primer pairs DNA fragments. The resulting sequences were analyzed and compared with another phytoplasma sequence available at GenBank sequences performing BLAST using DNAMAN.
Показать больше [+] Меньше [-]Description of Leafhopper, Hebata (Alboneurasca) decipiens (Paoli, 1930) and Its Transmitting Phytoplasma Infesting Sugar Beet Plants Полный текст
2023
Ibrahim, Heba | Abdelrahman Emam, Azza | Bondok, Ahmed | Ahmed, Magdy | Abd-Elraheem, Amro
The second sugar crop of sugar production after sugar cane world-wide is sugar beet (Beta vulgaris L.). This plant is subjected to the infestation of several insect pests that cause considerable damage, especially leafhoppers, that can transmit phytopathogens (e.g., phytoplasma). In this work, we identified and described the leafhopper, Hebata (Alboneurasca) decipiens, in addition to detection and molecular identification of Candidatus Phyto-plasma that was transmitted by this leafhopper on sugar beet plants from naturally infected sugar beet plants to an uninfected one. The 16S rDNA gene from phytoplasma was amplified by a nested polymerase chain reaction (PCR) assay and directly sequenced using specific primer pairs DNA fragments. The resulting sequences were analyzed and compared with another phytoplasma sequence available at GenBank sequences performing BLAST using DNAMAN.
Показать больше [+] Меньше [-]Effect of Rumen-Protected Choline Supplementation on Growth Performance of Fattening Calves Полный текст
2023
Abdelrahman Amin | Ahmed Marwan | Fouad Abdelaziz | Nasr Elbordiny
Effect of Rumen-Protected Choline Supplementation on Growth Performance of Fattening Calves Полный текст
2023
Abdelrahman Amin | Ahmed Marwan | Fouad Abdelaziz | Nasr Elbordiny
This study aimed to evaluate the influence of choline chloride supplementation on the growth performance of feedlot calves. Thirty-two crossbred calves with a mean initial body weight of 305.71±4.09 Kg were divided into three groups of 10, 11 and 11 animals, randomly. The animals were distributed to one of three dietary treatments, control, choline15 and choline 30 (the rations supplemented with 0, 15 and 30 g choline chloride, respectively). The animals were fed on concentrate feed at a rate of about 2.15% of their live body weight and rice straw open access. Supplementing animal ration with choline chloride did not significantly affect all nutrients' digestibility and feeding value. The animals supplemented with 30 g choline chloride recorded significantly higher (P=0.037) total serum protein concentration compared to the other groups. The group received a ration supplemented with 30 g of choline chloride and recorded significantly higher final weight, total gain, and mean daily gain. Data showed significant improvement in TDN, CP and DCP conversion for the animal received ration supplemented with 30 g choline chloride compared to the control group. In conclusion, supplementing fattening calves' ration with rumen-protected choline could improve average daily gain and feed conversion.
Показать больше [+] Меньше [-]Effect of Rumen-Protected Choline Supplementation on Growth Performance of Fattening Calves Полный текст
2023
Amin, Abdelrahman | marwan, Ahmed | Abdelaziz, Fouad | Elbordiny, Nasr
This study aimed to evaluate the influence of choline chloride supplementation on the growth performance of feedlot calves. Thirty-two crossbred calves with a mean initial body weight of 305.71 ±4.09 Kg were divided into three groups of 10, 11 and 11 animals, randomly. The animals were distributed to one of three dietary treatments, control, choline15 and choline 30 (the rations supplemented with 0, 15 and 30 g choline chloride, respectively). The animals were fed on concentrate feed at a rate of about 2.15% of their live body weight and rice straw open access. Supplementing animal ration with choline chloride did not significantly affect all nutrients digestibility and feeding value. The animals supplemented with 30 g choline chloride recorded significantly higher (P=0.037) total serum protein concentration compared to the other groups. The group received a ration supplemented with 30 g choline chloride and recorded significantly higher final weight, total gain, and mean daily gain. Data showed significant improvement in TDN, CP, and DCP conversion for the animal received ration supplemented with 30 g choline chloride compared to the control group. In conclusion, supplementing fattening calves' ration with rumen-protected choline could improve average daily gain and feed conversion.
