In the conditions of the Republic of Belarus there were studied circadian rhythms of some haemotological and biochemical blood indexes of clinically healthy calves and calves with a respiratory syndrome. There was analyzed the efficiency of administration a new complex mineral preparation CMP for correction of internal desynchronoses in complex therapy of calves with respiratory pathologies. Diseases of calves with a respiratory syndrome were accompanied by infringements of circadian fluctuations rhythm (internal desynchronosis) of haemotological and biochemical blood indexes. Application of an antimicrobial preparation Ampicillin for treatment of respiratory diseases of calves promoted the recovery of calves. Introduction of CМP preparation into therapeutic regimen of calves with respiratory syndrome proved to be rather effective; it promoted the correction of metabolic desynchronoses which accompanied the basic disease. Combined application of CМP and Ampicillin made it possible to speed up the clinical recovery of calves and on 30% to prevent animal mortality.

In the conditions of the Republic of Belarus there was studied the formation of nonspecific protection factors of the first year foals. By a principle of conditional analogues there was generated a group of clinically healthy foals at which monthly from bulbar veins there were taken tests of blood for hematological and biochemical analysis. From the obtained data it was visible, that within the first year of foal life there were taking place the processes of formation of nonspecific protection factors of an organism which were accompanied by a gradual increasing of quantity of erythrocytes, haemoglobin, leukocytes, certain change in the diagramme, and also increasing of bactericidal and lysozyme activity of blood serum. However, at seven-month age there was marked a considerable decrease in number of erythrocytes, leukocytes, concentration of haemoglobin, bactericidal and lysozyme activity of blood serum is marked, in leukogram share reduction of segmented neutrophils, and at the same time, the content of eosinocytes and lymphocytes increased. It testified the decreasing of protective forces of an organism during this period. Consequently, the seventh month in the postnatal period of foal life proved to be the most critical.

In the conditions of the Republic of Belarus the seasonal derangements of metabolic processes at high-producing cows usually take place in the period from March till May. Analysis of efficiency of application of national preparation CMP (complex-mineral preparation -iron (15 mg/ml); iodine (6,8 mg/ml); magnesium (6,0 mg/ml); selenium (0,32 mg/ml) and vitamin preparation Trivitim (vitamin A – 30000 IU; , vitamin D – 40000 IU; vitamin E – 20 mg) for the preserving of high milking yielding capacity and prevention of metabolic processes disorders was realized. Four groups of high productive cows were formed. First group was entered 10 ml of CMP intramuscular; cows of the second group were entered 10 ml of Trivitim intramuscular; cows of the third group were entered CMP and Trivitim in the same doses; and the fourth group was a controlled one. On the 1-st, 7-th and 15-th days there was realized evaluation animal clinical state, hematologic and biochemical blood indexes (leukocytes; erythrocytes; hemoglobin; thrombocytes; ESR; total protein; albumins; urea; creatinine; glucose; calcium; phosphorous; magnesium; iron), and average daily milk yield. There was also analyzed the phagocytic activity of neutrophilic leukocytes. Research results showed that content of leukocytes and ESR in blood in all experimental groups was within the standard limits. In the first experimental group there was state the increasing of hemoglobin (from 91,4 +/- 4,38 up to 111,83 +/- 4,3 g/l (P less than0,05) ) and erythrocytes (from 4,48 +/- 0,21 up to 5,97 +/- 0,45 10E12/l) content. The same changes were stated in cows of the 2-d and 3-d experimental groups. Content of hemoglobin and erythrocyte in blood of cows which were administrated with CMP and Trivitim increased from 90,6 +/- 0,06 to 99,83 +/- 3,61 g/l and from 4,51 +/- 0,27 to 5,55 +/- 0,28 10E12/l, respectively. Analyze of biochemical indexes of cow blood showed that content of total protein, calcium, phosphorus, magnesium and iron increased. On the 15-th day there was stated the decrease of urea. Research results proved that the combined application of CMP and Trivitim was efficient and prevented subclinical disorders of metabolic processes

