Abortions in domestic ruminants cause significant economic losses to farmers. Determining the cause of an abortion is important for control efforts, but it can be challenging. All available diagnostic methods in the bacteriology laboratory should be employed in every case due to the many limiting factors (autolysis, lack of history, range of samples) that complicate the investigation process. The purpose of this study was to determine whether the recovery of diagnostically significant isolates from domestic ruminant abortion cases could be increased through the use of a combination of the existing aerobic culture and Brucella selective method with methods that are commonly recommended in the literature reporting abortion investigations. These methods are examination of wet preparations and impression smears stained by the modified Ziehl–Neelsen method, anaerobic, microaerophilic, Leptospira, Mycoplasma and fungal culture. Samples of placenta and aborted foetuses from 135 routine clinical abortion cases of cattle (n = 88), sheep (n = 25) and goats (n = 22) were analysed by the new combination of methods. In 46 cases, bacteria were identified as aetiological agents and in one case a fungus. Isolation of Brucella species increased to 7.4% over two years compared with the previous 10 years (7.3%), as well as Campylobacter jejuni (n = 2) and Rhizopus species (n = 1). Salmonella species (5.9%) and Trueperella pyogenes (4.4%) were also isolated more often. In conclusion, the approach was effective in removing test selection bias in the bacteriology laboratory. The importance of performing an in-depth study on the products of abortion by means of an extensive, combination of conventional culture methods was emphasised by increased isolation of Brucella abortus and isolation of C. jejuni. The combination of methods that yielded the most clinically relevant isolates was aerobic, microaerophilic, Brucella and fungal cultures.

Objective: The objective of this study was to isolate and identify Pasteurella spp. associated with pneumonic lungs showing respiratory signs of goats in Ethiopia.Materials and methods: A total of 2400 goats that were slaughtered at the Hashim&#146;s Ethiopian Livestock and Meat Export abattoir, Ethiopia were randomly selected for this cross-sectional study during the period of October 2013 to July 2014. Detail ante-mortem, and post-mortem (PM) lesions were inspected, and the suspected samples were collected aseptically from the lungs. Among 2400 goats, 31(1.29%) goats were not slaughtered because these goats showed severe clinical signs. Thus, 2369 goats were slaughtered finally. The collected samples were subjected for isolation and identification of bacterial species following conventional methods such as culture and biochemical examinations. Results: Out of 2400 goats examined, 960(40%) animals showed different abnormal respiratory signs. Based on PM findings, 16.21% (n=384/2369) lungs were found as pneumonic, of which 78.38% (n=301/384) were found to be associated with Pasteurella organism. The overall prevalence of Pasteurella organism (Mannheimia haemolytica and Pasteurella multocida) was 12.71% (n=301/2369). In this study, youngers and goats with medium body condition score (BCS) had greater probability (P<0.05) to be infected by the bacteria though there was no difference in exposure to the organism among goats from Arsi, Bale and Hararghe. On the other hand, out of 301 positive cases, 274(91.03%) were caused by M. haemolytica, and 27(8.97%) were caused by P. multocida isolates. Conclusion: Pasteurella organism especially M. hemolytica is one of the most common causes of pneumonic pasteurellosis in caprine at the study area. So, chemoprophylaxis needs to be given to small ruminants prior to transportation or other stress conditions. [J Adv Vet Anim Res 2017; 4(2.000): 147-154]

Objective: A retrospective survey was designed to determine the prevalence and factors involved in surgical cases at the Veterinary Teaching Hospital (VTH), Bangladesh Agricultural University from June 2014 to June 2017.Materials and materials: In total, 1042 surgical cases of food animal (large ruminants: n=564, and small ruminants: n=493) and 26 non-food animal (mono-gastric animal) were recorded from patient register book and case recording card. Data were analyzed by Epi Info TM software and frequencies were calculated for different variables using Statistical Package for Social Science (SPSS) software.Result: In large ruminant, hernia (16.13%) ranked top (90% umbilical and 10% lateral) followed by fracture (14.89%), abscess (14.54%), umbilical myasis (10.46%), atresia ani (5.85%) and naval ill (4.07%). Among the reported cattle, 87.41% were crossbred and 12.59% were indigenous. Calf, heifer and adult cattle were 55.32, 10.29 and 34.39% respectively. In small ruminants, castration (32.94%; n=138) ranked top followed by myasis (10.55%) naval ill (10.31%), abscess (7.44%), dystocia (6.24%) and urolithiasis (5.49%). Based on surgical classification in large and small ruminants, 37.56 and 42.50% were reported for general surgery, whereas 28.71 and 7.15% for congenital, 11.18 and 13.12% for gynecological and 2.65 and 37.23 % for andrological problems, respectively. Male and female ratio was 1:1.31 and 2:1 respectively in large and small ruminants, respectively.Conclusion: This study emphasizes the factors related to successful surgical cases management at VTH. The results may help in controlling surgical related cases in Mymensingh region of Bangladesh. [J Adv Vet Anim Res 2018; 5(1.000): 81-87]