Показать больше [+] Меньше [-]Effect of Pruning Levels on Yield and Fruit Quality of Melody (Blagratwo) Seedless Table Grape Cultivar Полный текст
2023
Ali Ali | Nazmy Abdle Hamid | Amin shaddad | Mohamed Nasser
Effect of Pruning Levels on Yield and Fruit Quality of Melody (Blagratwo) Seedless Table Grape Cultivar Полный текст
2023
Ali Ali | Nazmy Abdle Hamid | Amin shaddad | Mohamed Nasser
Three years old uniform vines for cv. "Melody syn. Blagratwo" seedless table grape were selected, and three levels of bud load were trimmed, namely 60, 72, and 78 buds/ vine. The findings revealed that vegetative growth traits, characteristics of yield and bunch quality were significantly affected by all different pruning levels of cane length in both seasons of this study. Highest bud load vines (78 buds/vine) produced the greatest yield and cluster number but had a negative effect on the percentage of bud behavior, shoot thickness, shoot length, weight of annual pruning (Kg/vine), cluster and berry quality parameters. In contrast, lowest vine bud load (78 buds/vine) had a better bud behavior, vegetative growth traits, cluster characteristics and berry characteristics but both bunch numbers and yield were reduced. In addition, vines pruned with bud load of 72 buds per vine significantly showed the best outcomes, since it successfully struck a balance be-tween features of vegetative growth, yield, cluster and berry physical and quality attributes in both seasons.
Показать больше [+] Меньше [-]Effect of Pruning Levels on Yield and Fruit Quality of Melody (Blagratwo) Seedless Table Grape Cultivar Полный текст
2023
Ali, Ali | Abdle Hamid, Nazmy | shaddad, Amin | Nasser, Mohamed
Three years old uniform vines for cv. "Melody syn. Blagratwo" seedless table grape were selected, and three levels of bud load were trimmed, namely 60, 72, and 78 buds/ vine. The findings revealed that vegetative growth traits, characteristics of yield and bunch quality were significantly affected by all different pruning levels of cane length in both seasons of this study. Highest bud load vines (78 buds/vine) produced the greatest yield and cluster number but had a negative effect on the percentage of bud behavior, shoot thickness, shoot length, weight of annual pruning (Kg/vine), cluster and berry quality parameters. In contrast, lowest vine bud load (78 buds/vine) had a better bud behavior, vegetative growth traits, cluster characteristics and berry characteristics but both bunch numbers and yield were reduced. In addition, vines pruned with bud load of 72 buds per vine significantly showed the best outcomes, since it successfully struck a balance be-tween features of vegetative growth, yield, cluster and berry physical and quality attributes in both seasons.
Показать больше [+] Меньше [-]Phylogenetic Evolution of Egyptian Goat Breeds “Capra Hircus” Based on Mitochondrial DNA D-Loop Sequence Полный текст
2023
Eslam Bherey | Heba Hassan | Adel Ellbadawy | Mahmoud El_Maghraby | Mona Moghazee
Phylogenetic Evolution of Egyptian Goat Breeds “Capra Hircus” Based on Mitochondrial DNA D-Loop Sequence Полный текст
2023
Eslam Bherey | Heba Hassan | Adel Ellbadawy | Mahmoud El_Maghraby | Mona Moghazee
The population genetic and molecular evidence of the Arabian goat breed's phylogenetic standing (Capra hircus) is misunderstood, and little is known about genetic diversity in this species. Despite their importance in producing meat and milk, as well as their capacity to endure difficult conditions with little access to food and water, goats are the most common animal in Arab countries. We investigated the phylogenetic history and demographics of domestic goats by sequencing a hypervariable segment of the mtDNA reference region for Baladi, Demuscous, and Zaraibi breeds, as well as sequences retrieved from 16 Arab countries. Three significantly divergent lineages associated with Africa and the Middle East were identified through phylogenetic analysis of the investigated strains of Egyptian goats' mtDNA D-loop region. All Egyptian goats were allocated to lineage A, which is made up of 445 different haplotypes, including Egyptian and Arabian goats. Diversity in haplotypes and in nucleotides for each Arabian goat population was calculated separately and estimated to be 0.99835 and 0.02576, respectively. Finally, we suggest that all goat lineages descend from a single clan. The ancient world's migration and travel may have influenced goat diversity, implying that goats had multiple matrilineal forebears
