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Reactivity of polyclonal human CD3 antiserum in lymphoid tissues of cattle, sheep, goats, rats and mice.
1994
Ramos Vara J.A. | Miller M.A. | Lopez E. | Prats N. | Brevik L.
Polyclonal rabbit antiserum to human T-cell CD3 was used to study its reactivity in lymphoid tissues (lymph nodes, spleen, aggregated lymphoid follicles [Peyer's patches], thymus) of several animal species (cattle, sheep, goats, rats, and mice). Using a peroxidase-antiperoxidase technique on formalin-fixed and paraffin-embedded tissues, immunoreactive cells were detected in T cell-dependent areas of the lymphoid tissues. Reactivity was high in all species tested, but mouse tissues had reduced reactivity, compared with the other species. To obtain a reaction, it was necessary to digest tissues with pronase before application of the immunocytochemical technique. Our results indicate that CD3 antiserum may specifically recognize T-lymphoid cells as it does in human lymphoid tissues and can be used as a marker to study physiologic and pathologic conditions of the lymphoid system of these species.
Показать больше [+] Меньше [-]Evaluation of an enzyme-linked immunosorbent assay for diagnosis of paratuberculosis in goats.
1994
Burnside D.M. | Rowley B.O.
A commercial rapid-absorbed ELISA developed to detect antibodies to Mycobacterium paratuberculosis in bovine serum was modified for use with goat serum. Diagnostic sensitivity was evaluated, using a group of 163 goats from a herd with endemic paratuberculosis. Blood and fecal samples were obtained simultaneously, and prevalence of shedding of M paratuberculosis in the feces was estimated by detection of DNA of the mycobacterial insertion sequence, IS900, using a commercial test kit. Diagnostic specificity was evaluated, using blood samples from a total of 123 goats in 10 herds that were considered clinically free of paratuberculosis. The IS900 DNA was detected in 35 of the 163 goats (21%) from the infected herd. Serum antibody to M paratuberculosis was detected in 19 of the 35 IS900 DNA-positive goats, for apparent sensitivity of 54%. Serum antibody was detected in 18 of the 128 IS900 DNA-negative goats from the infected herd. Negative results for serum antibody to M paratuberculosis were obtained for all 123 goats from the herds that were considered clinically free of paratuberculosis.
Показать больше [+] Меньше [-]Development on the esophagus of fetuses and neonates in Korean native goats
1994
Jung, S.H. | Kim, C.S. | Huh, C.K. (Gyeongsang National University, Chinju (Korea Republic). College of Veterinary Medicine)
Ultrastructural studies on the parotid gland of Korean native goat
1994
Lee, H.S. | Lee, I.S. | Kang, T.C. (Seoul National University, Suwon (Korea Republic). College of Veterinary Medicine) | Kim, J.S. (Taegu University, Kyungsan (Korea Republic). College of Rehabilitation)
Development on the rumen of fetuses and neonates in Korean native goats
1994
Huh, C.K. | Kim, C.S. | Kwak, S.D. (Gyeongsang National University, Chinju (Korea Republic). College of Veterinary Medicine)
A study on the epidemiology of caprine anaplasmosis in Korea-(1)-Electron microscopic characterization of the etiologic agent
1994
Baek, B.K. | Jin, C.M. (Chonbuk National University, Chonju (Korea Republic). College of Veterinary Medicine) | Seo, S.Y. | Seo, Y.W. | Kim, D.S. (Livestock Health Research of Chonbuk, Changsu (Korea Republic). Changsu Branch) | Kakoma, I. (Illinois University, Illinois (USA). College of Veterinary Medicine)
Development on the reticulum of fetuses and neonates in Korean native goats
1994
Huh, C.K. | Kim, C.S. | Jung, S.H. (Gyeongsang National University, Chinju (Korea Republic). College of Veterinary Medicine)
Craniometric studies on the skull of Korean native goat
1994
Yi, S.J. (Kyungpook National University, Taegu (Korea Republic). College of Veterinary Medicine) | Lee, H.S. (Seoul National University, Suwon (Korea Republic). College of Veterinary Medicine)
Isolation and preliminary characterization of a caprine rotavirus
1994
Da cost Mendes, V.M. | De Beer, M.C. | Goosen, G.H. (Southern African Medical Univ., Medunsa (South Africa). Dept. of Infectious Diseases and Public Health) | Steele, A.D.
Helminth and arthropod parasites of indigenous goats in the northern Transvaal [South Africa]
1994
Boomker, J. (Southern Africa Medical Univ., Medunsa (South Africa). Dept. of Veterinary Pathology) | Horak, I.G. (Pretoria Univ., Onderstepoort (South Africa). Dept. of Veterinary Diseases) | Ramsay, K.A.