Cimetidine is a known reproductive toxicant due to its adverse effects on testicular function. Melatonin is an antioxidant that has a role in mitigating any disorders that could affect spermatogenesis. Therefore, this study evaluated the ameliorative potential of melatonin on the adverse reproductive effects of cimetidine in piroxicam-induced gastric ulcerated male rats. Thirty rats were randomly divided into six groups (n=5), including normal control (distilled water), ulcerated and non-treated, ulcerated and treated with cimetidine (200mg/kg day), ulcerated and treated with cimetidine and melatonin (10mg/kg), treated with cimetidine and melatonin without ulceration, and ulcerated rats treated with only melatonin. All treatments were done orally per day for 14 days. On the 15th day, rats were sacrificed, and blood samples were collected for hormone and antioxidant assays. Then, the sperm parameters were analyzed according to standard procedures. Co-administration of melatonin to piroxicam-ulcerated rats treated with cimetidine showed a significant increase (p < 0.05) in sperm count and epididymal glutathione peroxidase compared to piroxicam-ulcerated rats treated with only cimetidine. Melatonin increases the serum level of Testosterone, FSH, and LH.  Melatonin ameliorates the adverse reproductive effects of cimetidine through its antioxidant properties. Therefore, co-administration of melatonin with cimetidine in male ulcer patients is recommended.

The current study aimed to investigate the potential antimicrobial and antioxidant activities of Eremurus spectabilis (E. spectabilis) in three extraction techniques. Three methods were selected to extract the aqueous, alcoholic, and hydroalcoholic extracts of E. spectabilis. The extraction yield was obtained from 10 g of E. spectabilis powder. The carbohydrate test was performed using the phenol sulfuric acid method. The Kjeldahl method was used in two replicates based on the AOAC 2550 standard to determine the protein content. The concentration of phenolic compounds was measured by the Folin-Ciocalteu assay. Based on the results, E. spectabilis had 70.33% w/w carbohydrates and 7.1% w/w proteins. The extraction percentages for the aqueous, alcoholic, and hydroalcoholic extracts of E. spectabilis were 50%, 10%, and 25%, respectively. The results showed that the aqueous extraction method was the most efficient. The total phenol amount for E. spectabilis aqueous extract was 150.04 mg/g. The antioxidant property of the E. spectabilis aqueous extract was determined to be 50.71%  None of the concentrations of the aqueous extract did not have antimicrobial properties against Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa. These findings demonstrates the need for further studies on other pathogens and using different concentrations.

The European badger (Meles meles) is a powerful animal native to Europe and parts of West Asia. The skull imparts the phylogenetic morphology to the skulls of animal species and functions as a safeguard for vital organs, including the brain and eyes. The aim of our study was to improve the current data by providing a comprehensive investigation of the morphology and dimensions of the cranium and mandible of badgers. This information enhances comprehension of radiological and surgical attributes. We conducted anatomical studies and measured the morphometric features. We received the skulls from naturally occurring carcasses. We employed unpaired t-test for statistical analysis. This study demonstrated that the anatomical features of the skull exhibit no discernible differences between badgers and other wild carnivores, such as tiger and wolf. The presence of a twin jugular foramen in the skull of badgers distinguishes them as a distinct characteristic not found in any other carnivorous animals. Moreover, radiographical studies showed two distinct sinuses and a cavity in the skull of badger which is different from dogs. Males and females differ significantly in some morphometric traits, which is entirely consistent with the behavioral and nutritional traits of the animal. Modern imaging techniques, such as CT scans, are necessary for more thorough studies on the skulls of wild carnivores.