Показать больше [+] Меньше [-]Phylogenetic Evolution of Egyptian Goat Breeds “Capra Hircus” Based on Mitochondrial DNA D-Loop Sequence Полный текст
2023
Bherey, Eslam | Hassan, Heba | Ellbadawy, Adel | El_Maghraby, Mahmoud | Moghazee, Mona
The population genetic and molecular evidence of the Arabian goat breed's phylogenetic standing (Capra hircus) is misunderstood, and little is known about genetic diversity in this species. Despite their importance in producing meat and milk, as well as their capacity to endure difficult conditions with little access to food and water, goats are the most common animal in Arab countries. We investigated the phylogenetic history and demographics of domestic goats by sequencing a hypervariable segment of the mtDNA reference region for Baladi, Demuscous, and Zaraibi breeds, as well as sequences retrieved from 16 Arab countries. Three significantly divergent lineages associated with Africa and the Middle East were identified through phylogenetic analysis of the investigated strains of Egyptian goats' mtDNA D-loop region. All Egyptian goats were allocated to lineage A, which is made up of 445 different haplotypes, including Egyptian and Arabian goats. Diversity in haplotypes and in nucleotides for each Arabian goat population was calculated separately and estimated to be 0.99835 and 0.02576, respectively. Finally, we suggest that all goat lineages descend from a single clan. The ancient world's migration and travel may have influenced goat diversity, implying that goats had multiple matrilineal forebears
Показать больше [+] Меньше [-]The Relationship Between Vegetation Type and Population Density-Diversity of Spiders in Certain Vegetable Crops Полный текст
2023
Hamdi Abd El-Karim | Marguerite Rizk
The Relationship Between Vegetation Type and Population Density-Diversity of Spiders in Certain Vegetable Crops Полный текст
2023
Hamdi Abd El-Karim | Marguerite Rizk
The experiment was carried out on three different vegetable crops, Cucumber Cucumis sativus L. (Cucurbitaceae), eggplant Solanum melongena L. (Solanaceae) and okra Abelmoschus esculentus L. (Malvaceae), during summer 2021, to investigate the effect of vegetation type on spider density and diversity. For this study, the pitfall trap method was used to collect spiders from the soil surface to ensure a comprehensive representation of all spider guilds. During this study, 20 species belonging to 6 families were collected. The results showed that a total of 374, 285 and 194 individuals belonging to 18, 17 and 15 species were recorded in okra, cucumber and eggplant fields respectively. Species diversity was greater in okra cultivation. Results also indicated that the Lycosidae family was the most abundant and dominant, representing 71.75%, followed by the Linyphiidae family of 90 individuals representing 10.55% of the whole obtained population. The most dominant species were Wadicosa fidelis 152, 98 and 67 individuals and Pardosa injucunda 111, 69 and 52 individuals in okra, cucumber and eggplant respectively. It is inferred from this study that the type of plant can have an impact on spider communities.
Показать больше [+] Меньше [-]The Relationship Between Vegetation Type and Population Density-Diversity of Spiders in Certain Vegetable Crops Полный текст
2023
Abd El-Karim, Hamdi | Rizk, Marguerite
The experiment was carried out on three different vegetable crops, Cucumber Cucumis sativus L. (Cucurbitaceae), eggplant Solanum melongena L. (Solanaceae) and okra Abelmoschus esculentus L. (Malvaceae), during summer 2021, to investigate the effect of vegetation type on spider density and diversity. For this study, the pitfall trap method was used to collect spiders from the soil surface to ensure a comprehensive representation of all spider guilds. During this study, 20 species belonging to 6 families were collected. The results showed that a total of 374, 285 and 194 individuals belonging to 18, 17 and 15 species were recorded in okra, cucumber and eggplant fields respectively. Species diversity was greater in okra cultivation. Results also indicated that the Lycosidae family was the most abundant and dominant, representing 71.75%, followed by the Linyphiidae family of 90 individuals representing 10.55% of the whole obtained population. The most dominant species were Wadicosa fidelis 152, 98 and 67 individuals and Pardosa injucunda 111, 69 and 52 individuals in okra, cucumber and eggplant respectively. It is inferred from this study that the type of plant can have an impact on spider communities.
