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A new enzyme-linked immunosorbent assay for serological diagnosis of seal parapoxvirus infection in marine mammals
2022
Badr Yassien | Rahman Md. Matiur | Ohno Yoshito | Ishijima Keita | Maeda Ken | Kohyama Kaoru | Kamatari Yuji O. | Shimizu Kaori | Okada Ayaka | Inoshima Yasuo
Seal parapoxvirus (SPPV) infection has been reported among pinnipeds in aquaria in Japan; however, its seroprevalence is unknown. Therefore, an enzyme-linked immunosorbent assay (ELISA) was developed for serological diagnosis of SPPV infection.
Показать больше [+] Меньше [-]Determination of anti-phage antibodies in calf sera following application of Escherichia coli and Mannheimia haemolytica-specific bacteriophages
2022
Urban-Chmiel Renata | Nowaczek Anna | Chmiel Paulina | Wawrzykowski Jacek | Pyzik Ewelina | Marek Agnieszka | Stępien-Pyśniak Dagmara | Alomari Mohammed M.M. | Dec Marta
The widespread occurrence of drug-resistant bacteria has increased interest in alternatives to antibiotics for combatting bacterial infections, among which bacteriophages play an important role. The ability of phage proteins to induce an anti-phage immune response can significantly limit the effectiveness of treatment, which was the basis for the study described in this article. The aim of the study was to assess the effects of bacteriophages on the induction of an anti-phage humoral response in calves.
Показать больше [+] Меньше [-]Is serological monitoring a fit-for-purpose tool to assess the epidemiological situation of tuberculosis in the sylvatic species of European bison (Bison bonasus) in Poland?
2022
Krzysiak Michał K. | Larska Magdalena | Zabost Anna | Didkowska Anna | Krajewska-Wędzina Monika | Anusz Krzysztof | Augustynowicz-Kopeć Ewa
Bovine tuberculosis is one of the most dangerous zoonotic diseases. Despite the near-complete elimination of the disease from cattle breeding in Poland achieved in 2009, its re-emergence is now observed. Globally, the number of human cases is underestimated and the importance of free-living animals as reservoirs of tuberculosis is growing. As a species highly susceptible to Mycobacterium tuberculosis complex infection, the European bison (Bison bonasus) has a role in the transmission of the disease in Poland. The purpose of the investigation was to assess the epidemiological situation of tuberculosis in Polish European bison serologically.
Показать больше [+] Меньше [-]A new enzyme-linked immunosorbent assay for serological diagnosis of seal parapoxvirus infection in marine mammals
2022
Badr, Yassien | Rahman, Md Matiur | Ōno, Yoshito | Ishijima, Keita | Maeda, Ken | Kohyama, Kaoru | Kamatari, Yuji O. | Shimizu, Kaori | Okada, Ayaka | Inoshima, Yasuo
Seal parapoxvirus (SPPV) infection has been reported among pinnipeds in aquaria in Japan; however, its seroprevalence is unknown. Therefore, an enzyme-linked immunosorbent assay (ELISA) was developed for serological diagnosis of SPPV infection. The gene encoding the major envelope protein of SPPV was cloned into the eukaryotic expression vector pAcGFP1-N1, which encodes the green fluorescence protein (GFP), thereby producing a fusion protein (Env-GFP). Parental and cloned vector DNA was independently transfected into cultured seal cells for the expression of GFP and Env-GFP. The wells of an ELISA plate were coated with either GFP- or Env-GFP-transfected cell lysates. The light absorbance of each serum sample was adjusted by subtracting the absorbance of GFP-coated wells from that of Env-GFP-coated wells. Sera from two spotted seals (Phoca largha), six beluga whales (Delphinapterus leucas), three Pacific white-sided dolphins (Lagenorhynchus obliquidens), and ten bottlenose dolphins (Tursiops truncatus) from an aquarium in Japan were examined using the ELISA. Positive reactions were not observed, except in one preserved sample collected ten years ago from a naturally SPPV-infected spotted seal. The established ELISA could be useful in screening marine mammal sera for anti-SPPV antibodies.
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