Bovine pulmonary artery cells in cell culture were exposed to lipopolysaccharide (LPS) purified from Pasteurella haemolytica serotype A1. This resulted in severe membrane damage, which caused a time- and dose-dependent release of lactate dehydrogenase that was first detected 4 hours after exposure and reached a maximal mean release of 67% after 24 hours of exposure to 1 microgram of LPS/ml. Mean release of 51chromium followed by a similar pattern and reached a maximum of 61% following 24 hours of exposure to 10 micrograms of LPS/ml. Morphologically, endothelial cells responded to LPS by marked cell membrane retraction, the formation of numerous cytoplasmic blebs, and ruffling of the cell membrane. Subsequently, the cells became round and detached. Cell detachment reached a mean of 95% following 8 hours of exposure to 1 microgram of LPS/ml. These studies demonstrated that P haemolytica LPS is capable of causing direct damage to bovine pulmonary arterial endothelial cells, which may be important in the pathogenesis of bovine pneumonic pasteurellosis.

The vasculature of 22 small colons from dead adult ponies was perfused with latex or barium sulphate solution. The vascular anatomy was studied by use of dissection and alkali digestion of the latex specimens and microangiography of the barium sulphate-perfused specimens. The small colon is supplied by the caudal mesentric artery. The left colic artery arises from the caudal mesenteric artery, which then becomes the cranial rectal artery. Branches from the left colic and cranial rectal arteries form anastomosing arcades that become narrower distally along the length of the small colon. From these arcades arise terminal arteries, which enter the small colon wall and give rise to a subserosal, an intermuscular, and a large submucosal plexus, with frequent anastomoses between them. The venous drainage closely parallels the arterial supply, except near to its origin from the portal vein, when the left colic vein and caudal mesentric vein are separate from the corresponding arteries.

This study was carried out to investigate the origin, insertion, direction of muscle fibers and structure of the ear muscles of the Korean native goat. The description was based on the dissection of fifteen Korean native goats with embalming fluid. The ear muscles of the Korean native goat were composed of the Musculus zygomaticoauricularis, M. scutuloauricularis superficialis, M. scutuloauricularis profundus, M. frontoscutularis, M. interscutularis, M. parietoauricularis, M. cervicoscutularis, M. cervicoauricularis superficialis, M. cervicoauricularis medius, M. cervicoauricularis profundus, M. auricularis profundus posterior and M. parotidoauricularis. The M. frontoscutularis clearly seperated into temporal and frontal parts in 6 cases. The M. scutuloauricularis profundus clearly separated into major and minor parts. The M. zygomaticoauricularis blended with the M. parotidoauricularis near its insertion, but not with the M. scutuloauricularis.

This study was carried out to investigate the branch and distribution of Nervus facialis of the Korean native goat. The observation was made by dissection of embalmed cadavers of ten Korean native goats. N. facialis arose from the ventrolateral surface of the medulla oblongata. In the facial canal, N. facialis gave off N. petrosus major, N. stapedius and Chorda tympani. N. petrosus major arose from Ganglion geniculi, passed through the pterygoid canal and terminated in Ganglion pterygopalatium. Chorda tympani joined N. lingualis at the lateral surface of the internal pterygoid muscle.

