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Bovine papillomavirus detection from bovine teats using immunohistochemistry and electronmicroscopy
2005
Bae, Y.C. (National Veterinary Research and Quarantine Service, MAF, Anyang, Republic of Korea), E-mail: baeyc@nvrqs.go.kr | Lee, C.S. (Chungbuk Livestock and Veterinary Research Institute, Cheongwon, Republic of Korea) | Kang, M.I. (Chonnam National University, Gwangju, Republic of Korea) | Yoon, S.S. (National Veterinary Research and Quarantine Service, MAF, Anyang, Republic of Korea) | Park, J.W. (National Veterinary Research and Quarantine Service, MAF, Anyang, Republic of Korea) | Jean, Y.H. (National Veterinary Research and Quarantine Service, MAF, Anyang, Republic of Korea)
We examined the teats of slaughtered cattles to investigate the prevalence of papilloma and to detect bovine papilloma virus (BPV) from those samples. Among 880 heads, 432 (49.0%) was Holstein and 448 (51.0%) was Korean native cattle. Grossly, out of 432 heads (Holstein), 263 (60.8%) had papilloma lesions on teats. However, out of 448 heads (Korean native cattle), only 33 (7.4%) had papilloma lesions on teats. Immunohistochemically, BPV antigen was detected in 35 heads (22.9%) of 153 (Holstein) which had papilloma lesions. But, in Korean native cattle, BPV antigen was detected in 1 head (3.8%) of 26.
Показать больше [+] Меньше [-]Detection and phylogenetic analysis of bovine papillomavirus in cutaneous warts in cattle in Tamaulipas, Mexico
2016
Rojas-Anaya, Edith | Cantu-Covarrubias, Antonio | Álvarez, José Francisco Morales | Loza-Rubio, Elizabeth
Papillomas occur more frequently in cattle than other domestic animals. The causal agent of bovine papillomatosis is a virus that belongs to the family Papillomaviridae. In Tamaulipas, Mexico, the virus is considered a serious problem and has impeded the export of cattle to the United States, resulting in serious economic losses. Owing to the lack of information regarding the subtypes of papillomaviruses that infect cattle in Mexico, the aim of this study was to determine the subtypes in Tamaulipas. Fifty-two warts were analyzed with the use of polymerase chain reaction (PCR) involving primers that amplify the E7 gene of bovine papillomavirus (BPV). The PCR products were sequenced to differentiate the BPV-1 and BPV-2 subtypes. The sequencing quality was determined with the use of MEGA 6.0 software. Comparison of the Tamaulipas sequences with those of known BPV types by means of the MUSCLE algorithm showed that 53% of the former were BPV-1 and 47% were BPV-2. The distribution of the 2 subtypes in the cattle was homogeneous. This study demonstrated the presence of BPV-1 and BPV-2 in cattle from Tamaulipas and constitutes the first molecular characterization of papillomas in Mexico.
Показать больше [+] Меньше [-]Lack of effect of recombinant bovine interferon alpha(I)1 in the treatment of experimentally-induced bovine warts
1993
Lassauzet, M.L. | Salamin, P.A.
