A penta-dog inactivated cell culture vaccine was prepared to protect dogs againstcanine distemper virus, canine parvovirus, canine adenovirus1, 2 and rabies virus.The potency of this vaccine was compared with that of single inactivated vaccinesprepared against each disease, in different groups of susceptible dogs. It was foundthat the protective dose of penta-dog vaccine (2ml) including the protective amounts of the five viral proteins resulted in full protection of vaccinated dogs against the challenge with virulent strain of the used viruses showing no antagonizing effect between each other with and no adverse postvaccinal reaction. So, the prepared inactivated cell culture penta-dog vaccine is a safe and potent vaccine for dogs which resulted in saving time, cost, and effort stress factors on animals and providing good immune statues.

The present study was designed in a trial to use serum replacement instead of thenewborn calf serum in propagation of BHK-21 cell cultures with subsequent reducingthe cost of foot and mouth disease (FMD) vaccine production. Two batches of BHK-21 cell culture were prepared where the medium of the first batch was supplemented with newborn calf serum while the medium of the second batch was supplemented with serum replacement. FMD virus was propagated 7 passages using BHK-21 cell culture. Both virus titration and complement fixation titer (CF) revealed that propagation of FMD virus in cell cultures supplemented with newborn calf serum yields a titre higher than that in case of cells supplemented with serum replacement. Also two batches of FMD inactivated vaccine were prepared from the virus propagated in the two-mentioned cell culture batches. Two groups of susceptible calves were vaccinated with these vaccines. Both of virus neutralization test (VNT) and enzyme linked immunosorbent assay (ELISA) revealed that higher antibody levels were induced in calves vaccinated with the vaccine prepared from cells supplemented with calf serum than those vaccinated with vaccine prepared from cells grown with serum replacement. BHK-21 cell culture supplement with newborn calf serum is most susceptible for FMD virus propagation yielding higher titer of the virus. Moreover, the growth pattern of the used cell culture was much better when the newborn calf serum was used.