Type 1 diabetes mellitus (T1DM) occurs due to the decrease in insulin secretion following the destruction of pancreatic beta cells. This disease is increasing worldwide, especially among children under the age of 5 years, which is usually associated with irreversible complications such as hepatopathy and nephropathy. The present study aimed to investigate the antidiabetic effect of the heat-killed Actinomycetales species, including Gordonia bronchialis (Gb), and Tsukamurella inchonensis (Ti) in streptozotocin-diabetic rats by oral administration. This experiment was performed in six groups, including healthy control, diabetic control, low-dose Gb (G1), high-dose Gb (G2), low-dose-Ti (T1), and high-dose Ti (T2). Subsequently; the levels of ALT, AST, total protein, albumin, BUN, creatinine, CRP, IL-1β, and IL-2 were measured in the serum samples in the 14th and 21st days. Besides, histopathological lesions were studied in the liver and kidney. Our findings showed that Gb and Ti could alter the examined serum parameters, particularly in the T2 groups. Also, histological examination revealed a remarkable attenuation in the pathological lesions such as focal necrosis, vascular congestion, and hemorrhage in the liver and kidney of the treated rats by Gb and Ti. Here, it is concluded that oral administration of the heat-killed Actinomycetales species, particularly with a high dose of Ti, could beneficially improve the progression of T1DM and its various complications, which can be used to treat T1DM in the future.

This study aimed to investigate the protective effects of pomegranate peel (PoP) extract on the gill, liver, and kidney tissues of common carp exposed to cadmium (Cd). For this purpose, 150 common carp weighing 65 ± 0.85 g were randomly divided into five groups with three triplicates for each group (30 fish per group). The control group received a standard diet without cadmium chloride (CdCl2), the Cd group was exposed to 0.5 mg/L CdCl2, and the extract groups received PoP extract with concentrations of 1%, 2%, and 4% (percentage of food weight) along with 0.5 mg/L CdCl2 in the water. After four weeks, tissue samples were collected from the gill, liver, and kidney and stained with hematoxylin and eosin for histopathological examination. In the gills of the Cd group, lesions included congestion, hemorrhage, clubbing or fusion of the secondary lamellae, and telangiectasia of the lamellae. The liver tissue of the Cd group exhibited severe degeneration and necrosis of hepatocytes, while hemorrhage, congestion, cellular degeneration or necrosis, and hyaline cast were visible in the kidney tissue of this group. The severity of the mentioned lesions was significantly reduced in the PoP extract groups, particularly at concentrations of 2% and 4% (p < 0.05). Based on the results, it can be concluded that PoP extract has significant protective effects on the gill, liver, and kidney tissues of common carp exposed to CdCl2.

keeping rabbits as pets and their use in laboratory research increased the need for studying heart diseases and treatments in rabbits. Pimobendan is one of the most common medications used in cardiac diseases and is anecdotal in rabbits. The first step toward the approval of pimobendan in rabbits is assessing the potential for beneficial effects on cardiac function through echocardiographic functional parameters.This study aimed to determine the effects of pimobendan on echocardiographic parameters. Eleven rabbits were included in this study. Echocardiographic examinations were performed before and after pimobendan administration for each rabbit. The LV morphological and functional parameters were compared between study time points. Pimobendan resulted in changes in several echocardiographic variables in the rabbits, including FS and an increase in EF, SV, LVPWs, and LA end-systolic (p = 0.0001, p = 0.0001, p = 0.0284, p = 0.0272, and p = 0.0007, respectively). Moreover, LVIDs and end-systolic volume decreased (p = 0.0343 and p = 0.038). The changes in some parameters were not significant, such as LVIDd, LVPWd, end-diastolic volume, LA max,  Mitral annulus diameter, and LA/Ao. FS, which indicates an increase in the power of heart contraction and consequently an improvement in heart function, increased in this study after pimobendan administration. Therefore, it can be concluded that pimobendan improves cardiac functions. Further studies are required to investigate whether pimobendan has similar effects in rabbits with cardiac diseases.