Показать больше [+] Меньше [-]Decreasing of Monosaccharide Contents Resulted From Cold- Induced Sweetening in Tetraploid Potato (Solanum tuberosum L.) Using Genome Editing Полный текст
2023
Sally Hassan | Mohamed Abdel-Salam | ayman hanfy | Haytham Abd-Elhalim | Nouh Ahmed | Emad Metry
Decreasing of Monosaccharide Contents Resulted From Cold- Induced Sweetening in Tetraploid Potato (Solanum tuberosum L.) Using Genome Editing Полный текст
2023
Sally Hassan | Mohamed Abdel-Salam | ayman hanfy | Haytham Abd-Elhalim | Nouh Ahmed | Emad Metry
Worldwide, potato (Solanum tuberosum L.) is one of the most essential agro-economically food crops. To ensure its presence throughout the year for food processors and to extend its shelf life, it should be stored in cold temperatures. Despite the benefits of storage at low temperatures, it causes undesirable phenomena; one of them is cold-induced sweetening, which reduces the quality and the commercial value of the potato tubers. In this study, the CRISPR technology ″Clustered Regularly Interspaced Short Palindromic Repeats″ has been used in potato cultivar Desiree via Agrobacterium mediated transformation to edit starch phosphorylase gene that is responsible for starch degradation in cold temperature storage, after regeneration steps. The regenerated plants were used in the screening of genome editing by Sanger sequencing and the ICE program. Screening results showed that Desiree 2 (DE2), Desiree 7 (DE7) and Desiree 27 (DE27) lines have mutations in sgRNA sequence that indicated a significant reduction in reduced sugar contents of tubers after cold storage for 90 and 120 days. The reduction mean values were 29.224, 35.078 and 35.862 in DE2, DE7 and DE27 respectively after 90 days and 83.212, 56.674 and 34.109 after 120 days without visible differences in plant morphology and tubers
Показать больше [+] Меньше [-]Decreasing of Monosaccharide Contents Resulted From Cold- Induced Sweetening in Tetraploid Potato (Solanum tuberosum L.) Using Genome Editing Полный текст
2023
Hassan, Sally | Abdel-Salam, Mohamed | hanfy, ayman | Abd-Elhalim, Haytham | Ahmed, Nouh | Metry, Emad
Worldwide, potato (Solanum tuberosum L.) is one of the most essential agro-economically food crops. To ensure its presence throughout the year for food processors and to extend its shelf life, it should be stored in cold temperatures. Despite the benefits of storage at low temperatures, it causes undesirable phenomena; one of them is cold-induced sweetening, which reduces the quality and the commercial value of the potato tubers. In this study, the CRISPR technology ″Clustered Regularly Interspaced Short Palindromic Repeats″ has been used in potato cultivar Desiree via Agrobacterium mediated transformation to edit starch phosphorylase gene that is responsible for starch degradation in cold temperature storage, after regeneration steps. The regenerated plants were used in the screening of genome editing by Sanger sequencing and the ICE program. Screening results showed that Desiree 2 (DE2), Desiree 7 (DE7) and Desiree 27 (DE27) lines have mutations in sgRNA sequence that indicated a significant reduction in reduced sugar contents of tubers after cold storage for 90 and 120 days. The reduction mean values were 29.224, 35.078 and 35.862 in DE2, DE7 and DE27 respectively after 90 days and 83.212, 56.674 and 34.109 after 120 days without visible differences in plant morphology and tubers
Показать больше [+] Меньше [-]Effect of Gamma Radiation on Ephedrine and Pseudoephedrine in Callus and Suspension Cultures of Ephedra alata Полный текст
2023
Naglaa Abdelsalam | mona Abd El-Aal | mohamed Aboul Fotouh | mohamed Reda | Ibrahim Safe
Effect of Gamma Radiation on Ephedrine and Pseudoephedrine in Callus and Suspension Cultures of Ephedra alata Полный текст
2023
Naglaa Abdelsalam | mona Abd El-Aal | mohamed Aboul Fotouh | mohamed Reda | Ibrahim Safe
Ephedra is the most widely and largest widespread genus in the Gnetopsida, a subgroup of the gymnosperms. This research was done to find out how gamma radiation affected the in vitro growth of callus and suspension cultures. In addition, the effect of gamma rays (γ-rays) on the ephedrine and pseudoephedrine concentrations was evaluated through HPLC analysis. The cell suspension and callus cultures were formed on Murashige and Skoog's basal medium (MS) supplemented with 0.5 mg/l kinetin (Kn), and 3 mg/l of 2, 4-dichlorophenoxy acetic acid (2,4-D) and exposed to variable gamma radiation doses (0, 5, 10, 15, 20, 25 and 30 Gray). Highly significant differences in both fresh and dry callus weights were recorded due to the effect of gamma rays (γ-rays). The findings demonstrated that in the cell suspension and callus cultures, 15 Gray achieved the highest fresh and dry weights when compared to the control. Similarly, the highest concentrations of ephedrine and pseudoephedrine were found in suspension and callus cultures compared to control at 15 Gray.