Determination of possible relations between some metabolic activity indexes of Large White pregnant sows (with various pregnancy stages) and their offspring was realized in the conditions of the Republic of Belarus. In the experimental animals there was taken blood for hematologic and biochemical tests. In peripheral blood there was analyzed the average content of hemoglobin, total number of erythrocytes, leukocytes, thrombocytes and corpuscular volume. Content of total protein, albuminolesterin, concentration total kalium and nonorganic phosphorus, magnesium, iron, zinc, activity of alanine- and aspartate transaminase were evaluated in blood serum. Blood tests were made on 84th day of pregnancy, in 30 days before farrowing, on the 2-nd lactation day and on the 2-nd and 35-th days of piglet life. Research results showed high degree of persistence of iron and zinc deficiency of pregnant sows in the conditions of modern national swine production. The obtained results proved the presence of close relationship between metabolic processes of sows and piglets that was revealed in low degree of stores supply with elements which had been in deficit for their mothers

Production examination of efficiency of concentrated vaccine against swine erysipelas from matrix Konev of trial series, control of its immunizing power and production experiments of biological preparation were realized in the conditions of the Republic of Belarus. Research results showed that the presented vaccine had more expressed immunogenic properties in comparison with its production analogue and provided piglets protection against infection. There was analyzed the immunological efficiency of the vaccine: determination of quantity of erythrocytes; leukocytes; content of hemoglobin, level of lysozyme activity, bacterial growth-inhibitory activity, blood serum, phagocytic activity, neutrophilic leukocytes, content of total protein and protein fractions, titre of erysipelas antibodies, and preventive properties of blood serum. Efficiency of biological preparation was determined on the basis of analysis of disease incidence rate, loss of immunized swine caused by swine erysipelas

Investigation of influence of water improved by potassium permanganate solution on welfare and productivity of piglets was realized in the conditions of swine breeding complex in the Republic of Belarus. In the experiment there were used 2 groups of 30-day old piglets. In course of 75 days of experiment the running water of pig complex was used for watering of piglets of control group. Piglets from the experimental group were given water with addition of 0,1% solution of potassium permanganate. Analysis of obtained results showed that water with potassium permanganate promoted the increasing of natural resistance of piglets. At the same time there was stated the increasing of lysozyme and bacterial activityin blood serum. Application of improved water promoted the increasing hemoglobin on 4,8-4,5%, erythrocytes - on 2,9-4,2% in comparison with control. Content of total protein increased on 2,2-2,8% in the experimental groups, lisozyme activity - 6,9-7,0% and bacterial activity - on 5-6,9%. Application of improved water did not influence the glucose and potassium concentration in blood. In the experimental group there was stated the increased lipid concentration on 3,1-6%, cholesterol - on 4,4-6,3%, potassium level - on 2,5-4,5%, phosphorus - on 0,7-5,3%, sodium - on 1,4-3,8%. Application of water with КMnО4 increased average daily weight gain on 5,2-4,2%

Influence of monocalcium phosphate on natural resistance and clinical indexes of blood of breeding bull calves of white-and-black breed was realized in the conditions of the Republic of Belarus. Bull calves of the first (control) group in the composition of their main ration were fed with mixed fodder K-66-b, hay, green mass and as a balanced phosphoric-potassium additive they were fed with8 grams of defluorinated phosphate per 1 kg of mixed feed. Bulls of the second, third and fourth experimental groups in combination with the standard ration instead of difluoridated phosphate were fed with 5; 10; and 15 grams monocalcium phosphate per 1 kg of combined feed, respectively. Research results showed that application of fodder monocalcium phosphate in addition to the main ration made it possible to increase calcium and phosphorus content in blood of experimental bull calves. In the control group phosphorous content was 2,17 micromole, in the experimental groups – 2,40-2,78 micromole; calcium content – 2,50 and 2,58-2,88 micromole, respectively. The analyzed additive rendered positive effect on protein exchange, increased the natural resistance, bacterial growth-inhibitory activity in blood serum in the third, fourth and second groups on 50,3%, 42,5%, and 40,3%, respectively; lysozyme activity – on 46,0%, 39,2%, and 32,3%, respectively; phagocytic activity of leukocytes – on 21,9%, 25%, and 19,1%

