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Devastating endosulfan poisoning in Korean native cattle
2013
Lee, B., Animal and Plant Quarantine Agency, Anyang, Republic of Korea | Lee, H.K., Animal and Plant Quarantine Agency, Anyang, Republic of Korea | Lee, K.H., Animal and Plant Quarantine Agency, Anyang, Republic of Korea | Yoon, S.S., Animal and Plant Quarantine Agency, Anyang, Republic of Korea | Kim, M., Animal and Plant Quarantine Agency, Anyang, Republic of Korea | Park, J.W., Animal and Plant Quarantine Agency, Anyang, Republic of Korea | Jeong, S.H., Gyeonggido Veterinary Service, Suwon, Republic of Korea | Lee, M.H., Animal and Plant Quarantine Agency, Anyang, Republic of Korea | Bae, Y.C., Animal and Plant Quarantine Agency, Anyang, Republic of Korea
Here, we report the poisoning case of 10 cows. Several distinct clinical signs such as convulsion, excessive salivation, circling, lateral recumbency, and death were observed. Necropsy and histopathological examination did not reveal any significant abnormal findings. Moreover, no bacteria or viruses were detected in tissue, blood, and feeding food. However, endosulfan was detected from the stomach contents and microbials. Our results strongly suggest that death of cows may be closely associated with endosulfan poisoning.
Показать больше [+] Меньше [-]The virulence of Brucella abortus isolated from cattle in Korea
2011
Lim, J.J., Gyeongsang National University, Jinju, Republic of Korea | Kim, J.H., Northern Branch, Gyeongbuk Veterinary Service Laboratory, Andong, Republic of Korea | Kim, D.H., Gyeongsang National University, Jinju, Republic of Korea | Lee, J.J., Gyeongsang National University, Jinju, Republic of Korea | Kim, D.G., Gyeongsang National University, Jinju, Republic of Korea | Jun, M.H., Chungnam National University, Daejeon, Republic of Korea | Kim, S.H., Chungnam National University, Daejeon, Republic of Korea | Chang, H.H., Gyeongsang National University, Jinju, Republic of Korea | Lee, H.J., Gyeongsang National University, Jinju, Republic of Korea | Min, W.G., Gyeongsang National University, Jinju, Republic of Korea | Kim, S., Gyeongsang National University, Jinju, Republic of Korea
In this study, we isolated 12 of Brucella (B.) spp. from cattle, which have been positive in Rose Bangal test and tube agglutination test in Gyeongbuk province in 2009. According to AMOS PCR analysis, isolated 12 strains were identified as B. abortus. Murine derived macrophage, RAW 264.7 cells, were infected with isolated 12 strains or reference strain (B. abortus 544), and bacterial internalization were characterized. According to these results, we divided the isolated strains into the following three groups: class Ⅰ, lower internalization than that of B. abortus 544; class Ⅱ, similar internalization to that of that of B. abortus 544; class Ⅲ, higher internalization than that of B. abortus 544 within RAW 264.7 cells. Furthermore, intracellular growth, bacterial adherent assay, LAMP-1 colocalization, virulence in mice and surface protein pattern were characterized. From these results, representative strains of class Ⅲ showed lower LAMP-1 colocalization, higher adherent efficiency, higher virulence in mice than those of B. abortus 544, and showed different pattern of surface proteins. These results suggest that B. abortus field strains, isolated from cattle in Korea, possess various virulence properties and higher internalization ability of field strain may have an important role for its virulence expression.
Показать больше [+] Меньше [-]Biological characterization of Brucella spp. isolated from cattle in Gyeongbuk, Korea
2010
Kim, J.H., Northern Branch, Gyeongbuk Veterinary Service Laboratory, Andong, Republic of Korea | Lim, J.J., Gyeongsang National University, Jinju, Republic of Korea | Kim, D.H., Gyeongsang National University, Jinju, Republic of Korea | Lee, J.J., Gyeongsang National University, Jinju, Republic of Korea | Kim, D.G., Gyeongsang National University, Jinju, Republic of Korea | Jun, M.H., Chungnam National University, Daejeon, Republic of Korea | Kim, S.H., Chungnam National University, Daejeon, Republic of Korea | Chang, H.H., Gyeongsang National University, Jinju, Republic of Korea | Lee, H.J., Gyeongsang National University, Jinju, Republic of Korea | Min, W.G., Gyeongsang National University, Jinju, Republic of Korea | Kim, S., Gyeongsang National University, Jinju, Republic of Korea
Members of the genus Brucella are facultative intracellular bacteria and cause brucellosis, a chronic disease in humans and abortion in animals. In this study, we tested sera for brucellosis of 15 Hanwoo farms in the western part of Gyeong-buk province, resulting 5 farms were brucellosis positive in 2008. We collected blood from 277 heads in the brucellosis positive 5 farms, and performed serological diagnosis, brucella positive cattle which had shown higher than 200 antibody titer in tube agglutination test were slaughtered, supramammary lymph nodes were collected, and Brucella spp. wild type isolation and identification were performed. From these results, 15 of Brucella spp. wild type strains were isolated and all strains were identified as B. abortus biotype 1 by biological and molecular analysis. In the antimicrobial susceptibility test, all 15 strains had a similar susceptibility and resistance pattern. This study may be useful for bacteriological and epidemiological understanding of cattle brucellosis in Korea.
