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Effect of neem and willow aqueous extracts on fusarium wilt disease in tomato seedlins 1-induction of antioxidant defensive enzymes
2011
Farag, H.R.M. | Abdou, Z.A. | Salama, D.A. | Ibrahim, M.A.R.
. The effects of neem (Azadirachta indica) and willow (Salix babylonica) aqueous extracts on fusarium wilt disease in tomato seedlings were investigated. Four weeks old tomato seedlings were treated with 10% of either neem and willow aqueous extracts and then infected with Fusarium oxysporum after 4 days of treatment. The results showed that the percentage of disease incidence was increased in non treated tomato seedlings in time dependent manner and reached the maximum level (65%) after 6 weeks of infection. Treatments of tomato plants with neem and willow aqueous extracts reduced the percentage of disease incidence to the level of 25.5% and 27.8% after 6 weeks of infection respectively. The results show that infection of tomato seedling with Fusarium oxysporum led to many morphological and biochemical changes including, reducing the growth of tomato shoot and root, increasing the level of lipid peroxidation and marked increase in the activities of antioxidant defensive enzyme I.e. POX, CAT, and SOD. Treatment with neem and willow aqueous extracts significantly exhibited a growth promotion of tomato shoot and root in infected or non infected seedling. Moreover, application of neem and willow aqueous extracts with fusarium, significantly reduce the level of lipid peroxidation and induce high activities of antioxidant defensive enzymes after 3 and 7 days of infection. Electrophoretic pattern of POX demonstrated that Fusarium oxysporum caused up regulation of several POX isoenzymes. It could be concluded that neem and willow aqueous extracts reduced the disease incidence of fusarium wilt in tomato seedlings by increasing the activities of antioxidant defensive enzymes and decreasing the level of lipid peroxidation.
Показать больше [+] Меньше [-]Cellulase productrion two fungal strains isolated from Taif in Saudi Arabia
2011
Nasr, S.A. | Hussein, N.A. | Abuo zaid, A.A. | Al-Salemi, F.A
Among 17 fungal isolates isolated from soil of El-hawia, El-hada, El-kaym and Karwa in Taif governorate in Saudi Arabia, two isolates showed high efficacy in producing cellulases enzymes. They were identified to be Altemaria altemata and Aspergillus wentii. Some factors such as carbon and wheat bran as a raw material, nitrogen, pH and incubation temperature were investigated. Results indicated that glucose and cellulose were the most effective as a carbon source while, urea was the best nitrogen source for cellulases production. Initial pH 5.0 and incubation temperatures at 25 or 35°C achieved high cellulases production.
Показать больше [+] Меньше [-]Microbial production of thermoalkaliphilic enzymes from El-Khorma governorate for application in biodetergent technology
2011
Bayoumi, R.A. | Atta, H.M. | El-Sehrawey, M.H. | Selim, Sh.M.
Detergents are an undetectable source of pollution, which hidden in most of our daily activities. Detergents could cause harmful effects before they are completely degraded. It is wise to reduce the amount of detergents that usually used by invention new bio-friendly formula contains efficient enzymes such as protease. Screening studies were carried out for one hundred and fifty bacterial isolates with respect to their ability to produce protease(s), after growing on slaughter house wastes (SHW) isolated from EI-Khorma governorate,Taif, Kingdom of Saudi Arabia (KSA) at 55°C, and pH 9. The most potent thermophilic bacterial isolate concerning of alkaline thermostable protease(s) production was identified as Bacillus licheniformis EGT50. Alkaline thermostable proteases productivity by the most potent bacterial isolate was affected by substrate concentrations (solid substrate), carbon source, nitrogen source, amino acid supplements, incubation temperature, incubation period, and inoculum size. Maximum both enzymes production by B. licheniformis EGT50 was obtained on SHW concentrations, 7.5 %; galactose; diammonium hydrogen phosphate; arginine at 55°C for 72 h. when inoculated by 0.5 ml. The protease production under all optimal conditions was increased many folds from 563.68 to 17825 U/ml (31 fold). The purification fold of B. licheniformis EGT50 alkaline thermostable protease increased to 394.7 after applying Sephadex G200 column chromatography techniques. The enzyme productivity of protease has been determined and the result proved the possibility to use the crude and purified enzymes in biodetergent technology.
Показать больше [+] Меньше [-]Analytic study of short fiber content in Egyptian cotton
2011
Ebado, E.A. | Hassan, R.M.