Considering the role of skull bones in preserving vital organs, paying close attention to the shape and size of the skull is of great importance when various conditions, such as head trauma, are suspected. However, in order to confirm the suspicion radiologically, examiners need to have detailed information on the normal skull characteristics of each breed. This study aimed to evaluate the radiological and anatomical features of the skull in adult Husky dogs. The current descriptive cross-sectional study examined eight adult Husky dogs (four males and four females) that died due to different conditions, excluding those of the head. After the preparation of the skulls, radiographs were obtained on different views. In addition, the bones were examined in terms of morphological characteristics. Morphometric indices were also measured and the results were recorded. Based on the results of this study, the skull of Husky dogs consists of 11 cranial bones (Ossa cranii), and 21 facial bones (Ossa faciei). Three parameters, including the height and the length of tympanic bullae as well as the orbital index, were greater in females compared to males. However, the differences were not significant. Other parameters were greater in male dogs. The whole skull length and maximum width of the skull showed significant differences between the two genders (p ≤ 0.05). The precise standards obtained in the current study can be used in interpreting the results to determine whether the presenting characteristics are abnormal or breed-dependent.

Poultry meat production worldwide has continued to expand over the last two decades. In this regard, hygienic meat inspection and monitoring of diseases at slaughter lines have been recognized as essential for assessing flocks' status. This study aimed to determine the condemnation rate of slaughtered poultry and calculate the economic losses due to condemnations in the Namin industrial slaughterhouse, Ardabil Province of Iran. The data were collected by a veterinarian inspector in the slaughterhouse. The number of poultry slaughtered, their weight, the number and weight of condemned carcasses, and the reasons for condemnation were recorded.  In this study, 3,488,916 poultry were slaughtered, and 42,310 carcasses (1.202 %) were condemned, weighing 66,385 kg. The highest percentage of condemned poultry was observed in autumn (1.61%), and the lowest in spring (0.93%). The direct financial loss incurred due to condemnations was estimated to be as high as 153,067 USD. Septicemia and dead on arrival (DOA) were the most common reasons for the rejection of carcasses, accounting for 47.85% and 0.580 of the total condemnations and total slaughtered poultry, respectively. The highest frequency percentage of the condemnation due to diseases occurred during autumn.In contrast, summer had the highest condemnation rate in association with DOA. The current survey showed that diseases caused the most condemnations compared to other causes. Therefore, improving disease control programs on flocks and increasing the welfare of birds before slaughter is recommended.

Borna disease virus (BDV) is a neurotropic, enveloped and ribonucleic acid (RNA) virus. BDV induces persistent neurologic disease in a wide host range included several vertebrate species as well as human. The BDV genome encodes 6 proteins but p24 protein was identified at higher rates than other proteins at BDV-infected tissues. In this study, BDV-p24 protein was constructed and subcloned into expression plasmid pET22. Confirmation of recombinant protein expression was performed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis and western blotting. P24 protein was injected into rabbits with the aim of polyclonal antibody production and immunization. Compared to other diagnostic methods, ELISA is a fast method with cost effective and high sensitivity as well as lower probability of contamination. ELISA method was performed to evaluate the infection in laboratory rabbits and retrospective infection was examined in 50 rabbits. The obtained results in this study indicated that the ELISA method based on p24 protein has a high potential to detect BDV infection.

Foodborne diseases are a significant global public health concern due to their high prevalence, mortality, and economic losses. The aim of this study was to conduct a systematic review of identified foodborne pathogens and outbreaks in Iran over the past 23 years to provide an overview of the risk assessment and prevention approaches in the country. Using appropriate keywords and searching major databases, such as ScienceDirect, Scopus, PubMed, Google Scholar, and the Iranian Scientific Knowledge Database, we initially identified 4,740 articles. Finally, 328 articles were selected for evaluation. Among these articles, publications on Salmonella, Staphylococcus aureus, and Listeria were the most numerous. Poultry meat was found to be the main source of major foodborne pathogens in Iran, including Campylobacter (46.21%), Listeria monocytogenes (38.45), Salmonella (24.83%), and Yersinia enterocolitica (16.81%). Given the high prevalence of foodborne bacteria in Iranian foods, it is crucial to implement effective control measures to reduce the risk and burden of foodborne diseases. In particular, poultry meat, which poses a high risk for the occurrence of foodborne diseases in Iran, should be subjected to further risk assessment and control measures throughout the food chain.