Показать больше [+] Меньше [-]Study of plasma beta-carotene concentration in dairy cows
1999
Nam, H.M. | Moon, J.S. | Joo, Y.S. (National Veterinary Research & Quarantine Service, Anyang (Korea Republic).) | Oh, T.H. | Park, Y.H. | Han, H.R. (Seoul National University, Suwon (Korea Republic). College of Veterinary Medicine)
This study was carried out to determine the effects of beta-carotene on the control of mastitis in dairy cows during the dry period. The relationship between the levels of plasma beta-carotene and the status of udder health in Holstein dairy cows were investigated. Blood samples were collected from 117 cows to compare the levels of plasma beta-carotene in lactating cows. The levels of plasma beta-carotene were 1.82 micro gram/ml in healthy cows(n=65) and 1.12 micro gram/ml in mastitic cows(n=52), respectively(p0.01). In the experiment to compare the level of plasma beta-caroten in the cows at different stages of lactation, the plasma beta-carotene levels were 1.73 micro gram/ml in lactating cows(n
Показать больше [+] Меньше [-]Evaluation of micronucleus frequency in cytokinesis-blocked lymphocytes of cattle in the vicinity of Uljin nuclear power station
Kim, S.R.;Kim, S.H.(Chonnam National University, Gwangju, Republic of Korea)E-mail:shokim@chonnam.ac.kr | Kang, C.M.(Korea Institute of Radiological and Medical Science, Seoul, Republic of Korea)
Cytogenetic and hematological analysis was performed in peripheral blood of cattle in the vicinity of Uljin nuclear power station and control area. The frequency of micronuclei(MN) in peripheral blood lymphocytes from cattle was used as a biomarker of radiobiological effects resulting from exposure to environmental radiation. An estimated dose of radiation was calculated by a best fitting linear-quadratic model based on the radiation-induced MN formation from the bovine lymphocytes exposed in vitro to radiation over the range from 0 Gy to 4 Gy.
Показать больше [+] Меньше [-]Seroprevalence of Q-fever in Korean native cattle
2014
Kim, J.Y., Animal and Plant Quarantine Agency, Anyang, Republic of Korea | Sung, S.R., Animal and Plant Quarantine Agency, Anyang, Republic of Korea | Pyun, J.I., Animal and Plant Quarantine Agency, Anyang, Republic of Korea | Her, M., Animal and Plant Quarantine Agency, Anyang, Republic of Korea | Kang, S.I., Animal and Plant Quarantine Agency, Anyang, Republic of Korea | Lee, H.K., Animal and Plant Quarantine Agency, Anyang, Republic of Korea | Jung, S.C., Animal and Plant Quarantine Agency, Anyang, Republic of Korea
Q-fever is a vector-borne (Coxiella [C.] burnetii) zoonotic disease that is an increasing public health concern. To date, some research about Q-fever prevalence in dairy herds and human patients has been reported in Korea, but information about Korean native cattle is scarce. To measure the prevalence rates of C. burnetii in Korean native cattle, a total of 1,095 bovine serum samples collected during 2010~2013 were analyzed with an enzyme-linked immunosorbent assay. Sixty-eight heads of cattle were diagnosed as positive and while 19 heads were suspected (positive rate = 6.2%). Interestingly, Jeju province had a seropositivity rate six times greater than that of other provinces (18.9% vs. 3.2%). High seroprevalence might be caused by wide distribution of ticks in Jeju province compared to other regions. Based on these data, extensive monitoring of C. burnetii infection in cattle, tick distribution, and climate changes is required.
Показать больше [+] Меньше [-]Epitheliogenesis imperfecta in a bovine fetus of Korean native cattle
2014
Rhyoo, M.Y., Animal and Plant Quarantine Agency, Anyang, Republic of Korea | Jung, J.Y., Animal and Plant Quarantine Agency, Anyang, Republic of Korea | Her, J.W., Animal and Plant Quarantine Agency, Anyang, Republic of Korea | Lee, M.H., Animal and Plant Quarantine Agency, Anyang, Republic of Korea | Ku, K.N., Institute for Gyeonggi-do Veterinary Service, Bucheon, Republic of Korea | Choi, K.R., Institute for Gyeonggi-do Veterinary Service, Bucheon, Republic of Korea | Yoon, S.S., Animal and Plant Quarantine Agency, Anyang, Republic of Korea
In this study, we describe a case of epitheliogenesis imperfecta (EI) observed in the fetus of Korean native cattle. The fetus had multifocal areas of skin defect, especially on the distal portions of the four limbs, and the affected areas were bright-red and glistening. Histopathologically, these areas were characterized by complete absence of squamous epithelium, infiltration of inflammatory cells into the dermis, atrophy of hair follicles, sebaceous and sweat glands. To the best of our knowledge, this is the first report of epitheliogenesis imperfecta in Korean native cattle.