The experiment was conducted through two successive seasons of 2008 and 2009 at El Bosaily farm, EI-Behira governorate at the North Coastal of the Nile Delta, in Egypt. This work aimed to study the effect of three green-house covers (polyethylene sheet, white and black net) and three irrigation levels 180%, 100% and 120% of the potential evapotranspiration estimated according to class A pan equation (ET,)], applied by drip irrigation system, on plant growth and crop yield of Cucumber (Cucumis sativus L.. cv. Reda F1). The experimental design was split- plot with three replicates. The results showed that white net green-house cover optimized growth and yield of cucumber plant. White net cover treatment recorded the highest vegetative growth (plant height, number of leaves, total leaves area, total fresh and dry weights), and significantly increased total yield. The highest vegetative growth was obtained by 100% ETo compared to 80 and 120% ETo treatments. The interaction among water treatment and green-house cover indicated the highest vegetative growth and crop yield were obtained under white net cover with 100% ET,.
Показать больше [+] Меньше [-]Physiological response of strawberry grown in root-knot nematode infested soil under different safety control applications
2011
Mahdy, M.E. | Midan, S.A.
The evaluate some safety treatments including: mulching, tunnels, natural yeast extract, antioxidant and two rhizobacteria Serratia marcescens and Pseudomonas fluorescens towards root-knot nematode Meloidogyne spp. on strawberry plant grown in naturally infested soil and consequently their probable effects on growth parameters, chemical components and yield of strawberry compared to untreated control. Results of this study confirmed that all used treatments, significantly reduced all related nematode parameters i.e. number of galls/root system; number of egg masses/root system as well as number of juveniles/250 g soil (either after 2 or 4 months) when compared to plants grown in the naturally infested soil only. The highest percentage of reduction in all related nematode parameters were noticed after 4 months. Mulching, S. marcescens, P. fluorescens and tunnels, played an important role in reducing the percentage of nematode parameters. Mulching and S. marcescens reduced the percentage of gall numbers/root system by 77%, followed by tunnels by 75% and P. fluorescens by 70%. S. marcescens reduced the percentage of egg masses/root system by 80% followed by P. fluorescens by 78% and mulching by 76%. Results indicated that the soil treated with mulching, also significantly reduced the percent age of juvenile numbers/250 g soil by 79% followed by S. marcescens by 73%. . Results revealed that all previous treatments significantly increased the early and total yield. Our results confirmed that, the rhizobacterium S. marcescens came in the first rank as a promoter of all related plant growth characters, followed by tunnels and mulching treatments. Results confirmed that also the antioxidants, are considered the effective one in enhancing the activities of peroxidase and phenoloxidase as well as T:S.S% and V.C followed by the natural yeast extract.
Показать больше [+] Меньше [-]RESPONSE OF RICE AND ASSOCIATED WEEDS TO BIOREGULATOR (BIO HORM) SPRAY AND WEED MANAGEMENT IN SALINITY SOIL Полный текст
2010
, A.F.; Abou-Hadid | .; M.A. Medany | ; M. Abdrabbo | ; M.K. Hassanein | ; A.A. Farag | S.M. Abolmaaty | ; A.A. Khalil | R.M.H Tagour | G.M Abd El-Hamed | I.M El-Metwally
Two field experiments were conducted during the two successive seasons of 2007 and 2008 at the Agricultural Experimental Station of Agricultural Research Center, at EL-Serw, Damietta Gover-norate Egypt, to study the effect of the bio horm and weed control treatments (Bentazon, Py-razosoulfuron, Fenoxaprop, Penoxsulam, Bentazon + Fenoxaprop, Bentazon + Penoxsulam, Pyrazosoulfuron + Fenoxaprop, Pyrazosoulfuron + Penoxsulam, twice hand weeding and un- weeded check ) on growth, yield, its components and NPK percentage in grains of direct seeded rice as well as associated weeds in salinity soil. Results illustrated that appli-cation of bio horm increased fresh and dry weight of total weeds. Spraying bio horm after 50 days from sowing (DFS) markedly increased rice growth characters (plant height, number of productive till-ers, fresh and dry weight of rice plants), yield and yield components (panicle length, number of pani-cles/m2, weight of 1000 grain, grain and straw yield) and N, P and K% of grain rice, as compared with untreated treatment. All weed control treat-ments caused significant reduction on number, fresh and dry weight of total weeds/m2 after 60 DFS in both seasons. Pyrazosoulfuron treatment gave the highest decrease in total number of weeds followed by Pyrazosoulfuron + Penoxsulam and Pyrazosoulfuron + Fenoxaprop treatments. Pyrazosoulfuron + Fenoxaprop treatment gave the highest decrease in total fresh weight of weeds followed by Bentazon + Penoxsulam, Pyrazosoul-furon and Pyrazosoulfuron + Penoxsulam, respec-tively in the first season. While, in the second sea-son Pyrazosoulfuron treatment gave the lowest values of total fresh weight of weeds. Meanwhile, Bentazon + Penoxsulam was more effective than other treatments in reducing the dry weight of total weeds in both seasons. All herbicidal treatments and hand weeding increased significantly growth, yield, yield components and chemical composition of grain rice. Bentazon + Penoxsulam was superior treatment for increasing number of productive tillers, number of panicles / m2, 1000- grain weight and grain and straw yield as well as NPK percent-age of rice grains in