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Presence and characterization of Campylobacter jejuni in organically raised chickens in Quebec
2011
Thibodeau, Alexandre | Fravalo, Philippe | Laurent-Lewandowski, Sylvette | Guévremont, Evelyne | Quessy, Sylvain | Letellier, Ann
The objective of this study was to estimate the presence of the important foodborne pathogen Campylobacter jejuni in organically raised chickens in the province of Quebec. The recovered isolates were further characterized for their antimicrobial resistance profile, autoagglutination property and chemotaxis. Antimicrobial resistance was evaluated using agar dilution for: tetracycline, erythromycin, chloramphenicol, ciprofloxacin, gentamicin, nalidixic acid, clindamycin, ampicillin, azithromycin, bacitracin, and ceftiofur. Autoagglutination was measured by monitoring optical density changes in a bacterial suspension after 3 h of incubation at room temperature. Chemotaxis was evaluated after a contact time of 3 h between isolates and mucin, using a quantitative protocol. A total of 10 lots of chickens was sampled in August and September 2009; half of them were positive for the presence of C. jejuni. Antimicrobial resistance was found only for tetracycline (44%), erythromycin (6%), azithromycin (6%) and clindamycin (2%). Variation was observed in the minimum inhibitory concentrations (MICs) for ceftiofur and bacitracin, for which C. jejuni possess intrinsic resistance. Autoagglutination and chemotaxis varied among isolates and lot-level differences in these were observed. Autoagglutination and chemotaxis levels appeared as independent isolate properties. Further monitoring and characterization of isolates originating from organic chickens is of interest since this type of production might represent another source of exposure of consumers to a variety of the foodborne pathogen C. jejuni.
Показать больше [+] Меньше [-]Effects of diet formulations containing proteins from different sources on intestinal colonization by Campylobacter jejuni in broiler chickens
2003
Udayamputhoor, Roy S. | Hariharan, Harry | Van Lunen, Ted A. | Lewis, P Jeffrey | Heaney, S. (Susan) | Price, Lawrence | Woodward, David
The objective of this study was to compare the effects of 3 diet formulations containing different protein sources (animal, plant, and a combination of animal and plant) on the colonization of Campylobacter jejuni in the gastrointestinal tract of broiler chickens. A freshly isolated strain of C. jejuni (biotype IV, serotype HS O:21, O:29, HL untypable) from a broiler chicken was used to infect 3-day-old chicks that had been free of C. jejuni; 0.5 mL of an inoculum containing 108 colony-forming units was administered orally. Shedding of the organism was studied, and C. jejuni in the ceca, jejuni, and crop were enumerated by quantitative culture. The isolates recovered from the birds during the study period of 35 d were characterized and confirmed as C. jejuni by the use of standard methods and underwent biotyping, serotyping, antimicrobial susceptibility testing by disk diffusion and the E-test, and flagellin gene typing. A cyclical pattern of shedding of C. jejuni was observed in all the birds. Colonization was highest in the ceca. The ceca of birds receiving plant-protein-based feed had significantly less colonization then the ceca of birds receiving the other types of feed, whereas the differences in colonization of the jejuni and crops were not significant. Characterization by biotyping, serotyping, and flagellin gene typing showed that 95% of the recovered isolates were identical to the strain used for infecting the chicks. However, with the Lior-HL typing scheme, 74% of the recovered isolates were HL untypable. Antimicrobial resistance testing did not reveal significant differences between the infecting strain and the recovered isolates among the different feed groups.
Показать больше [+] Меньше [-]Comparative study of colonizing and noncolonizing Campylobacter jejuni
1991
Meinersmann, R.J. | Rigsby, W.E. | Stern, N.J. | Kelley, L.C. | Hill, J.E. | Doyle, M.P.
Campylobacter jejuni A74/O and A74/C are congenic strains. An oral dose of 10(5) organisms of strain A74/C colonizes chicken intestines. Strain A74/O, from which A74/C is derived, does not colonize the chicken intestines with an oral dose of 10(5) organisms. In this study, the congenic bacteria were compared to identify possible colonization mechanisms. Differences were not observed in plasmid content or by HindIII, Pst I, Acc I, HincII, Ava I, Ava II, Xba I, and BamHI restriction enzyme digestion of total DNA. Transmission electron microscopy of negatively stained samples revealed no differences between the strains. Sections of cecal tissue from nonfed day-of-hatch chicks were cultured with each strain for 2 hours and then examined by light and electron microscopy. Both strains caused necrosis of villus epithelial cells. Immunofluorescent or silver staining revealed strain A74/C located deep in numerous epithelial crypts, but strain A74/O only was present in one sample mixed with sloughed necrotic cells. Similarly, organisms were detected by transmission electron microscopy deep in crypts in tissues cultured with A74/C, but not A74/O. Cells of A74/C detected in crypts did not appear to associate with epithelial cells. The strains did not differ in chemotactic behavior to mucin or fucose.
