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Studies on effects of lactose on experimental Trypanosoma vivax infection in Zebu cattle. 2. Packed cell volume
2008
Fatihu, M.Y.(Ahmadu Bello University Department of Veterinary Pathology and Microbiology) | Adamu, S.(Ahmadu Bello University Department of Biochemistry) | Umar, I.A.(Ahmadu Bello University Department of Biochemistry) | Ibrahim, N.D.G.(Ahmadu Bello University Department of Veterinary Pathology and Microbiology) | Eduvie, L.O.(Ahmadu Bello University National Animal Production Research Institute) | Esievo, K.A.N.(Ahmadu Bello University Department of Veterinary Pathology and Microbiology)
The ability of intravenously administered lactose in normal saline to prevent a decline in packed cell volume (PCV) during experimental trypanosomosis was studied in Zebu cattle. During the lactose infusion period, the PCV was stable up to Day 5 post-infection (p.i.) in a lactose-infused group, compared to that in an uninfused group in which the PCV dropped significantly (P < 0.05) as shown by the values of cumulative percentage change. Furthermore the mean rate of change in PCV was significantly (P < 0.05) higher in the uninfused group relative to the lactose-infused group during the same period. While the PCV fell markedly in the lactose-infused group a day after lactose infusion was stopped (Day 13 p.i.), subsequent PCV values were significantly (P < 0.05) higher compared to those in the uninfused group, up to the end of experiment on Day 17 p.i. However the mean rates of change in PCV did not vary significantly (P > 0.05) between the groups during the period in which lactose infusion was stopped. The mean levels of parasitaemic waves and parasitaemia were higher, more prolonged and more frequent in the lactose-infused group. It was inferred that the lactose was able to prevent an early onset of anaemia in the Trypanosoma vivax-infected Zebu cattle.
Показать больше [+] Меньше [-]Characterization of Akabane virus (KV0505) from cattle in Korea
2008
Yang, D.K. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea), E-mail: yangdk@nvrqs.go.kr | Kim, Y.H. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea) | Kim, B.H. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea) | Kweon, C.H. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea) | Yoon, S.S. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea) | Song, J.Y. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea) | Lee, S.H. (Jeju Veterinary Research Institute, Jeju, Republic of Korea)
Akabane disease is caused by an arthropod-borne viral pathogen and leads congenital abnormalities of the central nervous system in infected ruminants. One isolate, KV0505, showed cytopathic effect in Vero cells. The KV0505 isolate was obtained from plasma, which was collected from a cattle raised on Jeju Island in May 2005. Jeju Island is located near the southern part of the Korean peninsula. The isolate was confirmed as Akabane virus (AKAV) by immunofluorescence assay using AKAV specific monoclonal antibodies and reverse transcription polymerase chain reaction (RT-PCR). Suckling mice inoculated with the isolate showed signs of paralysis and died within 10 days post-inoculation. Comparisons of the KV0505 N gene sequence with 39 other known AKAV strains revealed nucleotide homologies ranging from 83.6% (MP496 strain) to 99.7% (M171 strain). When compared with the K-9 strain, which was isolated from a cow in Korea in 1994, the nucleotide sequence homology with the N gene was 99.7%. Thus, genes of the KV0505 isolate were closely related to those of the M171 strain, which were clustered into the Ic group of AKAV.
Показать больше [+] Меньше [-]Evaluation of different adjuvants for foot-and-mouth disease vaccine containing all the SAT serotypes
2008
Cloete, M.(Onderstepoort Veterinary Institute) | Dungu, B.(Onderstepoort Veterinary Institute ,Onderstepoort Biological Products) | Van Staden, L.I.(Onderstepoort Veterinary Institute) | Ismail-Cassim, N.(Onderstepoort Veterinary Institute) | Vosloo, W.(Onderstepoort Veterinary Institute)
Foot-and-mouth disease (FMD) is an economically important disease of cloven-hoofed animals that is primarily controlled by vaccination of susceptible animals and movement restrictions for animals and animal-derived products in South Africa. Vaccination using aluminium hydroxide gel-saponin (AS) adjuvanted vaccines containing the South African Territories (SAT) serotypes has been shown to be effective both in ensuring that disease does not spread from the endemic to the free zone and in controlling outbreaks in the free zone. Various vaccine formulations containing antigens derived from the SAT serotypes were tested in cattle that were challenged 1 year later. Both the AS and ISA 206B vaccines adjuvanted with saponin protected cattle against virulent virus challenge. The oil-based ISA 206B-adjuvanted vaccine with and without stimulators was evaluated in a field trial and both elicited antibody responses that lasted for 1 year. Furthermore, the ISA 206 adjuvanted FMD vaccine protected groups of cattle against homologous virus challenge at very low payloads, while pigs vaccinated with an emergency ISA 206B-based FMD vaccine containing the SAT 1 vaccine strains were protected against the heterologous SAT 1 outbreak strain.