both seasons. While, the same treatment Bentazon + Penoxsulam recorded the highest values of plant height, fresh and dry weight of whole plant and panical length in the first season. While, Pyrazosoulfuron + Penoxsulam gave the highest increment in plant height, fresh and dry weight of whole plant and straw yield in the second season. The interaction between bio horm and weed management treatments had significant effect on total fresh and dry weight of weeds, num-ber of productive tillers and 1000 grain weight. Pyrazosoulfuron produced the maximum values of number of productive tillers when bio horm was used in the second season, while, application of Bentazon + Penoxsulam gave the highest values of 1000 grain weight when bio horm was used in the second season. It could be concluded that using the bioregulator (bio horm) resulted in incre-ment of growth and productivity of rice crop under salinity soils. Using the combinations of herbicides caused more reduction of weeds and increased rice grain yield.
Показать больше [+] Меньше [-]COMPARATIVE STUDIES BETWEEN SOME SESAME CULTIVARS GROWN UNDER DIFFERENT EDAPHIC AND CLIMATIC CONDITIONS Полный текст
2010
Two successive field experiments were carried out during two seasons (2006 and 2007) at three Agricultural Experimental stations of Desert Research Center (DRC) in three locations: El-Maghara, El-Wadi El-Gedeed and Maryout to evaluate variation, mean performance and stability parameters of four diverse sesame cultivars. Also, study the oil content, physical and chemical properties, fatty acids composition of oil and proteins patterns in seeds of the tested cultivars (Shandaweel 3, Toushki 1, Giza 32 and Taka 3). The results obtained could be summarized in the following categories:- Wide range of variability between locations and high genotypic differences were detected. The genotype x environment interaction was significant and a major portion of this was accounted by the deviation among source of variations. Hence, the genotypes tested had a wide diversity and ranked differently among locations and seasons. The average plant height, number of branches/plant, number of capsules/plant, 1000-seed weight, grain yield/plant, grain yield/feddan and oil yield/feddan were recorded under El-Wadi El-Gedeed, El-Maghara and Maryout locations. Toushki 1 recorded the highest values across different locations for Number of capsules/plant (46.21), number of seeds/plant (47.27), seed yield/plant (14.76 g.), seed yield/feddan (333.84 kg.) and oil yield/feddan (168.56 kg.). El-Wadi El-Gedeed location is considered the best location for most studied traits except for number of branches/ plant. On the other hand, Maryout location (calcareous 34% CaCo3) recorded the lowest values for all traits under study; such findings may be due to the differences between soil and irrigation water types. Stability parameters were fitting together in determining the stable genotypes Toushki1, Shandaweel 3, Giza32 and Taka3 overall tested environments. Genotypes varied in their calculated bi values as well as S2di . Toushki 1 is the most stable genotype across different environments and for all traits under study except for number of capsules/plant. However, genotypes produced higher yield such as Toushki 1 and Shandaweel 3 showed below average stability and were well adapted for El-Wadi El-Gedeed conditions. Oil content was increased while protein content was decreased significantly in seeds of Shandaweel3 and Toushki1 under El-Wadi El-Gedeed location. However, acid value was decreased for all cultivars except Taka 3 under El-Wadi El-Gedeed location at zero and 6 months of storage. On the other hand, the oil of all sesame cultivars which grown under El-Wadi El-Gedeed location had a higher iodine value than oil of the same cultivars under El-Maghara location. In
Показать больше [+] Меньше [-]CONSUMPTION AND UTILIZATION OF CASTOR BEAN LEAVES TREATED WITH NEEM SEED KERNEL EXTRACTS BY THE COTTON LEAF WORM LARVAE, Spodoptera littoralis (Boisd.) Полный текст
2010
R.M.H Tagour | , H.A. Gomaa | G.M Abd El-Hamed
Larva of cotton leaf worm, Spodoptera littoralis ate and digested an average of 13.79 and 10.82g, respectively of untreated castor bean leaves during its whole sixth instar. These amounts significantly reduced when larvae were fed on leaves treated with different concentrations of neem seed kernel methanolic extract. The reduction was concentration dependent, i.e., an increase in neem extract concentration caused an obvious decrease in the amount of ingested or digested leaves. The consumption and digestion indices are always negative indicating that treated castor bean leaves are not preferred by S. littoralis larvae for feeding. These indices decreased as the concentrations of neem extract increased. Approximate digestibility in S. littoralis sixth instar larvae averaged 78.50%. This percentage insignificantly fluctuated between 77.71 and 81.08% when larvae were fed on castor bean leaves treated with different concentrations of neem extract. Efficiencies of conversion of ingested and digested food to body substances (ECI and ECD) are an overall indicators for ability of S. littoralis larvae to utilize ingested and digested food for growth and food storage in the insect. Such values depend upon the food digestibility, its nutritional values and the ingestion rates. ECI and ECD in larvae fed on untreated leavesreached 22.25 and 28.25%, respectively. These values decreased after feeding the larvae on treated leaves. The decrease was gradually occurred as the concentration of neem extract increased to reach the minimum value at the highest concentration (0.025%).
