Water deficit stress is considered as one of the most important factors affecting soil microbial activities in dry and semi-dry ecosystems, which reduces microbial activity by limiting access to nutrients. To evaluate the effect of water stress (30%, 50%, 70%, and 100% of field capacity), urea chemical fertilizer (100, 200, and 300 kg/ha), and the impact of biological fertilizers (inoculation with mycorrhiza alone, co-inoculation with mycorrhiza and nitrogen-fixing bacteria, co-inoculation with mycorrhiza and azospirillum, and control) on the yield characteristics of Zea mays hybrid Sc706, a field experiment was conducted as a split-split plot in a randomized complete block design with three replications in two cropping seasons (2018-2019 and 2019-2020) on a farm in Firoozabad, Fars province, Iran. Dual and triple interactions of water stress, urea chemical fertilizer, and biological fertilizer showed significant differences in all yield characteristics. In most traits, the application of urea chemical fertilizer (200 and 300 kg/ha) along with biological fertilizers (mycorrhiza-azospirillum and mycorrhiza-nitrogen-fixing bacteria treatments) produced the best results. Overall, it can be concluded that the use of 200 and 300 kg/ha of urea fertilizer along with a combination of biological fertilizers, mycorrhiza-azospirillum and mycorrhiza-nitrogen-fixing bacteria, can be suitable options for improving the yield and morpho-physiological characteristics of corn under regional conditions.