Показать больше [+] Меньше [-]Effect of Gamma Radiation on Ephedrine and Pseudoephedrine in Callus and Suspension Cultures of Ephedra alata Полный текст
2023
Abdelsalam, Naglaa | Abd El-Aal, mona | Aboul Fotouh, mohamed | Reda, mohamed | Safe, Ibrahim
Ephedra is the most widely and largest widespread genus in the Gnetopsida, a subgroup of the gymnosperms. This research was done to find out how gamma radiation affected the in vitro growth of callus and suspension cultures. In addition, the effect of gamma rays (γ-rays) on the ephedrine and pseudoephedrine concentrations was evaluated through HPLC analysis. The cell suspension and callus cultures were formed on Murashige and Skoog's basal medium (MS) supplemented with 0.5 mg/l kinetin (Kn), and 3 mg/l of 2, 4-dichlorophenoxy acetic acid (2,4-D) and exposed to variable gamma radiation doses (0, 5, 10, 15, 20, 25 and 30 Gray). Highly significant differences in both fresh and dry callus weights were recorded due to the effect of gamma rays (γ-rays). The findings demonstrated that in the cell suspension and callus cultures, 15 Gray achieved the highest fresh and dry weights when compared to the control. Similarly, the highest concentrations of ephedrine and pseudoephedrine were found in suspension and callus cultures compared to control at 15 Gray.
Показать больше [+] Меньше [-]Predicting and Optimizing Tillage Draft Using Artificial Network Technique Полный текст
2023
Yasmin Shehta | Nabil Awady | Abdel-Fadil Kabany | Mohammed Abd-Elwahed | Waleed Elhelew
Predicting and Optimizing Tillage Draft Using Artificial Network Technique Полный текст
2023
Yasmin Shehta | Nabil Awady | Abdel-Fadil Kabany | Mohammed Abd-Elwahed | Waleed Elhelew
Tillage as one of the agricultural practices consumes the largest amount of energy, which reflects on the total production cost. The artificial neural network (ANN) technique was utilized in the current study to opti-mize the performance of the tillage process. The ANN-modeled multilayer perceptron network with a backpropagation learning algorithm and momen-tum term was used by the PYTHON program. The ANN inputs were: the implement type, soil texture, moisture, bulk density, width, speed, and depth. The draught was the output (kN). Five layers composed the ANN model's optimal configuration (13-64-16-4-1). The linear and rectified linear units (ReLU) functions were utilized with hidden layers and the output layer, re-spectively. Momentum term and learning rate were 0.00003 and 0.9 respec-tively. The iteration number was 1000 epochs and stopped at 290 epochs. The coefficient of determination in the test datasets was high (0.92) while the difference between actual and predicted output was low (2.08). Bulk den-sity and depth were the main determinants of the draft. The evaluation of the developed model for chisel, moldboard, and disk plow gave satisfactory re-sults of 0.985, 0.924, and 0.917. In comparison to the ANNs, the regression model's correlation coefficient for predicting draught force was the lowest (0.373).