Показать больше [+] Меньше [-]PROTECTIVE EFFECT OF CINNAMON, CLOVE AND GINGER SPICCES OR THEIR ESSENTIAL OILS ON OXIDATIVE STRESS OF STREPTOZOTOCIN-INDUCED DIABETIC RATS Полный текст
2010
Naglaa Hassanen
In an attempt for utilization of some common spices, cinnamon bark, clove bud and ginger rhi-zom are popular implementations because of their flavoring and antioxidative activity, which mainly comes from polyphenols. The aim of the study was to investigate the effect of spices or their essential oils compared with Diamicron30MR (60mg /100g diet) on the occurrence of oxidative stress in serum of induced diabetic rats by measuring the extent of oxidative damage as well as the status of the anti-oxidant defense system. Albino rats weighing 150 ± 5 g were injected with STZ (50 mg/kg) intraperi-toneally for induction of diabetes mellitus. Rats were divided into 17groups (each of 8 rats) of non-diabetic ,diabetic non-treated and diabetic treated rats with spice powders or their essential oils and mixtures. After 8 weeks, the diabetic rats fed on spices or their essential oils significantly decreased levels of blood glucose and significantly increased insulin level. The treatment also resulted in a sig-nificant improvement in lipid profile, liver functions and kidney functions. However, a significantly in-crement in the activities of glutathione peroxidase (GPH-Px) and concentration of glutathione (GSH) were observed in blood of diabetic rats treated with all of the essential oils. The treated groups showed a significant decrement in thiobarbituric acid reac-tive substances (TBARS) in serum. Since the study of induction of the redox enzymes is considered to be a reliable marker for evaluating the antiperoxi-dative efficacy of the spices, these findings sug-gest a possible antiperoxidative role derived from such essential oils. Treatment with spices or their essential oils reduces the hepatic, renal, pancreat-ic and cardiac histopathological abnormalities as-sociated with STZ – induced diabetes mellitus
Показать больше [+] Меньше [-]INFLUENCE OF MOLASS APPLICATION ON SOME SOIL HYDRAULIC CHARACTERISTICS AND INFILTRATION RATE, RELATED TO THE SOIL STRUCTURE Полный текст
2010
Ati, Alaa | Younan Taghreed
A laboratory study was performed to evaluate the role of Molass using four levels (C=0, 10, 20 and 40 g kg-1) on the water transport function [namely, penetrability (l), sorptivity (S) and infiltration rate (i)] during transient flow in horizontal infiltration in Sandy loam soil incubated with Molass at 25 ± 2°C and 80% relative moisture content at 33 Kpa. For 60 days. Boltzmann transformation was used to esitmate penetrability by fitting the wet front distance X vs. square root of time (t). Equation of Philip (1957) was used to estimate sorptivity (S) by fitting cumulative depth of water observed (I) vs. . Infiltration rate (i) was calculated using equation. The contact angle (a), soil surface free energy were measured and calculated for all soil treatments. We also studied the effect of Molass on aggregate stability from the values of Mean Wight Diameter (MWD) and Geometric Mean Diameter (GMD). Results showed significant response and decrease in all study water transport functions [(l), (S) and (i)] with distance 30 cm of wetting front advance in end time with increasing the levels of Molass from 0.0 to 40 g kg-1. Value of contact angle increased from 49.54 to 76.17, while the value of soil surface free energy decreased from 135.9 to 76.8 (m N m-1). The addition of Molass played very important role in aggregate stability according to the value of MWD and GMD
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