Показать больше [+] Меньше [-]Comparative studies of histopathologic pulmonary Lesion of cattle and deer tuberculosis by Mycobacterium bovis
2008
Jean, Y.H. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea), E-mail: jean@nvrqs.go.kr | Roh, I.S. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea) | Lee, K.H. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea) | Lee, K.W. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea) | Cho, Y.S. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea) | Joo, Y.S. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea)
Comparative studies of histopathologic lesions from 23 purified protein derivative (PPD) positive cattle, 21 slaughter cattle found with tuberculosis, and 11 tuberculosis-positive elk (Cervus elaphus) were performed. PPD positive cattle did not show specific histopathologic lesions in all 23 heads that were no visible lesion reactor. Slaughter cattle found with tuberculosis revealed microscopically classical granulomatous lesion (tubercle) with central caseous necrosis surrounded by mantle of epithelioid cells and Langhan's giant cells capsuled by connective tissue in lung. Elk was noted with some different lesion patterns with classical granulomatous lesion and suppurative abscesses that was composed of fibrin, degenerated cells without having connective tissue. In addition, many Langhan's giant cells infiltration in alveoli at peripheral lesion were observed in some cases of classical granulomatous lesion and suppurative abscesses.
Показать больше [+] Меньше [-]Pathological study on abdominal fat necrosis of adult cattle sampled from slaughterhouse in Korea
2005
Lee, J.C. (Seojeong College, Yangju, Republic of Korea) | Kim, J.S. (Chonnam National University, Gwangju, Republic of Korea) | Suh, G.H. (National Livestock Research Institute, RDA, Cheonan, Republic of Korea) | Lee, C.G. (Seojeong College, Yangju, Republic of Korea) | Kim, S.K. (Seojeong College, Yangju, Republic of Korea) | Cho, K.O. (Seojeong College, Yangju, Republic of Korea) | Kang, M.I. (Seojeong College, Yangju, Republic of Korea) | Jeong, C. (Seojeong College, Yangju, Republic of Korea) | Park, S.H. (Seojeong College, Yangju, Republic of Korea) | Lee, C.Y. (Seojeong College, Yangju, Republic of Korea), E-mail: cylee@chonnam.ac.kr
An abattoir study on the abdominal fat necrosis in adult cattle was performed pathologically. Grossly, masses of fat necrosis were leekgreen in colour, lobulated on the cut surface, and saponificated in the texture. These necrotic adipose tissues infiltrated usually into neighboring parenchymal organs including intestines and pancreas, leading to fibrosis or atrophy of them. Histopathologically, necrotic fat cells contained acidophilic, opaque, amorphous substance or basophilic fibrillar or granular minerals in their cytoplasms. The lesions of fat necrosis were divided by fibroconnective tissue. With increase of the severity, necrotic fat cells fused each other and then formed fat cysts.
Показать больше [+] Меньше [-]The ability of in vitro cultured bovine oviduct epityelial cells in binding and maintaining motility of bull sperm
1999
Roh, S.H. (Hankyong National University, Ansung (Korea Republic). Department of Animal Life Resources) | Lee, B.C. | Hwang, W.S. (Seoul National University, Suwon (Korea Republic). College of Veterinary Medicine)
The aim of these experiments was to investigate the effects of bovine oviduct epithelial cells(OEC) derived from different segments to bind sperm binding and maintain their motility in vitro. In experiment 1, the number of sperm attached to OEC derived from isthmus or ampulla, the motility of unattached sperm during co-culture and fertilizing ability were assessed. In experiment 2, heparin treated sperm 9hsp) or no treated sperm (nsp) were used to evaluate OEC binding ability ofcapacitated sperm. In experiment 1, regardless ofthier origin, approximately 65% of the sperm were attached to OEC within 2h. From 6h of co-culture, the numbers of unattached sperm on ampullary OEC were significantly higher than those on isthmic OEC (p0.005). From 12h of co-culture, the motility of unattached sperm on isthmic OEC were significantly higher than those on ampullary OEC(p0.05). The cleavage rate of oocytes inseminated on OEC derived from isthmic segment was also significantly higher than those from ampullary segment (p0.01). In experiment 2, the numbers of unattached hsp on OEC were significantly higher than those of controls(p0.01), between 2~24h examination. From 12h of co-culture, the motility of unattached nsp were sighificantly greater than those of hsp(p0.01). These results show that bovine OEC derived from the isthmus play more important role(s) for sperm binding, maintaining motility and fertilization in vitro than those from the ampulla, and heparin induced capacitation may change sperm binding ability on OEC in vitro.
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