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Structural implications of the EL(K/Q)(L/C)LD(A/G)DD sequence in the C-terminal cytoplasmic tail for proper targeting of anion exchanger 1 to the plasma membrane
2009
Adachi, H., Hokkaido Univ., Sapporo (Japan) | Ito, D. | Kurooka, T. | Otsuka, Y. | Arashiki, N. | Sato, K. | Inaba, M.
While the C-terminal cytoplasmic tail of anion exchanger 1 (AE1, band 3) has been reported to possess important physiological roles, including one for proper membrane trafficking, its precise characteristics remain unclear. To clarify the overall structural consequences of the conserved sequence EL(K/Q)(L/C)LD(A/G)DD, containing the core binding sequence LDADD for carbonic anhydrase II, in the C-terminal region, we analyzed the membrane expression and turnover of bovine AE1 with a series of truncation and substitution mutations in HEK293 cells. Immunofluorescence microscopy and cell-surface biotinylation demonstrated that truncation mutants missing 18 C-terminal residues targeted the plasma membrane, but the one lacking the conserved region, by truncation of 28 amino acid residues, was retained inside the cells. Substitutions of Ala for Glusup(901), Leusup(902), Leusup(905), and Aspsup(906) in the sequence E901L(K/Q)(L/C)LDADD909 of bovine AE1 or those in the corresponding murine sequence also caused intracellular retention, though these mutants had half-lives comparable to that for wild-type AE1. These data demonstrate that the conserved amino acid residues Glusup(1), Leusup(2), Leusup(5), and Aspsup(6) in the EL(K/Q)(L/C)LD(A/G)DD region have essential structural consequences in stable expression of AE1 at the plasma membrane regardless of the ability in binding to carbonic anhydrase II of this region.
Показать больше [+] Меньше [-]Plasma Trace Mineral Concentration of Kutuchi Camels.
2009
Shukla, M.K. | Siddiquee, G.M. | Latifand, A. | Parekar, S.S.
The present study reports the concentration of Copper, Zinc, Iron and Manganese in the blood plasma of male kutchi camels during their breeding season. The respective concentrations of the plasma trace minerals were 112.94 ±O.44, 105.65±2.08, 117.65±1.72 and 160.29±0.75 µ/dl.
Показать больше [+] Меньше [-]Обменные нарушения у телят при респираторном синдроме
2009
Kovzov, V.V., Vitebsk State Academy of Veterinary Medicine (Belarus)
Determination of degree and relations between derangements of metabolic processes in calves with respiratory syndrome in the milking period was realized in the conditions of the Republic of Belarus. Research results showed that the calves with the respiratory syndrome had expressed metabolic processes disturbances, which were marked in the analyzed biochemical blood indexes (total protein; albumins; urea; creatinine; glucose; cholesterin; triglycerids; bilirubin; ALP; AAT; SGPT; calcium; phosphorous; magnesium; iron; manganese; cobalt; cuprum; zinc). In calves with respiratory syndrome there was noted the increased content of crude protein in blood serum (71,42 +/- 2,57 g/l P less than 0,05), low urea level (3,08 +/- mmol/l). There was the increased level of creatine (574,11 +/- 65,26 mkmol/l; P less than 0,01) and bilirubin (8,11 +/- 1,15 mkmol/l; P less than 0,05). Glucose concentration in blood of diseased calves was at below standard level (3,49 +/- 0,69 mmol/l). Glucose indexes varied and were changing during a day; and depended on muscular activity, intervals between feeding and hormonal regulation. Diseased calves had high cholesterol level in blood serum (4,07 +/- 0,34 mmol/l; P less than 0,05), which was stated in case of liver disease and hypothyroidism. Phosphor level was higher than the standard indexes of healthy calves (2,85 +/- 0,03 and 2,05 +/- 0,05, respectively). Content of magnesium, cobalt and zinc in blood of the diseased calves was lower than in healthy ones. Calves with respiratory pathology had protein, fat, hydro-carbonic and mineral metabolic processes