The anatomical structure of pelvic limb of thirty-one adult Korean native goats (Body weight : 14-17kg) was observed after skeletal preparation, and the osteometry was performed in each bone. The pelvic limb of the Korean native goat was composed of the hip bone, femur, patella, tibia, fibula, tarsal bones, metatarsal bone, phalanges and sesamoid bones. The hip bone consisted of the ilium, ischium and pubis which fused each other. The gluteal surface of the ilium was directed dorsolaterally. The tuber sacrale and tuber coxae were formed at the dorsal apex and ventrolateral part of the iliac wing, respectively. The lesser ischiatic notch was deeper than the greater one. The ischiatic tubercles were triangular and consisted of the dorsal, lateral and caudal ischiatic tubercles. The left and right hip bone indexes were 67.08, 66.20, the acetabular indexes were 93.78 and 92.10 and the obturator formen indexes were 53.84 and 54.77, respectively. In femur, both of the greater and lesser trochanter were well developed but the third trochanter was not observed. The left and right femur indexes were 26.55 and 26.14, head indexes were 81.66 and 81.49 and the trochlear-epicondyle indexes were 42.47 and 41.63, respectively. The patella was observed as an isosceles triangle with base lying proximal and the cranial surface was more convex. The tibial shaft was sigmoid (S-) shaped and the popliteal notch was deep. There was a large nutrient formen at the cranial aspect of the cranial intercondylar area. The tibial indexes were 22.09 (left) and 21.10 (right). The proximal extremity of the fibula was fused with the lateral condyle of the tibial but the distal one was observed independently as the malleolar bone.

This study was undertaken to obtain basic data of the sternal development in Korean native cattle from the earliest sternal formation to the ossification using histological and histochemical methods. Thirty three sterna were collected from a series of embryos and fetuses ranging from 11 to 225mm (estimated age 37-120 days) in crown rump length. The bilateral sternal bars were observed in the 2nd group (CRL 21-30mm) of Korean cattle embryos. Those bars initiated to be fused in the 3rd group (CRL 31-40mm) and completed in the 7th group (CRL 71-80mm). The ossification centers were detected in the 8th group (CRL 81-90mm) also bilateral ossification centers were found in the same group. The typical epiphyseal plates, endochondral bone and calcium deposit were found in the 9th group (CRL 91-100mm). Osteocytes, osteoblasts, osteoclasts and myeloid cells appeared in ossification centers in the 10th group (more than CRL 101mm). The alcianophility responded markedly in the 9th group that was decreased and showed slightly positive reaction in territorial matrix of the 10th group. Marked positive reaction to PAS was observed in bony trabeculae in the 10th group. The positive reaction to calcium deposit by trichrome stain was observed initially in the hypertrophied zone of epiphyseal plate in the 9th group and was conspicuous in the calcified zone of epiphyseal plate in the 10th group. The 1st positive reaction to the von Kossa stain was observed in the 9th group.

The blood supply to the proximal sesamoid bone of the equine forelimb was examined in 18 cadaver limbs from adult horses, using x-ray computed tomography and a tissue-clearing (Spalteholz) technique. Results of the study indicated that the proximal sesamoid bones were supplied by multiple branches of the medial and lateral palmar digital arteries, which entered the proximal half of the bones on their nonarticular, abaxial surface. After entering the bone, the vessels traverse dorsally, axially, and distally, arborizing into several smaller branches that appear to supply the entire bone. The major branches of these vessels reside in bony canals, the orientation and distribution of which parallel the radiographic lucencies seen in horses with sesamoiditis and correspond to the configuration of apical fracture patterns.

Laparoscopy was performed on 6 horses (2 mares, 2 geldings, 2 stallions) to determine the normal laparoscopic anatomy of the equine abdomen. After withholding feed for 36 hours, horses were examined from the left and right paralumbar fossae, and the visceral anatomic structures were recorded by videotape and photography. One mare developed emphysema located subcutaneously at the primary laparoscopic portal; otherwise, there were no complications. The anatomic structures of diagnostic importance that were observed in the left half of the abdomen were the hepatic duct; left lateral and quadrate lobes of the liver; stomach; spleen; left kidney with the associated nephrosplenic ligament; segments of jejunum, descending colon, and ascending colon; left side of the male and female reproductive tracts; urinary bladder; vaginal ring; and mesorchium. Important structures observed in the right side of the abdomen were portions of the common hepatic duct; left lateral, quadrate, and right lobes of the liver; caudate process of the liver; stomach; duodenum; right dorsal colon, epiploic foramen; omental bursa; right kidney; base of the cecum; segments of jejunum, descending colon, and ascending colon; urinary bladder; right half of the male and female reproductive tracts; and rectum.