Thirty-seven local isolates of Actinobacillus suis-like organisms from diseased and clinically normal horses and 1 Ilama were compared with reference strains of A suis, A lignieresii, A equuli, A capsulatus, A hominis, A (Pasteurella) ureae, and equine A suis-like organisms (ASLO) previously described in literature. Comparison was by cultural characteristics, carbohydrate fermentation, enzyme profiles, and whole-cell protein polyacrylamide gel electrophoresis. Carbohydrate fermentation, determined by API-CH gallery, divided 36 equine ASLO isolates into 6 API-CH biotypes. The Ilama isolate was an additional distinct biotype. The biochemical comparisons between A suis and ASLO did not reveal remarkable and consistent differences. Enzyme analysis revealed 5 API-ZYM biotypes, one of which included the same strains as one of the API-CH biotypes and consisted in both instances of 4 esculin-negative ASLO cultures and the reference strain of A lignieresii. We conclude that the 4 strains were hemolytic variants of A lignieresii. Protein electrophoresis disclosed 15 banding patterns, 10 of which represented equine ASLO strains. The reference strains of A suis shared the pattern predominant among equine ASLO. Four of the remaining reference strains of Actinobacillus species each had a unique profile, whereas the type strain of A capsulatus and the Ilama isolate had similar profiles. The groupings of cultures resulting from the different testing methods had little relation to each other and to the anatomic source of the strains except the strains comprising API-CH biotype III, which originated in the equine respiratory tract, and the A lignieressi cluster.

To investigate the epidemiological trait of intestinal diseases of animals caused by thermophilic Campylobacter spp., isolation of etiological agent was carried out. Isolated Campylobacter spp. were biotyped, serotyped and the susceptibility of the isolates to antimicrobial agents were examined. Isolation rates of Campylobacter spp. from 649 fecal materials of 208 cattle, 300 pigs and 141 chickens were 25.5 %, 23.7 % and 38.3 %, respectively. The majority of the 130 isolates of C jejuni was classified as biotype I (50.6 %) and biotype II (34.6 %). Most of the 46 isolates of C coli were biotype I (71.7 %). Isolated C jejuni strains showed 14 different serotype, and serotype 4, 26, 36 were most frequent. Isolated C coli strains showed 5 different serotype and serotype 31 and 21 were relatively common. Isolated Campylobacter spp. were highly susceptible to nalidixic acid, amikacin, gentamycin, colistin and chlorampehnicol

Serological study and isolation of Brucellae from dairy cattle were carried out in Kyungbook area of Korea during the period from 1984 to 1985. Biotyping of the isolates was done. Of the 11168 cattle in 2479 herds examined, 27 cattle (0.24%) in the 17 herds (o.69%) were serologically positive for brucellosis. Brucella abortus was recovered from 20 (74.1%) of the 27 infected cattle, and all the strains of the isolates belonged to biotype 1. Of the tissues cultured for brucellae, the supramammary lymph nodes had the highest rate (55.6%) of recovery of brucella organisms