Two hundred samples (cow’s and goat’s milk75 for each,their farm soil 25and 25water samples )from different origin have been cultured on Ashdown selective agar which gave a higher percentage (36.5%)for B. pseudomallei isolation , 64% was observed in farm soil followed by water samples 48% than cow’s milk33.33% and finally the goat’s milk 26.66%. According to L-arabinose biotyping , 39.72% of the B.pseudomallei isolates were positive for arabinose (Arb+) and 60.3% of isolates were negative for arabinose (Arb-).The virulence testing revealed that most bacterial isolates yield haemolysin, protease, lipase and lecithenase in a percentage of 97.3% , 82.2%, 89.04% and 84.9% respectively.The Arb- biotypes were more virulent than Arb+ biotypes with significant differences (P< 0.05).All bacterial isolates were characterized by their ability to form capsule(100%),.This study confirmed the presence of casein proteolytic and necrotizing enzymes in the suspension of B.pseudomallei .Proteins concentration in this suspension was 0.00248 mg/ml .