Abortions in domestic ruminants cause significant economic losses to farmers. Determining the cause of an abortion is important for control efforts, but it can be challenging. All available diagnostic methods in the bacteriology laboratory should be employed in every case due to the many limiting factors (autolysis, lack of history, range of samples) that complicate the investigation process. The purpose of this study was to determine whether the recovery of diagnostically significant isolates from domestic ruminant abortion cases could be increased through the use of a combination of the existing aerobic culture and Brucella selective method with methods that are commonly recommended in the literature reporting abortion investigations. These methods are examination of wet preparations and impression smears stained by the modified Ziehl-Neelsen method, anaerobic, microaerophilic, Leptospira, Mycoplasma and fungal culture. Samples of placenta and aborted foetuses from 135 routine clinical abortion cases of cattle (n = 88), sheep (n = 25) and goats (n = 22) were analysed by the new combination of methods. In 46 cases, bacteria were identified as aetiological agents and in one case a fungus. Isolation of Brucella species increased to 7.4% over two years compared with the previous 10 years (7.3%), as well as Campylobacter jejuni (n = 2) and Rhizopus species (n = 1). Salmonella species (5.9%) and Trueperella pyogenes (4.4%) were also isolated more often. In conclusion, the approach was effective in removing test selection bias in the bacteriology laboratory. The importance of performing an in-depth study on the products of abortion by means of an extensive, combination of conventional culture methods was emphasised by increased isolation of Brucella abortus and isolation of C. jejuni. The combination of methods that yielded the most clinically relevant isolates was aerobic, microaerophilic, Brucella and fungal cultures.

Incidence of seroconversion to caprine arthritis-encephalitis virus (CAEV) was determined for 1,194 goats on 11 dairies, using 2 repeated annual herd tests for CAEV. Current life table methods were used to compare age-specific incidence of seroconversion for pasteurized milk-raised and unpasteurized milk-raised goats. Logistic regression models were used to determine the risk factors associated with CAEV seroconversion, and to estimate odds ratios for seroconversion for various factor levels. Goats raised by unpasteurized milk-feeding methods were 2.5 to 6.7 times more likely to seroconvert than were goats raised by pasteurized milk-feeding methods, depending on the method of comparison. Similarly, 61.6 to 85.0% of seroconversions in yearling goats possibly were attributable to unpasteurized milk feeding. Among yearling goats, CAEV seroconversion was associated with feeding method, breed, and source of goat (herd of origin) when the effect of dairy was considered. In addition to the 6.7 times greater risk of seroconversion for unpasteurized milk-raised goats, yearling goats of the Saanen and Toggenburg breeds were 2.2 and 3.3 times, respectively, more likely to seroconvert than were Alpine yearling goats. Yearling goats purchased from another source were less likely to seroconvert than were yearlings raised on the dairy where they were studied. Among goats > 1 year old, age was associated with risk of seroconversion. Goats that were 3 years old or were > 4 years old were 1.7 and 3.2 times, respectively, more likely to seroconvert than were 2-year-old goats, when adjusted for effect of dairy. The effects of dairy were significant (P less than or equal to 0.001) in yearling and older goats.

A prospective observational cohort study of 361 dairy goat kids was conducted to compare 2 methods of controlling caprine arthritis-encephalitis virus infection under commercial dairy conditions. To compare effectiveness of feeding kids pasteurized milk vs serologic testing and segregation in addition to pasteurized milk feeding, goats were monitored up to the age of 30 months by use of monthly agar gel immunodiffusion testing. Survival analysis methods were used to determine whether age at seroconversion differed between the 2 groups. Significantly lower rates of seroconversion were observed in the segregated group (P < 0.001), compared with the nonsegregated group. Of 193 goats in the pasteurized milk-only group, 146 (75.6%) seroconverted within the 30-month study period, whereas infection was detected in 39 (23.2%) of 168 goats in the test/segregated group. Nonsegregated goats were 3.37 times more likely to seroconvert by 24 months of age, and 70.3% of seroconversions by 24 months of age could be attributed to nonsegregation. For age-specific intervals beyond 180 days of age, 70 to 100% of seroconversions could be attributed to lack of segregation. Cohort life tables for age at seroconversion were reported for each group. Type of colostrum fed, sex, and weaning group (season) were not significantly associated with age at seroconversion. Saanen goats had lower age-specific risk of seroconversion in the nonsegregated group alone and overall. Non-Saanen goats were 1.5 times more likely to seroconvert than were Saanen goats, when adjusted for a possible confounding effect of weaning group. Results indicate that pasteurized milk feeding and routine test and segregation would be a substantially more effective means of control of the disease in dairy goat herds than would pasteurized milk feeding alone.

