This study aimed to establishing a protocol for producing in-vitro plants of Rosa Damascene. The culture was conducted at the Commission of Biotechnology in Damascus. Four types of explants were cultured on MS medium and some factors affecting culture were examined. The results showed that no viruses were observed, the lateral buds were superior over other explants, then the lateral microcuttings, after that, the apical micro-cuttings, and, finally, the shoot tips. The highest multiplication rate was observed at the hormonal combinations of (benzyl adenine BA 3mg/l with indole-3-acetic acid  IAA 0.1 mg/l), and the highest elongation average were observed at ( IAA 0.1 mg/l with BA 2-6mg/l) or ( indole-3-butyric acid (IBA) 0.1 with (BA) 5-6 mg/l). The transferring was positively effective. The highest rooting percentage was observed when naphthalene acetic acid NAA or IBA were used. (Berlite: peatmoss, 1:1) was the best growing medium for hardening.

A greenhouse pot experiment was carried out to investigate the effect of salinity and potassium at different levels alone or in various combinations on growth, mineral and proline content in leaves of plantlets of Phoenix dactylifera L. cv. Bartomouda (in vitro production, two years old from acclimatized them). The following treatments were applied: three levels of salinity Na Cl + Ca Cl2 w.w 2:1 (14000, 16000 and 18000 ppm.) and two levels of potassium (2000 and 3000 ppm) in addition to control (no salts or potassium used), salts and potassium were added in the irrigation water. In general, all levels of salinity significantly decreased various growth parameters such as plant height, number of leaves and roots, root length, fresh and dry weights of leaves than that of the control. These parameters were decreased with increasing salinity levels, whereas it, significantly increased Na, Ca and K contents in leaves with high content of proline. The treatment 18000 ppm salts gave the highest significant reduction of the growth parameters, while caused an increase in proline Na, Ca, and K contents compared to control treatment (no salts) . This was true in both seasons. The applications of potassium significantly increased the previous growth parameters as compared with the control treatment (without salts and potassium) the treatment 3000 ppm had the highest results. Moreover the applications of potassium gave high alleviated the negative effects of salt stress, the treatment 3000 ppm gave the best results on the growth parameters of date palm plantlets grown under salinity condition . Regarding the interaction the obtained data revealed that the interaction between treatment 3000 ppm potassium and 14000 salts produced the highest significant results. Generally, from the obtained results it can conclude that the plantlets of date palm produced by tissue culture can be tolerated salt stress by addition of potassium which can significantly ameliorate the harmful effects of salts, positive effects on the growth parameters of the plantlets was showed by potassium applications.

Two successive field experiments during two seasons of 2008 and 2009, were carried out to evaluate some safety treatments including: mulching, tunnels, natural yeast extract, antioxidant and two rhizobacteria Serratia marcescens and Pseudomonas fluorescens towards root-knot nematode Meloidogyne spp. on strawberry plant grown in naturally infested soil and consequently their probable effects on growth parameters, chemical components and yield of strawberry compared to untreated control. Results of this study confirmed that all used treatments, significantly reduced all related nematode parameters i.e. number of galls/root system; number of egg masses/root system as well as number of juveniles/250 g soil ﴾either after 2 or 4 months﴿ when compared to plants grown in the naturally infested soil only. The highest percentage of reduction in all related nematode parameters were noticed after 4 months. Mulching, S. marcescens, P. fluorescens and tunnels, played an important role in reducing the percentage of nematode parameters. Mulching and S. marcescens reduced the percentage of gall numbers/root system by 77%, followed by tunnels by 75% and P. fluorescens by 70%. S. marcescens reduced the percentage of egg masses/root system by 80% followed by P. fluorescens by 78% and mulching by 76%. Results indicated that the soil treated with mulching, also significantly reduced the percentage of juvenile numbers/250 g soil by 79% followed by S. marcescens by 73%. Results confirmed also that all used treatments significantly increased all related plant growth characters viz. leaves, stems, root fresh and dry weight; plant height, leaves number, root length as well as false stem length. Moreover, these treatments significantly increased the activity of some oxidative enzymes e.g. peroxidase and phenoloxidase as well as some other chemical components i.e. total soluble solids (T.S.S %) and vitamin C (V.C). Results revealed that all previous treatments significantly increased the early and total yield. Our results confirmed that, the rhizobacterium S. marcescens came in the first rank as a promoter of all related plant growth characters, followed by tunnels and mulching treatments. Results confirmed that also the antioxidants, are considered the effective one in enhancing the activities of peroxidase and phenoloxidase as well as T.S.S% and V.C followed by the natural yeast extract

The phytochemical studies on Zingiber officinale rhizomes revealed that it contains traces of flavonoid, carbohydrates, tannins, steroles or terpenoids and it is free from alkaloids.Assay of essential oil of Zingiber officinale rhizome was also carried out. The alcoholic extract of the plant rhizome, was examined against five tumor cell lines, BHK-2, HEPG-2, MCF-7, HCT-2 and HEP-2, using SRB. assay. All cell lines were growth inhibited in a dose dependent manner after exposure to the plant extract. The antitumor activity of the plant extract using E.A.C. method, showed a high activity against mice tumor.