Показать больше [+] Меньше [-]Preliminary survey of ticks (Acari: Ixodidae) on cattle in Central Equatoria State, Southern Sudan
2008
Salih, D.A.(Central Veterinary Research Laboratories) | Julla, I.I.(University of Bahr El Ghazal College of Veterinary Science) | Hassan, S.M.(University of Khartoum Faculty of Veterinary Medicine Department of Parasitology) | El Hussein, A.M.(Central Veterinary Research Laboratories) | Jongejan, F.(Utrecht University Faculty of Veterinary Medicine Department of Infectious Diseases and Immunology,University of Pretoria Faculty of Veterinary Science Department of Veterinary Tropical Diseases,)
In a preliminary survey conducted in 2005, the species composition and seasonality of ticks infesting cattle in Central Equatoria State, Southern Sudan was determined. Three locations were selected (Gumbo, Khor Rumla and Nyaing) and surveyed every 3 months. Two cattle herds in each of the three locations were visited four times during the study period. Total body collections of ticks were made from each of five cattle (Nilotic Zebu breed) kept in six different herds. Four tick genera and ten species were identified. The tick species identified were Amblyomma lepidum, Amblyomma variegatum, Boophilus annulatus, Boophilus decoloratus, Hyalomma marginatum rufipes, Hyalomma truncatum, Rhipicephalus appendiculatus, Rhipicephalus evertsi evertsi, Rhipicephalus praetextatus and Rhipicephalus sanguineus group. The highest number of ticks was collected in October during the rainy season. A finding of great significance was that R. appendiculatus, vector of East Coast fever, has now firmly established itself throughout the year with possible implications for cattle production in Central Equatoria State.
Показать больше [+] Меньше [-]Evaluation of biochemical and ultrasonographic measurements as indicators of undernutrition in cattle
2008
Strydom, S.(University of Pretoria Faculty of Veterinary Science Department of Production Animal Studies) | Agenäs, S.(Swedish University of Agricultural Sciences Department of Animal Nutrition and Management Kungsangen Research Centre) | Heath, M.F.(University of Cambridge Department of Veterinary Medicine) | Phillips, C.J.C.(University of Queensland School of Veterinary Science Centre for Animal Welfare and Ethics) | Rautenbach, G.H.(University of Pretoria Faculty of Veterinary Science Department of Production Animal Studies) | Thompson, P.N.(University of Pretoria Faculty of Veterinary Science Department of Production Animal Studies)
Body condition scoring (BCS) gives an indication of the nutritional status of an animal and it is thus an invaluable management tool in domestic livestock systems. It is, however, subjective. This study aimed at identifying biochemical indicators which could be objectively used as an indicator of undernutrition in ruminants. Blood samples were collected from 50 cattle with BCS < 1 and from 50 cattle with BCS > 2.5, using a 0-5 scale, and analysed for albumin, urea, creatinine, fructosamine, beta-hydroxybutyrate, non-esterified fatty acids, total serum protein and haematocrit. Rumpfat and ribfat thickness and marbling relative index were determined ultrasonographically in 15 of the low BCS group and 13 of the high BCS group. The laboratory measure with the best predictive ability for severe undernutrition was albumin, which correctly classified 94 % of cattle, using a cut-off of 31.5 g/l. In contrast to a previous study, our study did not find the fructosamine:albumin ratio to be an accurate test to indicate undernutrition in cattle. Ultrasonic measurement of subcutaneous rumpfat and ribfat proved to reliably predict undernutrition, but may, however be impractical for routine use under most field conditions.
