Brucellosis is a major constraint to livestock production that still enzootic in livestock in many developing countries including Egypt. This study was conducted with the general objective of establishing the bacteriological status of bovine brucellosis in 15 governorates in Egypt during 2020-2021 to determine the circulating Brucella species on bacteriological and molecular basis. Clinical samples collected included milk or udder secretions, vaginal discharges, fetal membranes and stomach contents of aborted fetuses from dairy cows with history of brucellosis. In addition, lymph nodes (retropharyngeal, prescapular, prefemoral, internal iliac and supramammary) from carcasses of serologically positive animals were obtained from different localities for isolation and identification of Brucella organisms. A total of 136 Brucella isolates were recovered from cattle in different governorates, Egypt. These include, 107 isolates of Brucella melitensis biovar 3 identified on bacteriological and molecular basis from Aswan, Beheira, Beni Suef, Dakahlia, Damietta, Fayoum, Gharbia, Giza, Ismailia, Kafr El-Sheikh, Luxor, Monufia, Port Said, Qalyubia and Sharqia governorates. On the other hand, 29 Brucella abortus biovar 1 isolates were recovered from cattle from Beni Suef, Dakahlia, Damietta, Kafr El-Sheikh, Monufia, Port Said and Sharqia governorates. Molecular identification using primer sequences targeting IS711 gene confirmed Brucella on genus level. Multiplex PCR has amplified four fragments of 450bp, 587 bp, 1071 bp, and1682 bp characteristic for B. melitensis biovar 3, and three fragments of 450bp, 587 bp, and 1682 bp for B. abortus biovar 1. The identification of Brucella spp. in different farm animals of 15 Egyptian governorates highlights the dynamics and role of cattle in dissemination of Brucella infection all over the country. The obtained results indicate that the actual Brucellosis status during the years 2020 and 2021 refers to that B. melitensis biovar 3 and B. abortus biovar 1 are the prevalent types circulating in different Egyptian governorates.

Objective: Brucellosis is an endemic zoonotic infection in Saudi Arabia, including Jazan Province, and epidemiological reports about this disease are limited. In this study, we aimed to investigate knowledge, attitudes, and practices regarding brucellosis among the general population of Jazan Province, southwestern Saudi Arabia. Materials and Methods: This is a cross-sectional study conducted using a pretested questionnaire that assessed the level of knowledge, attitudes, and practices toward Brucellosis. The calculated sample size was 384, and 1,055 participants were included representing various genders, ages, and levels of education. Data were collected between March and April 2022 and analyzed using descriptive, chi-square, and t-test analyses. Results: Only 50% of the included participants have heard about Brucellosis. Among those who had heard about Brucellosis, 70% had a good knowledge about Brucellosis and that was significantly associated with male gender, being healthcare workers (HCWs), and having a higher level of education. Participants who directly involved in animal care represented 9%, and attitudes and practices regarding brucellosis were varied from average to unsatisfactory. This variation was significantly affected by the level of knowledge. Conclusion: About 50% of the general population of Jazan Province had never heard about Brucellosis, in a region found prevalent for this disease. Good knowledge was recorded in 70% of those who were aware of Brucellosis, which was significantly associated with the male sex, job as being HCW, and having a higher level of education. These results necessitate public awareness campaign activities to improve knowledge and practices, especially among women, the younger generation, and individuals with limited educational backgrounds. This action could reduce the burden of the disease. [J Adv Vet Anim Res 2022; 9(4.000): 761-769]