Уточнить поиск
Результаты 1-6 из 6
Protective effects of intranasal vaccination with plasmid encoding pseudorabies virus glycoprotein B in mice
1999
Takada, A. (Hokkaido Univ., Sapporo (Japan)) | Okazaki, K. | Kida, H.
Intranasal administration of plasmid DNA encoding glycoprotein B of pseudorabies virus into mice induced both serum and secretory antibody responses. These mice resisted intranasal challenge with lethal dose of the virus, but did not intraperitoneal challenge. On the other hand, intramuscular injection of the plasmid induced less secretory and higher serum antibody responses than those of intranasally vaccinated mice. None of them was protected from virus challenge. The present results suggest that administration of plasmid DNA encoding glycoprotein B by respiratory mucosal route generates local secretory antibodies which serve to protect animals from pseudorabies virus infection
Показать больше [+] Меньше [-]Characterization of DNA fragment from Chlamydia psittaci avian strain which shows high homology with HypB gene of Chlamydia
1991
Sato, C. (Hokkaido Univ., Sapporo (Japan). Faculty of Veterinary Medicine) | Katumata, A. | Takashima, I. | Hashimoto, N.
A rapid and simple transcriptional sequencing method for GC-rich DNA regions
2006
Izawa, M.(Nippon Genetech Co. Ltd., Toyama (Japan)) | Kitamura, N. | Odake, N. | Maki, F. | Kanehira, K. | Nemoto, H. | Yamaguchi, M. | Yamashita, A. | Sasaki, N. | Hattori, M. | Kanayama, S. | Yoneda, Y.
In genome sequencing project, we encounter the DNA regions that often contain stable secondary structure with high GC content. These regions are difficult to not only amplify by PCR for template preparations, but also deter mine the DNA sequences using standard Cycle sequencing (CS) method. Transcriptional sequencing (TS) is a unique DNA sequencing method using RNA polymerase, and is based on the principles of the chain-termination method, which is a powerful method to analyze GC-rich sequences. In this study, we examined the multiple displacement amplification (MDA) to overcome low efficiency of PCR amplification in GC-rich regions and subjected to TS reaction. Combination of MDA and TS (MDA-TS) was extremely successful with GC content ranging from 65 % to 85%, which are difficult to analyze with PCR and CS. We also report plasmid vector, pTS1, which has the stronger T7 and T3 promoters than those of conventional vectors, and the sequence that decreases transcriptional efficiency was removed from its multiple cloning sites. pTS1 resulted in the improved sequencing accuracy and reduced reaction time up to 5 min. These results showed that MDA-TS is a rapid and accurate method for the analysis of GC-rich templates.
Показать больше [+] Меньше [-]Possible conservation units of the sun bear (Helarctos malayanus) in Sarawak [Malaysia] based on variation of mtDNA control region
2006
Onuma, M.(Hokkaido Univ., Sapporo (Japan)) | Suzuki, M. | Ohtaishi, N.
The mitochondrial DNA control region of the sun bear (Helarctos malayanus) was sequenced using 21 DNA samples collected from confiscated sun bears to identify conservation units, such as evolutionarily significant units and management units, in Sarawak, Borneo Island. A total of 10 haplotypes were observed, indicating the presence of at least two lineages in the sun bear population in Sarawak. Presumably, these two lineages could represent evolutionarily significant units. However, the geographical distributions of the two lineages remained unknown due to the lack of information regarding the exact capture locations of the confiscated sun bears. It is essential to elucidate the geographical distributions of these lineages in order to create a proper conservation plan for the sun bears in Sarawak. Therefore, further studies examining the haplotype distributions using DNA samples from known localities are essential.
Показать больше [+] Меньше [-]Detection of viral genome in non-neural tissues of cattle experimentally infected with bovine herpesvirus 1
1996
Mweene, A.S. (Hokkaido Univ., Sapporo (Japan)) | Okazaki, K. | Kida, H.
Confirmed nucleotide sequence of fanF of Escherichia coli K99 fimbriae
1993
Abe, N. (National Inst. of Health, Tokyo (Japan)) | Moriishi, K. | Saito, M. | Naiki, M.