Pearl Millet (Pennisetum glaucum L.) is one of the most important crops in arid and semi-arid regions that are cultivated for grain and forage. Due to cross pollination and high heterosis, the development of hybrid varieties is the most important breeding method of pearl millet. In order to study the preliminary yield comparison of hybrid cultivars and investigate the possibility of replacing open-pollinated cultivars with hybrid cultivars, 11 promising pearl millet hybrid cultivars with a check variety (Mehran cultivar) were planted in the randomized complete block design with three replications in Karaj, Isfahan and Zabul. The days to 50% flowering, plant height, stem diameter, leaves number, panicle length, panicle thickness, tiller number, fresh fodder yield, dry matter yield, 1000 seed weight, and seed yield were evaluated. The results of combined variance analysis and mean comparisons showed that there is considerable genetic diversity for the studied traits. The results showed that the cultivars H294, H444, H504, H604, H824, and H794 had the highest grain yield compared to the total mean of the experiment. In addition, five cultivars H543, H333, H353, H563, and H604 were above of total average in terms of fodder production and had good potential to be used in the field as commercial cultivars with the aim of fodder and seed production.