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Determination of the effects of Chia (Salvia hispanica L.) oil and Dandelion (Taraxacum Officinale) extract on Tumor Necrosis Factor-α (TNF-α) and Interleukin 6 (IL-6) release in liver tissue of diabetic rats
2022
Aras, Sükran | Aksu Kilicle, Pinar | Eli̇s Yildiz, Sevda | Uluman, Erhan | Bakir, Buket | Karadag Sari, Ebru | Dag, Serpil
This study aimed to investigate the effects of Chia (Salvia hispanica L.) oil and Dandelion (Taraxacum Officinale) extract on Tumor Necrosis Factor-α (TNF-α) and Interleukin 6 (IL-6) release in liver tissue of diabetic rats. Experimental groups were created as control, sham, chia, dandelion, diabetes (DM), diabetes+chia (DC), and diabetes+dandelion (DD). Body weight and blood glucose measurements were taken on the 1st, 3rd, and 17th days of the study and evaluated statistically. A one-way ANOVA test was performed to determine the differences between the groups. The Duncan test was used to compare significant differences between groups. At the end of the study, Masson's trichrome staining and Hematoxylin-Eosin staining were employed for histological examinations of liver tissues, and the distribution of TNF-α and IL-6 was examined by applying the Streptavidin-biotin peroxidase method.It was determined that body weight and blood glucose measurements were significantly decrease for the DC group compared to other groups. Immunoreactivity of TNF-α and IL-6 was found to decrease in DC and DD groups at close to the control levels.Based on our results, it was thought that the use of chia and dandelion in diabetes may contribute to the alleviation of disease-related complications by having a positive effect on proinflammatory cytokine levels.
Показать больше [+] Меньше [-]The effect Chia seeds (Salvia hispanica l.) on ultrasonographic echogenicity of gastrointestinal tract in cats
2021
Gokdemi̇rel, Burcu | Bozkan, Zeynep
It was aimed to investigate whether chia seeds provide an advantage during ultrasonographic imaging in cats when added to food by means of its high water-holding capacity. Ten healthy cats were ultrasonographically examined following 12- hour fasting period and after feeding with 3 different food types at 1-week intervals. Thus, 4 study group were formed as follows; FST; fasting 12- hour period, WF; feeding with wet cat food following 12- hour fasting period DWF; feeding with ½ of same wet food diluted with the same volume drinking water, CWF; feeding with ½ of same wet food mixed with 36 ml swollen chia seeds obtained by holding 2 hours 3ml chia seeds in 33 ml drinking water. Cats were evaluated ultrasonographically immediately after feeding, and at the 30th minutes and 60th minutes.Especially in terms of thickness, gastric wall evaluation was easier after food intake. In the CWF, the stomach was fully imaged on the frame at all times. The gastrointestinal tract content moved faster when cats were fed DWF or CWF; In the examination immediately after feeding the contents were in duodenum and seen in the jejunum and ileum in the 30- and 60-minutes’ examinations. CWF and DWF revealed more heterogeneous images then WF and also CWF provided better visualization for the intestinal canal wall layer.Ultrasonographic examination after feeding with chia seed added formula can be used as a complementary method following the fasting examination.
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