The purpose of the research was establishment of hepatic diseases spread in cattle fattening. Trials were conducted with total number of hybrids IV-V generation of black-motley heifer with pedigree Hereford bulls. It has been established the wide spread (22,4%) of hepatic pathology. The leading place among all hepatic pathologies belongs to fatty hepatosis (13,4%) with weakly expressed symptoms, decrease of serum protein level, albumin, glucose and increase of bilirubin, globulins, hepatic enzymes and strong pathology-anatomical and histological manifestations. In the tabular form results of histologic research of liver, some biochemical indicators of blood of healthy and sick animals (M +/- m), the clinical symptoms revealed during the various periods are resulted | Целью исследований являлось изучение структуры и установление широты распространения болезней печени у крупного рогатого скота на откорме. Опыты проведены на широком поголовье помесей IV-V поколения тёлок чёрно-пёстрой породы с племенными быками герефордской породы. Установлено широкое (22,4%) распространение гепатопатий. Ведущее место среди различных форм поражения печени принадлежит жировому гепатозу - 13,4%, морфофункцинальный профиль которого определяется слабо выраженными клиническими симптомами, уменьшением уровня общего белка, альбуминов, глюкозы, повышением концентрации билирубина, активности гепатоспецифических ферментов, а также всех фракций глобулинов, яркой и характерной патологоанатомической и гистологической картиной. В табличной форме приведены результаты гистологического исследования печени, некоторые биохимические показатели крови здоровых и больных животных (M +/- m), клинические симптомы, выявленные в различные периоды

As a result of realized in the conditions of the Republic of Belarus research it was revealed that application of immunostimulative drugs of sodium thyosulphate and vitamin С (ascorbic acid) in combination with a vaccine against salmonellosis, pasteurellosis and streptococcosis of pigs promoted the statistically authentic increasing of leukocytes, absolute quantity of T- and B-lymphocytes content in peripheral blood. Also, it promoted the activation of phagocytic activity and digested ability of neutrophils; activization of mieloblastic hemopoiesis and decreasing of erythrocytopoiesis; activization of morphological reactions in a tissue at a place of administration of the vaccine, lymphatic nodules and spleen. | Проведенные исследования показали, что применение иммуностимуляторов натрия тиосульфата и витамина С с вакциной против сальмонеллеза, пастереллеза и стрептококкоза свиней способствует статистически достоверному повышению в периферической крови содержания лейкоцитов, абсолютного количества Т- и В-лимфоцитов, активизации фагоцитарной активности и перевариваемой способности нейтрофилов; активизации миелобластического кроветворения и снижению эритропоэза; активизации морфологических реакций в ткани на месте введения вакцины, лимфоузлах и селезенке.

Research on determination of changes of morphological and biochemical blood indexes of sheep with fasciolosis after application of the drug Klozantsid (menizol 20%, produced in the Republic of Belarus) was carried out. The research realized in the conditions of the Scientific and Research Institute of Applied Veterinary Medicine and Biotechnology of the Vitebsk State Academy of Veterinary Medicine. In course of the experiment there were used sheep of Romanovskaya breed at the age from 1,5 till 5 years which were spontaneously infected with fasciola. The experimental animals were administrated with the examined antihelminthic preparation. It was found out that after administration of the preparation there was stated the restoration of a level of erythrocytes, haemoglobin, leukocytes, enzymes, proteins and other blood indexes. Klozantsid did not render toxic influence on studied animals. | Проведены исследования по выяснению изменений морфологических и биохимических показателей крови у овец с фасциолёзной инвазией при применении препарата Клозанцид. Исследования проводили в условиях клиники и лаборатории кафедры паразитологии и инвазионных болезней, лаборатории кафедры клинической диагностики, научно-исследовательского института прикладной ветеринарной медицины и биотехнологии учреждения образования «Витебская ордена «Знак Почёта» государственная академия ветеринарной медицины». Объектом исследования являлись овцы романовской породы в возрасте от 1,5 до 5 лет, спонтанно зараженные фасциолами, которым применяли испытуемый препарат, обладающий антигельминтными свойствами. При этом выяснено, что после введения препарата происходит восстановление уровня эритроцитов, гемоглобина, лейкоцитов, ферментов, белков и других показателей. Клозанцид не оказывал токсического влияния на организм животного.