Показать больше [+] Меньше [-]Electrocution caused by a fallen electric wire in Korean native cattles
2008
Bae, Y.C. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea), E-mail: baeyc@nvrqs.go.kr | Lee, K.H. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea) | Yoon, S.S. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea) | Heo, J.H. (Gyeongnam Livestock Promotion Institute South-branch, Tongyoung, Republic of Korea) | Lee, O.S. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea)
We report the electrocution of Korean native cattle by a fallen electric wire, which caused the death of thirteen animals. The owner of the cattle felt an electric shock on touching the steel pen and found a fallen 220-V wire on the roof of the barn; additionally, the roof was singed. Clinically, the animals developed spasm, difficulty breathing, and excessive salivation. Histopathologically, many visceral organs revealed severe congestion or hemorrhage, which is consistent with previous reports. This study revealed that the proper installation of electric wires on farms is essential to prevent economic loss by electrocution.
Показать больше [+] Меньше [-]Use of epidemiologic risk modeling to evaluate control of foot-and-mouth disease in southern Thailand
2008
Wongsathapornchai, K. | Salmān, Muḥammad | Edwards, J.R. | Morley, P.S. | Keefe, T.J. | Van Campen, H. | Weber, S. | Premashthira, S.
Objective--To assess the impacts of the introduction of foot-and-mouth disease (FMD) and various FMD control programs in southern Thailand. Animals--A native population of 562,910 cattle and 33,088 buffalo as well as 89,294 animals legally transported into southern Thailand. Procedures--A quantitative risk assessment was used to ascertain the probability of FMD introduction, and an intrinsic dynamic model was used to assess impacts. Value for the transmission rate (β) was estimated. Five scenarios created to assess the impacts of nonstructural protein (NSP) testing, mass vaccination, and culling were examined. Impacts were assessed through an examination of the estimated annual cumulative incidence (ACI) of FMD. The ACIs of various scenarios were compared by use of the Tukey Studentized range technique. Results--β was estimated at 0.115. Approximately 35,000 cases of FMD would be expected from the baseline situation. A 30% reduction of ACI was detected with the introduction of NSP antibody testing. Prophylactic vaccination resulted in an 85% reduction of ACI. Concurrent use of NSP antibody testing and vaccination reduced the ACI by 96%, and the addition of an eradication policy resulted in a slightly greater decrease in the ACI (98%). Conclusions and Clinical Relevance--The study used epidemiologic models to investigate FMD control interventions. Results suggested that vaccination has more impact than the use of NSP testing. Use of the NSP test reduced ACI during peak seasons, whereas vaccination diminished the underlying incidence. The best mitigation plan was an integrated and strategic use of multiple control techniques.
Показать больше [+] Меньше [-]Comparative studies of histopathologic pulmonary Lesion of cattle and deer tuberculosis by Mycobacterium bovis
2008
Jean, Y.H. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea), E-mail: jean@nvrqs.go.kr | Roh, I.S. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea) | Lee, K.H. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea) | Lee, K.W. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea) | Cho, Y.S. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea) | Joo, Y.S. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea)
Comparative studies of histopathologic lesions from 23 purified protein derivative (PPD) positive cattle, 21 slaughter cattle found with tuberculosis, and 11 tuberculosis-positive elk (Cervus elaphus) were performed. PPD positive cattle did not show specific histopathologic lesions in all 23 heads that were no visible lesion reactor. Slaughter cattle found with tuberculosis revealed microscopically classical granulomatous lesion (tubercle) with central caseous necrosis surrounded by mantle of epithelioid cells and Langhan's giant cells capsuled by connective tissue in lung. Elk was noted with some different lesion patterns with classical granulomatous lesion and suppurative abscesses that was composed of fibrin, degenerated cells without having connective tissue. In addition, many Langhan's giant cells infiltration in alveoli at peripheral lesion were observed in some cases of classical granulomatous lesion and suppurative abscesses.