Показать больше [+] Меньше [-]Уровень показателей белкового обмена у жеребят первой половины подсосного периода
2009
Makovskij, E.G. | Motuzsko, N.S., Vitebsk State Academy of Veterinary Medicine (Belarus)
Study of peculiar features of protein metabolism of healthy colts in the first three months of life was realized in the conditions of the Republic of Belarus. Research results showed that there was a tendency of increasing of hemoglobin concentration up to 150,270+/- 4,73 g/l, number of total protein of blood serum – up to 60,33 +/- 1,33 g/l, and the decreasing of uric acid up to 97,33 +/- 10,72 micromole /l. Quantity of beta-1 and alpha globulin fractions of serum protein fluctuated a little – from 10,78 +/- 3,69% up to 8,44 +/- 1,24% and from 25,12 +/- 4,84% up to 22,06 +/- 4,93%, respectively. The most essential changes of protein metabolism were stated at two month old colts, which had urea concentration of 5,25 +/- 0,23 micromole/l, alanine aminotransferase activity – 66,92 +/- 3,16 units/l, creatine content increased up to 77,37 +/- 5,83 micromole /l; and there was the lowering of aspartate transaminase level up t159,5 +/- 8,1units/l. There was stated the lowering of albumin quantity up to 32,07 +/- 2,19%, and increasing of alpha-1, alpha2- and beta2- globulin fractions up to 7,52 +/- 1,23%, 14,98 +/- 1,41%, 11,82 +/- 1,04%, respectively. At the age of three months the quantity of urea and creatinine in blood serum of colts remained at the same level, alanine aminotransferase activity decreased on 21,56%, and aspartate transaminase increased up to 204,33 +/- 10,37 units/l. At this period the quantity of albumin fractions was 44,56 +/- 6,59%., concentration of alpha-1, alpha2- and beta2- globulins decreased on 10,64%, 30,04% and 34,52%, respectively
Показать больше [+] Меньше [-]Ген эритропоэтинового рецептора (EPOR) - новый ген-маркер многоплодия свиноматок
2009
Dojlidov, V.A. | Kaspirovich, D.A., National Academy of Sciences. Scientific and Practical Center of Animal Breeding (Belarus) | Loban, N.A., National Academy of Sciences. Scientific and Practical Center of Animal Breeding (Belarus) | Bannikova, A.D., All Russian Research Inst. of Animal Breeding, Dubrovitsy (Russian Federation)
Determination of influence of polymorphism of gene of erythropoetin receptor (EPOR) on productive qualities of sows and reproductive characters of boars of Belarusian meaty breed was realized in the conditions of the Republic of Belarus. Research results proved the possibility of EPOR gene application as a genetic marker which determined productive abilities of swine of Belarusian selection breed. Frequency of undesirable allel EPOR**c of EPOR gene in the analyzed swine stock balanced within the frames of wide range: from 44% for Belarusian meaty breed sows up to 78% for Belarusian Kripnaya Belaya breed sows. Analysis of influence of genotype with EPOR gene on multiple fetation of sows of Belarusian meaty breed showed that sows with the preferred genotype (EPOR**tt) statistically reliable (P less than 0,01) exceeded sows with undesirable genotype (EPOR**cc) in accordance with number of alive birth piglets on 1,7 heads. Among sows of Belarusian Kripnaya Belaya breed with genotype EPOR**ct there were stated multiple fetation indexes exceeding the sows with genotype EPOR**tt on 0,5 heads. Desirable EPOR**tt genotype irrespective of sow age rendered the positive influence on their multiple fetation. It was recommended to realize genetic testing on determination og polymorphism of gene of erythropoetin receptor among the animals of Belarusian meaty and Belarusian Kripnaya Belaya breeds. Selection of parental pairs of the selection nucleus was recommended to realize taking into consideration genotypes, giving preference to animals with EPOR**t allel
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