Показать больше [+] Меньше [-]Fluoroquinolone resistance and phylogenetic analysis of broiler Campylobacter jejuni isolates in Indonesia
2023
Sheila M. Yanestria | Mustofa H. Effendi | Wiwiek Tyasningsih | Aswin R. Khairullah | Shendy C. Kurniawan | Ikechukwu B. Moses | Rosmita Ikaratri | Muhammad E.E. Samodra | Fidi N.A.E.P. Dameanti | Otto S.M. Silaen | Mariyono Mariyono | Abdullah Hasib
Consumption of poultry contaminated with Campylobacter jejuni is the main source of sporadic campylobacteriosis in humans, while fluoroquinolone resistance is increasing in Campylobacter jejuni isolated from poultry. The aim of this study was to detect the incidence of fluoroquinolone resistance and analyze phylogenetics by sequencing gyrase subunit A from broiler Campylobacter jejuni isolates. The contents of 200 chicken intestines were taken from chicken farms in 4 sub-districts (Sukorejo, Pandaan, Kejayan, and Grati) in Pasuruan Regency, Indonesia. The Kirby-Bauer Diffusion Test method is used to detect fluoroquinolone resistance phenotypically. Polymerase chain reaction is used to detect fluoroquinolone resistance genotypically through detection of the gyrA gene. A phylogenetic tree based on gyrA genes was created using MEGA12. The results showed that 31 Campylobacter jejuni isolates had high resistance to nalidixid acid (100%), enrofloxacin (96.7%), and ciprofloxacin (93.6%). All Campylobacter jejuni isolates (100%) were fluoroquinolone resistant phenotypically and had the gyrA gene genotypically. Phylogenic analysis showed that the Campylobacter jejuni gyrA gene sequence isolated from broilers from different sub-districts were highly related. Sequence results from broilers with gyrA gene sequences from humans appear to be in the same cluster, indicating that zoonotic transmission can occur. The discovery of a high percentage of fluoroquinolone resistance genes, where fluoroquinolone is the first line drug for the treatment of diarrhea in humans, should certainly be an important issue related to human health.
Показать больше [+] Меньше [-]Experimental evaluation of tulathromycin as a treatment for Campylobacter jejuni abortion in pregnant ewes
2020
Yeager, Michael J. | Wu, Zuowei | Plummer, Paul J. | Sahin, Orhan | Ocal, Melda Meral | Beyi, Ashenafi F. | Changyun, Xu | Zhang, Qijing | Griffith, Ronald W.
OBJECTIVE To evaluate the efficacy of tulathromycin for prevention of abortion in pregnant ewes when administered within 24 hours after experimental inoculation with Campylobacter jejuni. ANIMALS 20 pregnant ewes between 72 and 92 days of gestation. PROCEDURES All ewes were inoculated with a field strain of C jejuni (8.5 × 108 to 10.6 × 108 CFUs, IV). Eighteen hours later, ewes received either tulathromycin (1.1 mL/45 kg [2.4 mg/kg], SC; n = 10) or sterile saline (0.9% NaCl) solution (1.1 mL/45 kg, SC; sham; 10). Ewes were euthanized immediately after observation of vaginal bleeding, abortion, or completion of a 21-day observation period. Necropsy was performed on all ewes, and tissue specimens were obtained for bacterial culture and histologic examination. RESULTS 1 sham-treated ewe and 1 tulathromycin-treated ewe developed signs of severe endotoxemia and were euthanized within 24 hours after C jejuni inoculation. Seven sham-treated and 2 tulathromycin-treated ewes developed vaginal bleeding or aborted and were euthanized between 4 and 21 days after C jejuni inoculation. The proportion of tulathromycin-treated ewes that developed vaginal bleeding or aborted during the 21 days after C jejuni inoculation (2/9) was significantly less than that for the sham-treated ewes (7/9). CONCLUSIONS AND CLINICAL RELEVANCE Results suggested that administration of tulathromycin to pregnant ewes following exposure to C jejuni was effective in decreasing the number of C jejuni–induced abortions. Because of concerns regarding the development of macrolide resistance among Campylobacter strains, prophylactic use of tulathromycin in sheep is not recommended.
Показать больше [+] Меньше [-]Isolation of some pathogen bacteria from the milk collection tanks
2000
Duzgun, S.(General Directorate of Agricultural Research, Ankara (Turkey)) Inal, U | Turk, N.(Bornova Veterinary Control and Research Inst., Izmir (Turkey))