This study aimed to establishing a protocol for producing in-vitro plants of Rosa Damascene. The culture was conducted at the Commission of Biotechnology in Damascus. Four types of explants were cultured on MS medium and some factors affecting culture were examined. The results showed that no viruses were observed, the lateral buds were superior over other explants, then the lateral microcuttings, after that, the apical microcuttings, and, finally, the shoot tips. The highest multiplication rate was observed at the hormonal combinations of (benzyl adenine BA 3mg/I with indole-3-acetic acid IAA 0.1 mg/1), and the highest elongation average were observed at ( IAA 0.1 mg/I with BA 2-6mg/I) or ( indole-3-butyric acid (IBA) 0.1 with (BA) 5-6 mg/I). The transferring was positively effective. The highest rooting percentage was observed when naphthalene acetic acid NAA or IBA were used. (Berlite: peatmoss, 1:1) was the best growing medium for hardening.

The recent study aims to: identify the degree of farmers implementations for the recommendations of the technical rationalization of water use in New valley Governorate. In addition, identifying the independent variables affecting the degree of farmers implementations for the recommendations of the technical rationalization of water use as a dependent variable. In order to accomplish the study objectives, new valley Governorate was selected as a filed study. A systematic random sample of 212 farmers was selected. This sample represents about 5% of the total farmers in three villages in New valley Governorate. A questionnaire was designed and collected using the personal interview (after it was pre tested) during may 2011. Then, the data were tabulated and analyzed by using simple correlation coefficient, stepwise regression. The study finding showed that about 14% of the total sample have low level of implementations for the recommendations of the technical rationalization of water use. Medium category represents about 50%, and high category of implementations for the recommendations of the technical rationalization of water use represents about 36% of the total sample. Results revealed by using stepwise regression showed that three variables affected the degree of implementations for the recommendations of the technical rationalizations of water use, and these variables explained about 51% of the total variance of the implementations for the recommendations of the technical rationalization of water use. Those variables were: farmland holding size, respondent attitude towards the extension activities, finally respondent degree of formal social participation.

. The effects of neem (Azadirachta indica) and willow (Salix babylonica) aqueous extracts on fusarium wilt disease in tomato seedlings were investigated. Four weeks old tomato seedlings were treated with 10% of either neem and willow aqueous extracts and then infected with Fusarium oxysporum after 4 days of treatment. The results showed that the percentage of disease incidence was increased in non treated tomato seedlings in time dependent manner and reached the maximum level (65%) after 6 weeks of infection. Treatments of tomato plants with neem and willow aqueous extracts reduced the percentage of disease incidence to the level of 25.5% and 27.8% after 6 weeks of infection respectively. The results show that infection of tomato seedling with Fusarium oxysporum led to many morphological and biochemical changes including, reducing the growth of tomato shoot and root, increasing the level of lipid peroxidation and marked increase in the activities of antioxidant defensive enzyme I.e. POX, CAT, and SOD. Treatment with neem and willow aqueous extracts significantly exhibited a growth promotion of tomato shoot and root in infected or non infected seedling. Moreover, application of neem and willow aqueous extracts with fusarium, significantly reduce the level of lipid peroxidation and induce high activities of antioxidant defensive enzymes after 3 and 7 days of infection. Electrophoretic pattern of POX demonstrated that Fusarium oxysporum caused up regulation of several POX isoenzymes. It could be concluded that neem and willow aqueous extracts reduced the disease incidence of fusarium wilt in tomato seedlings by increasing the activities of antioxidant defensive enzymes and decreasing the level of lipid peroxidation.

Among 17 fungal isolates isolated from soil of El-hawia, El-hada, El-kaym and Karwa in Taif governorate in Saudi Arabia, two isolates showed high efficacy in producing cellulases enzymes. They were identified to be Altemaria altemata and Aspergillus wentii. Some factors such as carbon and wheat bran as a raw material, nitrogen, pH and incubation temperature were investigated. Results indicated that glucose and cellulose were the most effective as a carbon source while, urea was the best nitrogen source for cellulases production. Initial pH 5.0 and incubation temperatures at 25 or 35°C achieved high cellulases production.

Detergents are an undetectable source of pollution, which hidden in most of our daily activities. Detergents could cause harmful effects before they are completely degraded. It is wise to reduce the amount of detergents that usually used by invention new bio-friendly formula contains efficient enzymes such as protease. Screening studies were carried out for one hundred and fifty bacterial isolates with respect to their ability to produce protease(s), after growing on slaughter house wastes (SHW) isolated from EI-Khorma governorate,Taif, Kingdom of Saudi Arabia (KSA) at 55°C, and pH 9. The most potent thermophilic bacterial isolate concerning of alkaline thermostable protease(s) production was identified as Bacillus licheniformis EGT50. Alkaline thermostable proteases productivity by the most potent bacterial isolate was affected by substrate concentrations (solid substrate), carbon source, nitrogen source, amino acid supplements, incubation temperature, incubation period, and inoculum size. Maximum both enzymes production by B. licheniformis EGT50 was obtained on SHW concentrations, 7.5 %; galactose; diammonium hydrogen phosphate; arginine at 55°C for 72 h. when inoculated by 0.5 ml. The protease production under all optimal conditions was increased many folds from 563.68 to 17825 U/ml (31 fold). The purification fold of B. licheniformis EGT50 alkaline thermostable protease increased to 394.7 after applying Sephadex G200 column chromatography techniques. The enzyme productivity of protease has been determined and the result proved the possibility to use the crude and purified enzymes in biodetergent technology.