Показать больше [+] Меньше [-]Idiopathic eosinophilic myositis in Korean native cattle (Bos taurus coreanae)
2008
Rhee, S.H. (Chonbuk National University, Jeonju, Republic of Korea) | Yu, I.J. (Chonbuk National University, Jeonju, Republic of Korea) | Kim, J.H. (Chonbuk National University, Jeonju, Republic of Korea) | Kwon, J.K. (Chonbuk National University, Jeonju, Republic of Korea) | Park, J.H. (Chonbuk National University, Jeonju, Republic of Korea) | You, M.J. (Chonbuk National University, Jeonju, Republic of Korea) | Lee, J.W. (Jeongeup Branch, Institute of Livestock and Veterinary Research, Jeongeup, Republic of Korea) | Park, H.J. (Chonbuk National University, Jeonju, Republic of Korea) | Chekarova, Irina (Chonbuk National University, Jeonju, Republic of Korea) | Camer, Gerry Amor (University of Eastern Philippines, Catarman, Northern Samar, Philippines) | Lim, C.W. (Chonbuk National University, Jeonju, Republic of Korea) | Kim, B.S. (Chonbuk National University, Jeonju, Republic of Korea), E-mail: bskims@chonbuk.ac.kr
Eosinophilic myositis lesions are characterized by severe eosinophil infiltration along muscles of affected animals. The exact cause of the lesion remains controversial and the carcass is condemned once this lesion is seen during meat inspection. A cow slaughtered in Chonbuk province, Korea was observed to have disseminated pale foci throughout the musculature; meat samples were obtained and macroscopically investigated. Cut ends of neck and thigh muscle tissues showed variably sized, multifocal pale white-grayish nodular lesions. Histopathological examination consistently revealed inflammatory lesions with adjacent infiltration of eosinophilic granulocytes and focal necrotic calcification. However, no parasites, including Sarcocystis sp., could be discerned in the affected carcass. This case was diagnosed as idiopathic eosinophilic myositis in cattle.
Показать больше [+] Меньше [-]Molecular characterization of SAT-2 foot-and-mouth disease virus isolates obtained from cattle during a four-month period in 2001 in Limpopo Province, South Africa
2008
Phologane, B.S.(Agricultural Research Council Onderstepoort Veterinary Institute Exotic Diseases Division,Tshwane University of Technology Department of Biomedical Science) | Dwarka, R.M.(Agricultural Research Council Onderstepoort Veterinary Institute Exotic Diseases Division) | Haydon, D.T.(University of Glasgow Division of Environmental and Evolutionary Biology) | Gerber, L.J.(Tshwane University of Technology Department of Biomedical Science) | Vosloo, W.(Agricultural Research Council Onderstepoort Veterinary Institute Exotic Diseases Division,University of Pretoria Faculty of Veterinary Science Department of Veterinary Tropical Diseases)
Foot-and-mouth disease (FMD) is an acute, highly contagious viral infection of domestic and wild cloven-hoofed animals. The virus is a single-stranded RNA virus that has a high rate of nucleotide mutation and amino acid substitution. In southern Africa the South African Territories (SAT) 1-3 serotypes of FMD virus are maintained by large numbers of African buffaloes (Syncerus caffer), which provide a potential source of infection for domestic livestock and wild animals. During February 2001, an outbreak of SAT-2 was recorded in cattle in the FMD control zone of South Africa, adjacent to the Kruger National Park (KNP). They had not been vaccinated against the disease since they form the buffer between the vaccination and free zones but in the face of the outbreak, they were vaccinated as part of the control measures to contain the disease. The virus was, however, isolated from some of them on several occasions up to May 2001. These isolates were characterized to determine the rate of genetic change in the main antigenic determinant, the 1D/2A gene. Nucleotide substitutions at 12 different sites were identified of which five led to amino acid changes. Three of these occurred in known antigenic sites, viz. the GH-loop and C-terminal part of the protein, and two of these have previously been shown to be subject to positive selection. Likelihood models indicated that the ratio of non-synonymous to synonymous changes among the outbreak sequences recovered from cattle was four times higher than among comparable sequences isolated from wildlife, suggesting that the virus may be under greater selective pressure during rapid transmission events.
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