Introduction: Hard antlers of deer are unique bioindicators of environmental metal pollutions, but sampling methods presented in the literature are inconsistent. Due to the specific growth pattern of antlers and their histological structure, sampling methods described in the literature were reviewed, the suitability of using mixed samples of both antler layers as element bioindicators was assessed, and the codified method of antler sampling used for bioindication was described. Material and Methods: Lead, cadmium, mercury, arsenic, copper, zinc, and iron in trabecular and cortical parts of hard antlers of red deer (Cervus elaphus) were determined using different methods of atomic absorption spectrometry (depending on the element). Results: Mean mercury content in trabecular bone (0.010 ±0.018 mg/kg) was 5 times higher than in cortical bone (0.002 ±0.003 mg/kg). Mean iron concentration was approximately 15 times higher in trabecular (239.83 ±130.15 mg/kg) than in cortical bone (16.17 ±16.44 mg/kg). Concentrations of other analysed elements did not differ statistically between antler layers. Conclusion: In mixed antler samples, concentrations of mercury and iron depend on the particular antler layer contents. This therefore warrants caution when comparing results across studies and specification of the sampling methodology of antlers is highly recommended.

Introduction: Hard antlers of deer are unique bioindicators of environmental metal pollutions, but sampling methods presented in the literature are inconsistent. Due to the specific growth pattern of antlers and their histological structure, sampling methods described in the literature were reviewed, the suitability of using mixed samples of both antler layers as element bioindicators was assessed, and the codified method of antler sampling used for bioindication was described. Material and Methods: Lead, cadmium, mercury, arsenic, copper, zinc, and iron in trabecular and cortical parts of hard antlers of red deer (Cervus elaphus) were determined using different methods of atomic absorption spectrometry (depending on the element). Results: Mean mercury content in trabecular bone (0.010 ±0.018 mg/kg) was 5 times higher than in cortical bone (0.002 ±0.003 mg/kg). Mean iron concentration was approximately 15 times higher in trabecular (239.83 ±130.15 mg/kg) than in cortical bone (16.17 ±16.44 mg/kg). Concentrations of other analysed elements did not differ statistically between antler layers. Conclusion: In mixed antler samples, concentrations of mercury and iron depend on the particular antler layer contents. This therefore warrants caution when comparing results across studies and specification of the sampling methodology of antlers is highly recommended.

Poultry layer houses are mostly open house system that generally faced with flies and odour and has become nuisance factors to society and the environment. This study was aimed to determine the effectiveness of applying EM in controlling pollutant gas emission at poultry layer house. A commercial poultry layer farmwas selected. Two treatments were carried out, the control without any application of microorganisms and the treated group with commercial microorganism application. Atmospheric ammonia and hydrogen sulphide were measured using a special instrument called ‘multi-gas detector’. The levels of ammonia and hydrogensulphide were measured twice a week. Odour production cannot be completely prevented on a farm. Therefore, most odour control methods are designed to keep or dissipate odours within the farm boundary, thus minimising odour complaints from surrounding neighbours. This paper reports that applying of effective microorganisms into the manure showed some positive influence in controlling atmospheric ammonia levels.

Biogas plant is an anaerobic digester that produces biogas fromorganic materials. The general purpose of implementing biogas plant in an animal farm is to obtain biogas from animal manure and the effluent of biogas digester after fermentation process can be used as organic fertiliser. There are 15 biogas plants based on livestock waste that have been developed throughout Malaysia until 2018. Two more biogas plants are planned to be built in Segamat and Keningau. All biogas plants built were originally aimed for domestic use which is as source of energyused for cooking and incinerator as well as to generate electricity for farm use. Out of 15 biogas plants, 7 plants were built on cattle farms, 4 plants on pig farms, 3 plantson poultry farms and only 1 plant on buffalo farm. Total construction cost for each biogas plant is between MYR4,500 to MYR30 million. Based on the survey conducted, it revealed that only 40% of the plants are still functional. However, 60% were dormant or nonfunctional due to poor maintenance, design errors, lack of technical knowledge to run the system consistently, improper planning and monitoring or insufficient livestock waste to continuously producing biogas.

A biogas plant is an anaerobic digester that produces biogas from organic materials such as animal waste, sewage slurry, vegetable waste and others. The Department of Veterinary Services had developed a small-scale biogas plant at a commercial dairy farm
in Semenyih, Selangor. The objectives of this project were to promote green technology and zero waste concepts on animal farming as well as to mitigate adverse effects on the environment due to unsystematic management of animal waste disposal. The biogas produced was used as fuel for biogas lamp, biogas stove, biogas water heater, biogas rice cooker and 1 kW biogas generator. The generator was connected to the distribution board in order to supply electricity to a few switch sockets and lighting system for farm use. A biogas plant in an animal farm is one of the green technology applications because it not only produces renewable gas and generates
electricity but also minimises greenhouse gas emissions and environmental pollution. Furthermore, the residual solid waste
produced at the end of the process can be dried to be used as organic fertiliser.