Показать больше [+] Меньше [-]Predicting and Optimizing Tillage Draft Using Artificial Network Technique Полный текст
2023
Shehta, Yasmin | Awady, Nabil | Kabany, Abdel-Fadil | Abd-Elwahed, Mohammed | Elhelew, Waleed
Machine learning models, Artificial neural network, Tillage performance, Energy needs, draught Tillage as one of the agricultural practices consumes the largest amount of energy, which reflects on the total production cost. The artificial neural network (ANN) technique was utilized in the current study to optimize the performance of the tillage process. The ANN-modeled multilayer perceptron network with a backpropagation learning algorithm and momentum term was used by the PYTHON program. The ANN inputs were the implement type, soil texture, moisture, bulk density, width, speed, and depth. The draught was the output (kN). Five layers composed the ANN model's optimal configuration (13-64-16-4-1). The linear and rectified linear units (ReLU) functions were utilized with hidden layers and the output layer, respectively. Momentum term and learning rate were 0.00003 and 0.9 respectively. The iteration number was 1000 epochs and stopped at 290 epochs. The coefficient of determination in the test datasets was high (0.92) while the difference between actual and predicted output was low (2.08). Bulk density and depth were the main determinants of the draft. The evaluation of the developed model for chisel, moldboard, and disk plow gave satisfactory results of 0.985, 0.924, and 0.917. In comparison to the ANNs, the regression model's correlation coefficient for predicting draught force was the lowest (0.373).
Показать больше [+] Меньше [-]Partial Gap-Filling of the Nile Tilapia (Oreochromis niloticus) Draft Genome Полный текст
2023
Hala Zoghly | Mohamed Rashed | Mahmoud Magdy
Partial Gap-Filling of the Nile Tilapia (Oreochromis niloticus) Draft Genome Полный текст
2023
Hala Zoghly | Mohamed Rashed | Mahmoud Magdy
Nile tilapia is a freshwater fish of African origin, with productive and economic characteristics affecting global fish markets. The Oreochromis niloticus reference genome has a diploid set of 22 linkage groups (LGs) in addition to ungrouped sequences. A total of 42,622 genes have been identified, but 550 genomic gaps may include more. Our study focused on some of these genomic gaps, where appropriate primers were designed and then multiplied through the polymerase chain reaction (PCR) technique. From local samples, DNA was extracted and amplified with the new primers. Successful amplicons were sequenced and annotated using available bioinformatics tools. Five assessed sequences were annotated, of which three were newly predicted in O. niloticus, as mucin 1-like, and KLR genes, and SATB region. The other two sequences belonged to grid2 and trpm7 which were previously predicted. Although individual attempts to identify genomic gaps are not feasible in filling the large lack of information on the Nile tilapia genome, a good number and valuable new information has been reached. However, the following plan showed many technical problems, more time, effort and cost that could be avoided by suggesting the application of another technique, such as whole-genome sequencing, mapping, and assembly.
Показать больше [+] Меньше [-]Partial Gap-Filling of the Nile Tilapia Oreochromis niloticus Draft Genome Полный текст
2023
Zoghly, Hala | Rashed, Mohamed | Magdy, Mahmoud
Nile tilapia is a freshwater fish of African origin, with productive and economical characteristics affecting global fish markets. The Oreochromis niloticus reference genome has a diploid set of 22 linkage groups (LGs) in addition to ungrouped sequences. A total of 42,622 genes have been identified, but 550 genomic gaps may include more. Our study focused on some of these genomic gaps, where appropriate primers were designed and then multiplied through the polymerase chain reaction (PCR) technique. From local samples, DNA was extracted and amplified with the new primers. Successful amplicons were sequenced and annotated using available bioinformatics tools. Five assessed sequences were annotated, of which three were newly predicted in O. niloticus, as mucin 1-like, and KLR genes, and SATB region. The other two sequences belonged to grid2 and trpm7 which were previously predicted. Although individual attempts to identify genomic gaps are not feasible in filling the large lack of information on the Nile tilapia genome, a good number and valuable new information has been reached. How-ever, the following plan showed many technical problems, more time, effort, and cost that could be avoided by suggesting the application of another technique, such as whole-genome sequencing, mapping, and assembly.
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