Log-linear methodology was used to examine relations among caprine arthritis-encephalitis virus (CAEV) seroreactivity and host/management factors in a cross-sectional study of 2,826 goats on 13 California dairies. The CAEV serologic status was associated with age and feeding method (pasteurized/unpasteurized milk), but not with breed. Data from a prevalence survey of 321 goats from 2 additional dairies demonstrated very good fit of the selected log-linear model (P = 1.00), indicating that the model was very appropriate to describe the relations. Odds of seropositivity and odds ratios were generated by use of a logit model derived from the log-linear model. Goats raised by the unpasteurized feeding method were estimated to have been 3.3 times more likely to be CAEV-seropositive than goats fed by the pasteurized method, when adjusted for the effects of age. Goats aged 2, 3, 4, and 5 or greater years were estimated to have been 1.7, 2.6, 4.5, and 5.7 times, respectively, more likely to be CAEV-seropositive than were yearling goats when ratios were adjusted for pasteurization status. Breed, gender, and herd of origin were not associated with CAEV seroreactivity when the effects of other factors were considered. Estimated odds of CAEV seroreactivity and the associated odds ratios for combinations of factor levels are reported. In this study, the magnitude and direction of the associations among CAEV serologic status, age, and pasteurized feeding methods were demonstrated.

Objetivando-se avaliar o efeito da refrigeração sobre o exame hemogasométrico, foram utilizados 14 caprinos machos, hígidos, mestiços, com cerca de quatro meses de idade e peso variando entre 30 e 45 kg. As amostras de sangue destinadas ao exame hemogasométrico foram coletadas em duplicata, utilizando-se agulhas descartáveis acopladas a seringas plásticas contendo cerca de 1000 UI de heparina sódica. As amostras não conservadas foram mantidas a temperatura ambiente, entre 23 e 25 ºC e aquelas destinadas à refrigeração foram acondicionadas em isopor contendo três litros de água gelada e três quilos de gelo, mantendo-se assim uma temperatura entre zero e 4 ºC. As análises hemogasométricas foram determinadas imediatamente após coleta e 1, 2, 3, 4, 5, 6, 8, 10, 12 e 24 horas após. Verificou-se efeito significativo da temperatura ambiente sobre o conjunto de variáveis, com exceção da concentração sanguínea de HCO3. Quanto às amostras mantidas sob refrigeração, verificou-se efeito significativo sobre a tensão parcial de O2 e CO2 e SO2; sendo que a variação significativa dos valores médios destas variáveis com os valores médios iniciais foram mais tardias em relação às amostras mantidas a temperatura ambiente. Os valores de pH, HCO3 e ABE das amostras refrigeradas mantiveram-se estáveis até 24 horas após a colheita de sangue. Conclui-se, portanto, o exame hemogasométrico de sangue venoso de caprinos pode ser efetivado com segurança até seis horas após sua colheita, desde que mantidos sob refrigeração adequada. | Blood samples collected from 14 healthy, four month-old, male goats of mixed breed, weighting from 30 to 45 kg, were analyzed in order to evaluate the effect of refrigeration on blood gas analysis. Blood samples to be used in the blood gas analysis were collected in duplicates, using disposable needles and plastic syringes containing around 1,000 IU of sodium heparin. Unpreserved samples were kept at room temperature, between 23 and 25 ºC, and those to be kept in refrigeration temperatures were placed in styrofoam coolers containing three liters of cold water and three kilograms of ice, in order to keep temperatures between zero and 4 ºC. Blood gas analyses were carried out immediately after collection and after 1, 2, 3, 4, 5, 6, 8, 10, 12 and 24 hours. Storage at room temperature affected significantly the group of variables studied, except blood concentration of HCO3. When samples were kept under refrigeration, partial pressure of O2, CO2 and SO2 were significantly affected. The significant variation in mean values of these variables when compared with initial mean values was greater in samples kept at room temperature. Results for pH, HCO3- and ABE in the refrigerated samples were stable for up to 24 hours of blood collection. It was concluded, therefore, that blood gas analysis of caprine venous blood may be safely carried out in up to six hours of blood collection, provided that samples are kept under adequate refrigeration.